Comparative morphofunctional study of dispersed mature canine cutaneous mast cells and BR cells, a poorly differentiated mast cell line from a dog subcutaneous mastocytoma

García, G.; Brazı́s, Pilar; Majó, Natàlia; Ferrer, L.; Mora, Fernando; Puigdemont, Anna

doi:10.1016/s0165-2427(97)00166-9

Cited by 24 publications

(39 citation statements)

References 20 publications

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“…In this study, CoMS cells showed the formalinsensitivie, diverse cytoplasmic granules in their ultrastructural findings and the lack of response to compound 48/80. These characteristics were similar to those of the most of canine mast cells in the gastric mucosa [14,15] and the airway lumen [16], but not in the skin [4]. This may be related to that the original lesion where isolated CoMS cells is the mucosal site in the lower lip.…”

supporting

confidence: 65%

“…This result suggests that CoMS cells have few functional IgE receptors like the neoplastic mast cell lines such as human HMC-1, mouse P-815 and canine BR [4,10,12]. Concanavalin A elicited cell-to-cell aggregation of CoMS cells and significant histamine release independent on IgE.…”

mentioning

confidence: 95%

“…Study of histamine content and release was examined as described previously [4]. Compound 48/80 (ICN Biomedicals Inc., Ohio, U.S.A.), calcium ionophore A23187 (CAL-BIOCHEM, Darmstadt, Germany), substance P (BACHEM AG, Bubendorf, Switzerland), anti-canine IgE (ViroStat, Portland, ME, U.S.A.) and concanavalin A (Wako Pure Chemical Ind., Osaka, Japan) were used as stimuli.…”

mentioning

confidence: 99%

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Establishment and Characterization of a New Canine Mast Cell Tumor Cell Line.

et al. 2001

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ABSTRACT. A new cell line (CoMS) was established from a 3-year-old male mongrel dog with mast cell tumor of the oral mucosa. CoMS cells grow in suspension with a doubling time of 27.0 ± 0.7 hr. The cytoplasmic granules were formalin-sensitive, showed diverse appearances in their ultrastructural findings and contained heparin proteoglycan and neutral protease chymase. Calcium ionophore A23187, substance P and concanavalin A caused significant histamine release from CoMS cells, while compound 48/80 failed to release histamine. This cell line will make an available source for studies on canine mast cell tumors. KEY WORDS: canine, cell line, mast cell tumor.J. Vet. Med. Sci. 63(9): 1031-1034, 2001 Mast cell tumor (MCT) is one of the common neoplasms in dogs, accounting for 7% to 21% of all skin tumors and 11% to 27% of all malignant skin tumors [19]. There are still some dilemmas caused by its unpredictable biological behaviors with regard to diagnosis and treatment of MCT [9]. Studies on several neoplastic cell lines of human or rodent mast cells such as HMC-1, P-815 and RBL-2H3 have provided many informations about mast cell biology [17,18,20]. There will be anatomical, biochemical, immunological and pharmacological differences between human, rodent and canine mast cells. Available cell lines of canine MCT are therefore required for the research on this disease.In this paper, we describe a successful establishment of a new canine MCT cell line named CoMS in continuous culture and report morphology, histamine release activity and granular components such as proteases and heparin of CoMS cells.CoMS cell line was obtained from a mucosal mass in the lower lip of a 3-year-old male mongrel dog with MCT. The dog was referred to the Veterinary Teaching Hospital of Hokkaido University with massive swelling of a left lower lip and both cervical lymph nodes. The clinical managements were consisted of surgical excision, radiotherapy and chemotherapy. In spite of these treatments, the dog died of deterioration of general conditions 56 days after the first admission. Multiple gastroduodenal ulcers and multiple distant metastases to the internal organs such as the liver, spleen, kidneys and lungs but no metastasis to the skin were observed at necropsy.The specimen of CoMS collected at surgery was initially maintained in vivo passage in 5-week-old female C.B-17 severe combined immunodeficiency (SCID) mice (Clea Lab., Tokyo, Japan) by subcutaneous implantation, then the tumor tissues excised from the mice were used for establishment of the cell line in vitro.These tumor tissues were minced finely in RPMI 1640 medium containing 2 mg/ml sodium bicarbonate, 25 mM Hepes buffer, 100 U/ml penicillin and 100 µg/ml streptomycin, filtrated through a stainless mesh and washed two times in phosphate buffered saline. The isolated cells were then suspended and incubated in the above RPMI 1640 medium with 10% heat inactivated fetal calf serum (FCS) at 37°C in a humidified atmosphere of 5% CO 2 and were passaged every 4 to 6 day. The cell line n...

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confidence: 65%

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confidence: 95%

mentioning

confidence: 99%

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Establishment and Characterization of a New Canine Mast Cell Tumor Cell Line.

et al. 2001

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“…In contrast nonimmunologic stimulation of both cell lines resulted in similar concentration-dependent histamine release (12,33). Giemsa, Wright).…”

Section: Canine Mast Cell Tumours Epidemiology and Aetiologymentioning

confidence: 92%

Mast cells and canine mast cell tumours. A review

Misdorp

2004

Veterinary Quarterly

101

103

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“…Kambe et al [18] reported a successful culture system for proliferation of mature SMCs derived from dispersed human skin samples in a serum-free culture medium supplemented with recombinant human SCF. In dogs, cell lines derived from mast cell tumors have been used as a pure population of mast cells, but malignant transformation substantially alters the structure and function of mast cells [12,24]. A procedure to culture canine bone marrow-derived mast cells (BMMCs) in order to obtain non-neoplastic progenitor cells has recently been reported [23].…”

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confidence: 99%

Cultivation and Characterization of Canine Skin-Derived Mast Cells

Kawarai

Masuda

Ohmori

et al. 2010

The Journal of Veterinary Medical Science

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ABSTRACT. It is essential to develop a technique to culture purified skin-derived mast cells (SMCs) to facilitate immunological research on allergic diseases in dogs. This study was performed to develop an efficient culture system for canine SMCs and to characterize the cells in comparison to canine bone marrow-derived mast cells (BMMCs). Enzymatically digested skin biopsy samples were cultivated in serum-free AIM-V medium supplemented with recombinant canine stem cell factor. Three to five weeks after the initiation of culture, mast cells were collected by a magnetic activated cell separation system using anti-c-Kit antibody. The collected cells were composed of a uniform population showing morphological characteristics of mast cells with a round or oval nucleus and abundant toluidine bluepositive metachromatic granules in the cytoplasm. The results of flow cytometric analysis for the presence of cell membrane c-Kit and Fc epsilon receptor I (FcRI) indicated that approximately 90% of the cells were mast cells. The cytoplasmic granules were positive for both tryptase and chymase. Apparent dose-dependent degranulation was induced by antibody-mediated cross-linking of immunoglobulin E (IgE) bound to the cells. These cytological and immunological characteristics observed in SMCs were mostly similar to those observed in BMMCs; however, IgE-mediated degranulation was significantly lower in SMCs than BMMCs. The culture system for canine SMCs developed in this study would be useful in understanding the pathophysiology and developing anti-allergic therapeutics in canine allergic dermatitis.KEY WORDS: bone marrow, canine, culture, mast cells, skin.J. Vet. Med. Sci. 72(2): 131-140, 2010 Allergic dermatitis in dogs is a complex of inflammatory skin diseases primarily mediated by type I hypersensitivity [8]. Cross-linking of the surface Fc epsilon receptor I (FcRI)-bound immunoglobulin E (IgE) with allergens results in the release of various mediators from mast cell granules, inducing type I hypersensitivity [5,6]. Studies using an experimental allergic model have revealed that cytokines and chemokines released from mast cells induce infiltration of leukocytes, leading to a late-phase reaction [42].In dogs, histopathological examination of the skin lesions of atopic dermatitis (AD) has revealed infiltration of mast cells, Langerhans cells, and lymphocytes in addition to a small number of eosinophils and neutrophils into the lesional skin [14]. The presence of allergen-specific IgEbearing mast cells in the dermis of allergic dogs has been shown [13], and both the number of mast cells and the histamine concentration in the dermis have been shown to correlate with clinical symptoms of AD [28]. As shown in the spontaneously developed AD in dogs, in a canine experimental model of AD, epicutaneous application of an allergen slurry has been shown to generate a penetration of allergen into the dermis, an elevated allergen-specific serum IgE, and localized pruritic dermatitis with typical histological changes by IgE-induced inf...

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Comparative morphofunctional study of dispersed mature canine cutaneous mast cells and BR cells, a poorly differentiated mast cell line from a dog subcutaneous mastocytoma

Cited by 24 publications

References 20 publications

Establishment and Characterization of a New Canine Mast Cell Tumor Cell Line.

Establishment and Characterization of a New Canine Mast Cell Tumor Cell Line.

Mast cells and canine mast cell tumours. A review

Cultivation and Characterization of Canine Skin-Derived Mast Cells

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