Comparative genomics of Fructobacillus spp. and Leuconostoc spp. reveals niche-specific evolution of Fructobacillus spp.

Endo, Akihito; Tanizawa, Yasuhiro; Tanaka, Naoto; Maeno, Shintaro; Kumar, Himanshu; Shiwa, Yuh; Okada, Sanae; Yoshikawa, Hideki; Dicks, Leon M. T.; Nakagawa, Junichi; Arita, Masanori

doi:10.1186/s12864-015-2339-x

Cited by 59 publications

(80 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…plantarum genome could be related to the diversity of the environmental habitats of this bacterium, while hbs-LAB have evolved in a nutritionally rich environment [22,23]. The characteristic of a small genome and the shift to a nutrient-rich lifestyle has been reported also in Fructobacillus species [44]. Moreover, there is a duplication of genes involved in transport and metabolism of carbohydrates enhancing the ability of LAB [41] and hbs-LAB [22] to exploit sugar-rich environments.…”

Section: Discussionmentioning

confidence: 99%

Improvement of identification methods for honeybee specific Lactic Acid Bacteria; future approaches

Lamei

Olofsson

et al. 2017

PLoS ONE

View full text Add to dashboard Cite

Honeybees face many parasites and pathogens and consequently rely on a diverse set of individual and group-level defenses to prevent disease. The crop microbiota of Apis mellifera, composed of 13 Lactic Acid Bacterial (LAB) species within the genera Lactobacillus and Bifidobacterium, form a beneficial symbiotic relationship with each other and the honeybee to protect their niche and their host. Possibly playing a vital role in honeybee health, it is important that these honeybee specific Lactic Acid Bacterial (hbs-LAB) symbionts can be correctly identified, isolated and cultured, to further investigate their health promoting properties. We have previously reported successful identification to the strain level by culture-dependent methods and we recently sequenced and annotated the genomes of the 13 hbs-LAB. However, the hitherto applied techniques are unfortunately very time consuming, expensive and not ideal when analyzing a vast quantity of samples. In addition, other researchers have constantly failed to identify the 13 hbs-LAB from honeybee samples by using inadequate media and/or molecular techniques based on 16S rRNA gene sequencing with insufficient discriminatory power. The aim of this study was to develop better and more suitable methods for the identification and cultivation of hbs-LAB. We compared currently used bacterial cultivation media and could for the first time demonstrate a significant variation in the hbs-LAB basic requirements for optimal growth. We also present a new bacterial identification approach based on amplicon sequencing of a region of the 16S rRNA gene using the Illumina platform and an error correction software that can be used to successfully differentiate and rapidly identify the 13 hbs-LAB to the strain level.

show abstract

Section: Discussionmentioning

confidence: 99%

Improvement of identification methods for honeybee specific Lactic Acid Bacteria; future approaches

Lamei

Olofsson

et al. 2017

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…To date, the literature reports that just a few carbohydrates are fermented by FLAB (4) and that Fructobacillus spp. lack pentose and glucuronate interconversions and do not metabolize mannose, galactose, starch, sucrose, amino sugars, or nucleotide sugars (8). Nevertheless, L. kunkeei and F. fructosus isolated from the honeybee's gut represent a relatively heterogeneous group, as they have been shown to comprise many plasmids with high diversity among different strains (53,55).…”

Section: Discussionmentioning

confidence: 99%

“…ructophilic lactic acid bacteria (FLAB), described only recently (1)(2)(3)(4)(5)(6)(7)(8)(9), belong to a special group of lactic acid bacteria (LAB) that prefer fructose instead of glucose as a carbon source. These bacteria have been isolated from specific ecological fructose-rich niches such as flowers, fruits, and fermented food based-fruits and, only recently, from the gastrointestinal tracts (GITs) of several insects (e.g., bumblebees, honeybees, tropical fruit flies, and Camponotus ants), which have a fructose-based diet (4,10,11).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Metabolism of Fructophilic Lactic Acid Bacteria Isolated from the Apis mellifera L. Bee Gut: Phenolic Acids as External Electron Acceptors

Filannino

Cagno

Addante

et al. 2016

Appl Environ Microbiol

View full text Add to dashboard Cite

Fructophilic lactic acid bacteria (FLAB) are strongly associated with the gastrointestinal tracts (GITs) of Apis mellifera L. worker bees due to the consumption of fructose as a major carbohydrate. Seventy-seven presumptive lactic acid bacteria (LAB) were isolated from GITs of healthy A. mellifera L. adults, which were collected from 5 different geographical locations of the Apulia region of Italy. Almost all of the isolates showed fructophilic tendencies: these isolates were identified as Lactobacillus kunkeei (69%) or Fructobacillus fructosus (31%). A high-throughput phenotypic microarray targeting 190 carbon sources was used to determine that 83 compounds were differentially consumed. Phenotyping grouped the strains into two clusters, reflecting growth performance. The utilization of phenolic acids, such as p-coumaric, caffeic, syringic, or gallic acids, as electron acceptors was investigated in fructose-based medium. Almost all FLAB strains showed tolerance to high phenolic acid concentrations. p-Coumaric acid and caffeic acid were consumed by all FLAB strains through reductases or decarboxylases. Syringic and gallic acids were partially metabolized. The data collected suggest that FLAB require external electron acceptors to regenerate NADH. The use of phenolic acids as external electron acceptors by the 4 FLAB showing the highest phenolic acid reductase activity was investigated in glucose-based medium supplemented with p-coumaric acid. Metabolic responses observed through a phenotypic microarray suggested that FLAB may use p-coumaric acid as an external electron acceptor, enhancing glucose dissimilation but less efficiently than other external acceptors such as fructose or pyruvic acid. Fructophilic lactic acid bacteria (FLAB), described only recently (1-9), belong to a special group of lactic acid bacteria (LAB) that prefer fructose instead of glucose as a carbon source. These bacteria have been isolated from specific ecological fructose-rich niches such as flowers, fruits, and fermented food based-fruits and, only recently, from the gastrointestinal tracts (GITs) of several insects (e.g., bumblebees, honeybees, tropical fruit flies, and Camponotus ants), which have a fructose-based diet (4, 10, 11). Among these insects, social honeybees are those of greater interest because of their economic and ecological importance for honeybee products (e.g., propolis, royal jelly, honey, and pollen) and especially for crop pollination. Despite a global increase in the population of domesticated bees according to the FAO data (12), honeybees are facing growing adversity. Localized declines in bee populations have occurred in many European countries (13-15). To understand and to prevent the decrease, several studies have been addressed to investigate the symbiotic and pathogenic microbial interactions (16-19). The observations made as of to date indicate the honeybees' GITs harbor a core microbiota dissimilar to those of other animals, including humans (16)(17)(18)(19)(20)(21)(22)(23)(24). FLAB are strongly as...

show abstract

“…Fructobacillus is another plant‐associated LAB genus for which only a limited amount of information is available (Table ). Fructobacillus is comprised of five species ( F. fructosus , F. durionis , F. ficulneus , F. pseudoficulneus and F. tropaeoli ) and has only been isolated from fructose‐rich environments (flowers, fruits and bee digestive tracts) (Endo et al ., ; ). Species of Fructobacillus and several species of Lactobacillus ( L. apinorum , L kunkeei and L. florum ) are categorized as fructophilic LAB (FLAB), which are known for their fructophilic as opposed to glucophilic metabolism (Endo et al ., ; ; Tyler et al ., ).…”

Section: Plant‐associated Lab Speciesmentioning

confidence: 99%

Abundance, diversity and plant‐specific adaptations of plant‐associated lactic acid bacteria

Leveau

Marco

2019

Environ Microbiol Rep

View full text Add to dashboard Cite

Summary Lactic acid bacteria (LAB) are essential for many fruit, vegetable and grain food and beverage fermentations. However, the numbers, diversity and plant‐specific adaptions of LAB found on plant tissues prior to the start of those fermentations are not well understood. When measured, these bacteria have been recovered from the aerial surfaces of plants in a range from <10 CFU g−1 to over 108.5 CFU g−1 of plant tissue and in lower quantities from the soil and rhizosphere. Plant‐associated LAB include well‐known generalist taxa such as Lactobacillus plantarum and Leuconostoc mesenteroides, which are essential for numerous food and beverage fermentations. Other plant‐associated LAB encompass specialist taxa such as Lactobacillus florum and Fructobacillus, many of which were discovered relatively recently and their significance on plants and in foods is not yet recognized. LAB recovered from plants possess the capacity to consume plant sugars, detoxify phenolic compounds and tolerate the numerous biotic and abiotic stresses common to plant surfaces. Although most generalist and some specialist LAB grow rapidly in food and beverages fermentations and can cause spoilage of fresh and fermented fruits and vegetables, the importance of living plants as habitats for these bacteria and LAB contributions to plant microbiomes remain to be shown.

show abstract

Comparative genomics of Fructobacillus spp. and Leuconostoc spp. reveals niche-specific evolution of Fructobacillus spp.

Cited by 59 publications

References 42 publications

Improvement of identification methods for honeybee specific Lactic Acid Bacteria; future approaches

Improvement of identification methods for honeybee specific Lactic Acid Bacteria; future approaches

Metabolism of Fructophilic Lactic Acid Bacteria Isolated from the Apis mellifera L. Bee Gut: Phenolic Acids as External Electron Acceptors

Abundance, diversity and plant‐specific adaptations of plant‐associated lactic acid bacteria

Contact Info

Product

Resources

About