Comparative investigation of vitamins and their analogues on terminal differentiation, from preadipocytes to adipocytes, of 3T3-L1 cells

The Vitamin K Epoxide Reductase Vkorc1l1 Promotes Preadipocyte Differentiation in Mice

“…Consistent with our data, rats fed with either extra vitamin K 1 or K 2 displayed decreased body fat and serum TG . Considering that local tissues are likely capable of converting vitamin K 1 to vitamin K 2 in animals , these results support that vitamin K 2 plays the major role in impeding adipogenesis in mammals . As we have shown, vitamin K 2 could suppress the expression of Pparg2 , which is a key regulator in promoting adipogenesis (Supporting Information Figure S5A).…”

“…A previous report showed that vitamin K 1 could not inhibit the differentiation of preadipocytes in vitro . In our study, we have shown that vitamin K 2 but not vitamin K 1 suppressed adipogenesis of SVF cells.…”

The Vitamin K Epoxide Reductase Vkorc1l1 Promotes Preadipocyte Differentiation in Mice

“…Consistent with our data, rats fed with either extra vitamin K 1 or K 2 displayed decreased body fat and serum TG . Considering that local tissues are likely capable of converting vitamin K 1 to vitamin K 2 in animals , these results support that vitamin K 2 plays the major role in impeding adipogenesis in mammals . As we have shown, vitamin K 2 could suppress the expression of Pparg2 , which is a key regulator in promoting adipogenesis (Supporting Information Figure S5A).…”

“…A previous report showed that vitamin K 1 could not inhibit the differentiation of preadipocytes in vitro . In our study, we have shown that vitamin K 2 but not vitamin K 1 suppressed adipogenesis of SVF cells.…”

Retinoic acid modulates lipid accumulation glucose concentration dependently through inverse regulation of SREBP‐1 expression in 3T3L1 adipocytes

“…In the presence of RA, 3T3-L1 cells cultured in 5.5 mM glucose accumulated lipids less than those cultured in the absence of RA, accompanied with decreases in the mRNA expression of FAS and aP2 ( Fig. 1A-D), indicating that RA suppressed the adipogenic differentiation of mouse 3T3L1 preadipocytes cultured in normal glucose medium as previously reported (Kawada et al 1990;Schwarz et al 1997). Surprisingly, the cells cultured in 25 mM glucose in the presence of RA enhanced lipid accumulation more than those cultured in the absence of RA, accompanied with increases in the mRNA expression of FAS, ACC1a and aP2 ( Fig.…”

Controlled conversion of an immortalized mesodermal progenitor cell towards osteogenic, chondrogenic, or adipogenic pathways.

“…When AA (50 ixg/ml) is added to Dex-treated aggregates, the nodules becomes larger and more adherent but the kinetics of chondrogenic differentiation is not significantly reduced and clusters of adipocytes reproducibly appear within 25 d at the periphery of the spreading aggregates. This effect of AA, as a potent activator of adipose cell differentiation was previously observed (Ono et al, 1990;Kawada et al, 1990).…”

The Vitamin K Epoxide Reductase Vkorc1l1 Promotes Preadipocyte Differentiation in Mice

“…Consistent with our data, rats fed with either extra vitamin K 1 or K 2 displayed decreased body fat and serum TG . Considering that local tissues are likely capable of converting vitamin K 1 to vitamin K 2 in animals , these results support that vitamin K 2 plays the major role in impeding adipogenesis in mammals . As we have shown, vitamin K 2 could suppress the expression of Pparg2 , which is a key regulator in promoting adipogenesis (Supporting Information Figure S5A).…”

“…A previous report showed that vitamin K 1 could not inhibit the differentiation of preadipocytes in vitro . In our study, we have shown that vitamin K 2 but not vitamin K 1 suppressed adipogenesis of SVF cells.…”

Retinoic acid modulates lipid accumulation glucose concentration dependently through inverse regulation of SREBP‐1 expression in 3T3L1 adipocytes

“…In the presence of RA, 3T3-L1 cells cultured in 5.5 mM glucose accumulated lipids less than those cultured in the absence of RA, accompanied with decreases in the mRNA expression of FAS and aP2 ( Fig. 1A-D), indicating that RA suppressed the adipogenic differentiation of mouse 3T3L1 preadipocytes cultured in normal glucose medium as previously reported (Kawada et al 1990;Schwarz et al 1997). Surprisingly, the cells cultured in 25 mM glucose in the presence of RA enhanced lipid accumulation more than those cultured in the absence of RA, accompanied with increases in the mRNA expression of FAS, ACC1a and aP2 ( Fig.…”

Controlled conversion of an immortalized mesodermal progenitor cell towards osteogenic, chondrogenic, or adipogenic pathways.

“…When AA (50 ixg/ml) is added to Dex-treated aggregates, the nodules becomes larger and more adherent but the kinetics of chondrogenic differentiation is not significantly reduced and clusters of adipocytes reproducibly appear within 25 d at the periphery of the spreading aggregates. This effect of AA, as a potent activator of adipose cell differentiation was previously observed (Ono et al, 1990;Kawada et al, 1990).…”

The Vitamin K Epoxide Reductase Vkorc1l1 Promotes Preadipocyte Differentiation in Mice

“…Consistent with our data, rats fed with either extra vitamin K 1 or K 2 displayed decreased body fat and serum TG . Considering that local tissues are likely capable of converting vitamin K 1 to vitamin K 2 in animals , these results support that vitamin K 2 plays the major role in impeding adipogenesis in mammals . As we have shown, vitamin K 2 could suppress the expression of Pparg2 , which is a key regulator in promoting adipogenesis (Supporting Information Figure S5A).…”

“…A previous report showed that vitamin K 1 could not inhibit the differentiation of preadipocytes in vitro . In our study, we have shown that vitamin K 2 but not vitamin K 1 suppressed adipogenesis of SVF cells.…”

Retinoic acid modulates lipid accumulation glucose concentration dependently through inverse regulation of SREBP‐1 expression in 3T3L1 adipocytes

“…In the presence of RA, 3T3-L1 cells cultured in 5.5 mM glucose accumulated lipids less than those cultured in the absence of RA, accompanied with decreases in the mRNA expression of FAS and aP2 ( Fig. 1A-D), indicating that RA suppressed the adipogenic differentiation of mouse 3T3L1 preadipocytes cultured in normal glucose medium as previously reported (Kawada et al 1990;Schwarz et al 1997). Surprisingly, the cells cultured in 25 mM glucose in the presence of RA enhanced lipid accumulation more than those cultured in the absence of RA, accompanied with increases in the mRNA expression of FAS, ACC1a and aP2 ( Fig.…”

Controlled conversion of an immortalized mesodermal progenitor cell towards osteogenic, chondrogenic, or adipogenic pathways.

“…When AA (50 ixg/ml) is added to Dex-treated aggregates, the nodules becomes larger and more adherent but the kinetics of chondrogenic differentiation is not significantly reduced and clusters of adipocytes reproducibly appear within 25 d at the periphery of the spreading aggregates. This effect of AA, as a potent activator of adipose cell differentiation was previously observed (Ono et al, 1990;Kawada et al, 1990).…”