Comparative metabolome and transcriptome analyses of the properties of Kluyveromyces marxianus and Saccharomyces yeasts in apple cider fermentation

Zhang, Zhiyong; Lan, Qing; Yu, Yao; Zhou, Jungang; Lü, Hong

doi:10.1016/j.fochms.2022.100095

Cited by 8 publications

(4 citation statements)

References 31 publications

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“…Meanwhile, we found that several DEGs were involved in p53 signaling pathway, FoxO signaling pathway, MAPK signaling pathway, and NF-κB signaling pathway. Our nding is consistent with the previous report [43], indicating that these pathways participate in Cd-induced cell apoptosis. In summary, our transcriptional results suggested that DEGs are involved in several apoptosis-related pathways in Cd exposed cells.…”

Section: Transcriptional Response Of Gc-1 Spg Cells To CD Exposuresupporting

confidence: 94%

Cadmium exposure causes mouse spermatogonia apoptosis via inducing endoplasmic reticulum stress

Wu,

Yin,

Wang

et al. 2024

Preprint

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Cadmium (Cd), which can cause testicular germ cell apoptosis, is a well-recognized male reproductive toxicant, but the underlying mechanism still needs investigation. To detect Cd toxicity on testicular germ cell, we treated mouse spermatogonia with CdCl2 in vitro and investigated the responses from cells at both RNA and protein levels. After treating mouse-derived spermatogonia cell line GC-1 spg cells with 20 µM CdCl2 for 24h, cell apoptosis was measured by TUNEL and flow cytometry assay. After then, the expressions of key genes and protein biomarkers involved in endoplasmic reticulum (ER) stress were detected by qPCR and western blot, respectively. Finally, untargeted metabolomics was performed to compare metabolic differences, and Illumina RNA sequencing was conducted to screen differentially expressed genes (DEGs). Our results indicated that Cd exposure caused cell apoptosis, DEGs were involved in several apoptosis-related pathways. Cd exposure apparently elevated the mRNA and protein expressions levels of both GRP78 and ATF6α, and disrupted the expression of many types of metabolites, especially for amino acids. Taken together, our study uncovers the pathway of Cd toxicity on mouse spermatogonia, provides deep understanding on Cd-induced testicular toxicity.

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Section: Transcriptional Response Of Gc-1 Spg Cells To CD Exposuresupporting

confidence: 94%

Cadmium exposure causes mouse spermatogonia apoptosis via inducing endoplasmic reticulum stress

Wu,

Yin,

Wang

et al. 2024

Preprint

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“…Yeast is applied as a starter culture in bread and cheeses, as well as in beer, wine and other alcoholic fermentation products, but they can also propose spoilage in foods, such as yogurt, salads, fruit juice and mayonnaise [270][271][272][273]. In addition to being extensively applied in the production of beverages, foods and pharmaceuticals, yeasts play significant functions as model eukaryotic cells in improving our knowledge in the biomedical and biological sciences [274][275][276][277].…”

Section: Eukaryotic Microorganism Species and Fermentation Technologymentioning

confidence: 99%

Research Progress of Fermented Functional Foods and Protein Factory-Microbial Fermentation Technology

2022

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Fermentation has been used for ages as a safe technique for food preservation, and it uses minimal resources. Fermentation is related to a wide range of catabolic biochemical procedures in both eukaryotes and prokaryotes. Yeasts are eukaryotes; they can use oxygen while also having the ability to live without oxygen. The lactate fermentation process consists of glycolysis and some alternative steps. A review of the literature was done using keywords in main indexing systems, including PubMed/MEDLINE, Scopus, the search engine of the Institute for Scientific Web of Science and Google Scholar. The keywords reviewed were fermentation technologies, protein mass expression, health benefits of functional foods, microbial fermentation technology, anaerobic respiration, fermentation in eukaryotes, fermentation in prokaryotes, solid state fermentation and submerged fermentation. This research was carried out to highlight the importance of fermentation technology and to introduce and survey the technology and its relationship with functional foods. Research progress in the area of protein factory-microbial fermentation technology was also investigated and inspected.

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“…The mixed fermentation system of LAB and yeast is used to improve the aroma intensity [ 18 ], increase the nutritional value [ 19 ], and produce functional components [ 20 ]. Sensory characteristics deeply depend on the composition and content of aroma compounds as well as nonvolatile secondary metabolites produced in the fermentation process [ 21 ]. The analysis of characteristic flavor compounds in mixed fermentation is helpful to understand the fermentation performance of mixed fermentation and its influence on cheese metabolic profile, which is conducive to the directional control of the production of characteristic cheese.…”

Section: Introductionmentioning

confidence: 99%

Differences between Kazak Cheeses Fermented by Single and Mixed Strains Using Untargeted Metabolomics

Wang

et al. 2022

Foods

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Mixed fermentation improves the flavor quality of food. Untargeted metabolomics were used to evaluate the impact of mixed fermentation and single-strain fermentation on the volatile and non-volatile compound profiles of Kazak cheese. Lacticaseibacillus paracasei SMN-LBK and Kluyveromyces marxianus SMN-S7-LBK were used to make mixed-fermentation cheese (M), while L. paracasei SMN-LBK was applied in single-strain-fermentation cheese (S). A higher abundances of acids, alcohols, and esters were produced via mixed fermentation. Furthermore, 397 differentially expressed non-volatile metabolites were identified between S and M during ripening. The flavor compounds in mixed-fermentation cheese mainly resulted from ester production (ethyl butanoate, ethyl acetate, ethyl octanoate, and ethyl hexanoate) and amino acid biosynthesis (Asp, Glu, Gln, and Phe). The metabolites were differentially expressed in nitrogen metabolism, D-glutamine and D-glutamate metabolism, phenylalanine metabolism, D-alanine metabolism, and other metabolic pathways. The amount of flavor compounds was increased in M, indicating that L. paracasei SMN- LBK and K. marxianus SMN-S7-LBK had synergistic effects in the formation of flavor compounds. This study comprehensively demonstrated the difference in metabolites between mixed-fermentation and single-strain-fermentation cheese and provided a basis for the production of Kazak cheese with diverse flavor characteristics.

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Comparative metabolome and transcriptome analyses of the properties of Kluyveromyces marxianus and Saccharomyces yeasts in apple cider fermentation

Cited by 8 publications

References 31 publications

Cadmium exposure causes mouse spermatogonia apoptosis via inducing endoplasmic reticulum stress

Cadmium exposure causes mouse spermatogonia apoptosis via inducing endoplasmic reticulum stress

Research Progress of Fermented Functional Foods and Protein Factory-Microbial Fermentation Technology

Differences between Kazak Cheeses Fermented by Single and Mixed Strains Using Untargeted Metabolomics

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