Comparative investigation on antibacterial, biological and mechanical behaviour of monticellite and diopside derived from biowaste for bone regeneration

Choudhary, Rajan; Krishnamurithy, G.; Raghavendran, Hanumantha Rao Balaji; Murali, Malliga Raman; Kamarul, Tunku; Suresh, Anushree; Abraham, Jayanthi; Praharaj, Subhashree; Swamiappan, Sasikumar

doi:10.1016/j.matchemphys.2022.126157

Cited by 15 publications

(17 citation statements)

References 61 publications

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“…The observed phase matched the ICDD card № 01-075-1092. The FT-IR spectrum ( Figure 1 B) was found to be in good agreement with recently published diopside characterization results, demonstrating the presence of four functional groups’ peaks in the 650–4000 cm −1 range [ 34 ]. According to SEM micrographs ( Figure 1 C,D) the formed product is composed of aggregated porous microparticles.…”

Section: Resultssupporting

confidence: 88%

Antibacterial and Anti-Biofilm Properties of Diopside Powder Loaded with Lysostaphin

et al. 2023

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Background: Diopside-based ceramic is a perspective biocompatible material with numerous potential applications in the field of bone prosthetics. Implantable devices and materials are often prone to colonization and biofilm formation by pathogens such as Staphylococcus aureus, which in the case of bone grafting leads to osteomyelitis, an infectious bone and bone marrow injury. To lower the risk of bacterial colonization, implanted materials can be impregnated with antimicrobials. In this work, we loaded the antibacterial enzyme lysostaphin on diopside powder and studied the antibacterial and antibiofilm properties of such material to probe the utility of this approach for diopside-based prosthetic materials. Methods: Diopside powder was synthesized by the solid-state method, lysostaphin was loaded on diopside by adsorption, the release of lysostaphin from diopside was monitored by ELISA, and antibacterial and anti-biofilm activity was assessed by standard microbiological procedures. Results and conclusions: Lysostaphin released from diopside powder showed high antibacterial activity against planktonic bacteria and effectively destroyed 24-h staphylococcal biofilms. Diopside-based materials possess a potential for the development of antibacterial bone grafting materials.

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Section: Resultssupporting

confidence: 88%

Antibacterial and Anti-Biofilm Properties of Diopside Powder Loaded with Lysostaphin

et al. 2023

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“…The formation of nanocrystalline HA (16.8 and 79.8 nm) and Mg-WH (62–81.6 nm) were initially assessed based on the calculation of crystallite at size (002) and (0210) plane for HA and Mg-WH, respectively, from the XRD analysis (see Table 4 ). The literature shows that the structural analysis of synthesised HA is usually focused on the (002) reflection at 25.8 (2θ) and the planes (112 and 300) between 30° and 35° (2θ) [ 18 , 19 ]. However, poor resolution of peaks in the later range (30°–35° (2θ)) is usually observed for low-crystalline materials, and hence it can be difficult to distinguish between them [ 18 , 19 ].…”

Section: Discussionmentioning

confidence: 99%

“…The literature shows that the structural analysis of synthesised HA is usually focused on the (002) reflection at 25.8 (2θ) and the planes (112 and 300) between 30° and 35° (2θ) [ 18 , 19 ]. However, poor resolution of peaks in the later range (30°–35° (2θ)) is usually observed for low-crystalline materials, and hence it can be difficult to distinguish between them [ 18 , 19 ]. A similar phenomenon was found for the sample CaP(N-Am)200 in this study, as shown in Figure 1 A, where low resolution of the peaks between 30° and 35° (2θ) (at the (112) and (300) plane) were observed and could hardly be differentiated.…”

Section: Discussionmentioning

confidence: 99%

“…In this context, the valorisation of wastes is of utmost importance because this potential alternative approach will reduce waste disposal and use natural resources for new materials development, which will increase the economic value of products. Some of these wastes could be utilised for the development of biomaterials such as calcium phosphates (CaPs) [ 16 , 17 , 18 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

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Production of Nano Hydroxyapatite and Mg-Whitlockite from Biowaste-Derived products via Continuous Flow Hydrothermal Synthesis: A Step towards Circular Economy

et al. 2023

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Biowastes from agriculture, sewage, household wastes, and industries comprise promising resources to produce biomaterials while reducing adverse environmental effects. This study focused on utilising waste-derived materials (i.e., eggshells as a calcium source, struvite as a phosphate source, and CH3COOH as dissolution media) to produce value-added products (i.e., calcium phosphates (CaPs) derived from biomaterials) using a continuous flow hydrothermal synthesis route. The prepared materials were characterised via XRD, FEG-SEM, EDX, FTIR, and TEM analysis. Magnesium whitlockite (Mg-WH) and hydroxyapatite (HA) were produced by single-phase or biphasic CaPs by reacting struvite with either calcium nitrate tetrahydrate or an eggshell solution at 200 °C and 350 °C. Rhombohedral-shaped Mg-WH (23–720 nm) along with tube (50–290 nm diameter, 20–71 nm thickness) and/or ellipsoidal morphologies of HA (273–522 nm width) were observed at 350 °C using HNO3 or CH3COOH to prepare the eggshell and struvite solutions, and NH4OH was used as the pH buffer. The Ca/P (atomic%) ratios obtained ranged between 1.3 and 1.7, indicating the formation of Mg-WH and HA. This study showed that eggshells and struvite usage, along with CH3COOH, are promising resources as potential sustainable precursors and dissolution media, respectively, to produce CaPs with varying morphologies.

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“…Their multifunctional properties recommend them as candidates for the development of materials suitable for the treatment of bone tissue injuries, as well as its regeneration [ 12 , 13 , 14 , 15 , 16 , 17 ]; this is due to Ca and Mg ions [ 1 , 18 ] that promote the process of mineralization through apatite deposition [ 3 , 19 ] and enhance cell proliferation and differentiation [ 1 , 20 , 21 ]. Some researchers prepared larnite and rankinite through the sol–gel combustion method [ 22 ], but also monticellite and diopside from eggshell waste via the combustion route [ 23 ], with good results in terms of mechanical strength, bioactivity, antibacterial activity, as well as cell adhesion, proliferation and differentiation. Sodium calcium silicate is another bioactive ceramic that was synthesized by the combustion technique and whose bioactivity was found to be rapid when compared with that of calcium silicates and calcium magnesium silicates [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

Processing of Calcium Magnesium Silicates by the Sol–Gel Route

Alecu

Costea

Surdu

et al. 2022

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In this work, calcium magnesium silicate ceramics were processed through the sol–gel method in order to study the crystalline and morphological properties of the resulting materials in correlation with the compositional and thermal parameters. Tetraethyl orthosilicate and calcium/magnesium nitrates were employed as sources of cations, in ratios specific to diopside, akermanite and merwinite; they were further subjected to gelation, calcination (600 °C) and thermal treatments at different temperatures (800, 1000 and 1300 °C). The properties of the intermediate and final materials were investigated by thermal analysis, scanning electron microscopy, energy dispersive X-ray spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction and Rietveld refinement. Such ceramics represent suitable candidates for tissue engineering applications that require porosity and bioactivity.

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Comparative investigation on antibacterial, biological and mechanical behaviour of monticellite and diopside derived from biowaste for bone regeneration

Cited by 15 publications

References 61 publications

Antibacterial and Anti-Biofilm Properties of Diopside Powder Loaded with Lysostaphin

Antibacterial and Anti-Biofilm Properties of Diopside Powder Loaded with Lysostaphin

Production of Nano Hydroxyapatite and Mg-Whitlockite from Biowaste-Derived products via Continuous Flow Hydrothermal Synthesis: A Step towards Circular Economy

Processing of Calcium Magnesium Silicates by the Sol–Gel Route

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