Comparative Gene Expression Analysis of Susceptible and Resistant Near-Isogenic Lines in Common Wheat Infected by Puccinia triticina

Abstract

Gene expression after leaf rust infection was compared in near-isogenic wheat lines differing in the Lr10 leaf rust resistance gene. RNA from susceptible and resistant plants was used for cDNA library construction. In total, 55 008 ESTs were sequenced from the two libraries, then combined and assembled into 14 268 unigenes for further analysis. Of these ESTs, 89% encoded proteins similar to (E value of ≤10−5) characterized or annotated proteins from the NCBI non-redundant database representing diverse molecular functions, cellular localization and biological processes based on gene ontology classification. Further, the unigenes were classified into susceptible and resistant classes based on the EST members assembled from the respective libraries. Several genes from the resistant sample (14-3-3 protein, wali5 protein, actin-depolymerization factor and ADP-ribosylation factor) and the susceptible sample (brown plant hopper resistance protein, caffeic acid O-methyltransferase, pathogenesis-related protein and senescence-associated protein) were selected and their differential expression in the resistant and susceptible samples collected at different time points after leaf rust infection was confirmed by RT–PCR analysis. The molecular pathogenicity of leaf rust in wheat was studied and the EST data generated made a foundation for future studies.

Comparative Temporal Transcriptome Profiling of Wheat near Isogenic Line Carrying Lr57 under Compatible and Incompatible Interactions

“…A number of studies have used ESTs (Manickavelu et al, 2010; Dmochowska-Boguta et al, 2015), microarrays (Fofana et al, 2007), serial analysis of gene expression (Chandra et al, 2016), and RNAseq (Zhang et al, 2014; Dobon et al, 2016) to understand the host pathogen relationship between known host genes and specific pathotypes under compatible as well as incompatible interactions.…”

The Soybean Rhg1 Locus for Resistance to the Soybean Cyst Nematode Heterodera glycines Regulates the Expression of a Large Number of Stress- and Defense-Related Genes in Degenerating Feeding Cells

“…Consequently, the use of NILs for molecular studies is becoming more prevalent. NILs have been used in a microarray analysis of iron-efficient and -inefficient cultivars of soybean (O'Rourke et al, 2009) and a wheat (Triticum aestivum) leaf rust resistance gene, Lr10 (Manickavelu et al, 2010). NILs also recently helped to identify the effects of the Arabidopsis (Arabidopsis thaliana) gene FLC on seed germination (Chiang et al, 2009).…”

Stage-specific reprogramming of gene expression characterizes Lr48-mediated adult plant leaf rust resistance in wheat

“…Inactive 'poly (ADP-ribose) polymerase RCD1-like' gene is known to regulate oxidative stress genes (as positive regulator) and several other stress responsive genes (by interacting with transcription factors), and participate in abscisic acid, ethylene, and jasmonate signaling pathways in Arabidopsis (Overmyer et al 2000(Overmyer et al , 2005Ahlfors et al 2004;Fujibe et al 2004;Jaspers et al 2009;Teotia et al 2010;Teotia and Lamb 2011). Expression pattern of this gene, and the association of other genes with Lr48-mediated APR expression, also suggests that different stress-signaling pathways were cross-talking with each other, which may contribute to disease resistance (Manickavelu et al 2010).…”

“…The approaches used for this purpose included cDNA-amplified fragment length polymorphism (cDNA-AFLP), microarray analyses, and development of ESTs (Zhang et al 2003;Fofana et al 2007;Hulbert et al 2007;Bolton et al 2008;Manickavelu et al 2010;Dhariwal et al 2011;Spielmeyer et al 2013;Kumar et al 2014). However, not many gene expression studies involving APR Lr genes in wheat have been conducted; in particular, gene Lr48 has never been studied in this manner.…”