Comparative expression analysis of the genes encoding polypyrimidine tract binding protein (PTB) and its neural homologue (brPTB) in prenatal and postnatal mouse brain

Abstract

The polypyrimidine tract binding protein (PTB) and its recently discovered homologue brain-enriched PTB (brPTB) are RNA binding proteins involved in the control of alternative splicing. We have characterized expression patterns of the PTB and brPTB in course of mouse brain development, using mRNA in situ hybridization. PTB is expressed in choroid plexi and ependyma at all the stages of development and temporarily in the mantle layer of migrating neuroblasts of fore-, mid- and hindbrain and in the external granular layer of cerebellum. In the neurons of adult mouse cerebrum and cerebellum expression of PTB is undetectable. In contrast to this, brPTB is expressed ubiquitously in neuroblasts of various parts of embryonic brain and in the differentiated neurons of postnatal cerebrum and cerebellum. brPTB mRNA is not observed in choroid plexi and ependymal layer. Thus, in the embryonic brain expression patterns of PTB and brPTB overlap, but in the course of brain development the patterns become complementary to each other.

Expression analysis of the polypyrimidine tract binding protein (PTBP1) and its paralogs PTBP2 and PTBP3 during Xenopus tropicalis embryogenesis

“…3 B,C panels a-c) suggesting that most of the maternal functions of this RBP family are performed by PTBP1. Consistent with previous studies in mouse (Lillevali et al, 2001), the expression of ptbp2 coincided with the formation of the nervous system and ptbp2 neural plate of late neurula stage (stage 18, Fig. 3B panel d and form the brain.…”

Polypyrimidine tract binding protein and Notch1 are independently re-expressed in glioma

“…However, in the adult mouse brain, PTB is absent in mature astrocytes and occurs at low levels in neural progenitors of the choroid plexus and in the ependymal regions near the ventricles. 13 In a similar manner, the adult human brain has little or no PTB expression in normal astrocytes and oligodendrocytes, with low expression in ependymocytes, the choroid plexus epithelium, microglia, the arachnoid, and the adenohypophysis. 14 Resurgent expression of PTB has been observed in astrocyte-derived gliomas that is analogous to the expression of other tumor-associated developmental markers.…”

MicroRNAs regulate the expression of the alternative splicing factor nPTB during muscle development

“…Polypyrimidine tract-binding protein (PTB/hnRNPI/PTBP1) is a well-studied splicing regulator that is expressed in a wide range of tissues (Garcia-Blanco et al 1989;Patton et al 1991;Ghetti et al 1992;Wagner and Garcia-Blanco 2001;Spellman and Smith 2006). At least two additional PTB homologs exist in mammals: ROD1, which is restricted to hematopoetic cells (Yamamoto et al 1999), and nPTB/brPTB/PTBP2, which is expressed primarily in neurons and testes and at lower levels in muscle (Ashiya and Grabowski 1997;Chan and Black 1997;Irwin et al 1997;Kikuchi et al 2000;Markovtsov et al 2000;Polydorides et al 2000;Lillevali et al 2001). Rodents also contain a smooth muscle expressed homolog, smPTB (Gooding et al 2003).…”