Comparative evaluation of two different methods of inoculum preparation for antifungal susceptibility testing of filamentous fungi

Aberkane, Amel; Cuenca‐Estrella, Manuel; Gómez-López, Alicia; Petrikkou, Evangelia; Mellado, Emilia; Monzón, Araceli; Rodríguez-Tudela, Juan Luis

doi:10.1093/jac/dkf187

Cited by 101 publications

(73 citation statements)

References 9 publications

(11 reference statements)

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“…Fungal strains were maintained on Potato Dextrose (PD) agar. Haemocytometer cell-counting, with some modifications was used for counting the number of cells for each fungal culture (Aberkane et al, 2002). The inoculum of each isolate was prepared by first growing the fungus on potato dextrose (PD) agar slants for seven days at 35°C.…”

Section: Fungal Strains and Inoculum Quantificationmentioning

confidence: 99%

Antioxidant and Antifungal Activity of Selected Medicinal Plant Extracts Against Phytopathogenic Fungi.

Mahlo¹,

Chauke²,

McGaw³

et al. 2016

Afr J Tradit Complement Altern Med

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Background: Medicinal plants are used by many ethnic groups as a source of medicine for the treatment of various ailments in both humans and domestic animals. These plants produce secondary metabolites that have antimicrobial properties, thus screening of medicinal plants provide another alternative for producing chemical fungicides that are relatively non-toxic and cost-effective. Materials and methods: Leaf extracts of selected South African plant species (Bucida buceras, Breonadia salicina, Harpephyllum caffrum, Olinia ventosa, Vangueria infausta and Xylotheca kraussiana) were investigated for activity against selected phytopathogenic fungi (Aspergillus niger, Aspergillus parasiticus, Colletotricum gloeosporioides, Penicillium janthinellum, P. expansum, Trichoderma harzianum and Fusarium oxysporum). These plant fungal pathogens causes major economic losses in fruit industry such as blue rot on nectaries and postharvest disease in citrus. Plant species were selected from 600 evaluated inter alia, against two animal fungal pathogens (Candida albicans and Cryptococcus neoformans). Antioxidant activity of the selected plant extracts were investigated using a qualitative assay (2, 2-diphenyl-1-picrylhydrazyl (DPPH)). Bioautography assay was used to determine the number of antifungal compounds in plant extracts. Results: All plant extracts were active against the selected plant phytopathogenic fungi. Moreover, Bucida buceras had the best antifungal activity against four of the fungi, with minimum inhibitory concentration (MIC) values as low as 0.02 mg/ml and 0.08 mg/ml against P. expansum, P. janthinellum, T. harzianum and F. oxysporum. The plant extracts of five plant species did not possess strong antioxidant activity. However, methanol extract of X. kraussiana was the most active radical scavenger in the DPPH assay amongst the six medicinal plants screened. No antifungal compounds were observed in some of the plant extracts with good antifungal activity as shown in the microdilution assay, indicating possible synergism between the separated metabolites. Conclusion: The results showed that acetone was the best extractant. Furthermore, our findings also confirm the traditional use of Breonadia salicina and demonstrate the potential value of developing biopesticides from plants.

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Section: Fungal Strains and Inoculum Quantificationmentioning

confidence: 99%

Antioxidant and Antifungal Activity of Selected Medicinal Plant Extracts Against Phytopathogenic Fungi.

Mahlo¹,

Chauke²,

McGaw³

et al. 2016

Afr J Tradit Complement Altern Med

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“…After appropriate dilutions, inoculum sizes (spores/ mL) were determined by direct microscopic observation using a haemocytometer (Aberkane et al 2002).…”

Section: Inoculum Developmentmentioning

confidence: 99%

Assessment of the Effect of Easily-metabolised Carbon Supplements on Xylanase Production by Newly Isolated Trichoderma asperellum USM SD4 Cultivated on Oil Palm Empty Fruit Bunches

Ajijolakewu¹,

Leh²,

Abdullah³

et al. 2016

BioResources

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“…This standard is similar to the one published by the CLSI (formerly NCCLS) reference method for broth dilution antifungal susceptibility testing of filamentous fungi, but with the following minor modifications: (i) RPMI 1640 was supplemented with glucose to reach a 2% concentration and (ii) the inoculum sizes were between 1.0 ϫ 10 5 and 5.0 ϫ 10 5 CFU/ml (1,3,16,18). The antifungal susceptibility results for the blood isolate were as follows: amphotericin B, 1 g/ml; itraconazole, Ͼ8 g/ml; voriconazole, 4 g/ml; posaconazole, Ͼ8 g/ml; and terbinafine, 2 g/ml.…”

Section: Case Reportmentioning

confidence: 99%

Disseminated Fusariosis Caused by Fusarium verticillioides in an Acute Lymphoblastic Leukemia Patient after Allogeneic Hematopoietic Stem Cell Transplantation

et al. 2009

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Fusarium species are saprophytic molds which cause disseminated or localized infections in humans. Disseminated Fusarium infection can cause significant morbidity and mortality in immunocompromised patients. We present a case of disseminated fusariosis caused by Fusarium verticillioides in a patient with acute lymphoblastic leukemia and successfully treated using both liposomal amphotericin B and voriconazole. CASE REPORTA 12-year-old boy underwent allogeneic hematopoietic stem cell transplant (allo-HSCT) from unrelated cord blood for high-risk acute lymphoblastic leukemia in its first complete remission. The conditioning regimen included busulfex (12.8 mg/kg of body weight), etoposide (40 mg/kg), and cyclophosphamide (120 mg/kg). For graft-versus-host-disease prophylaxis, cyclosporine A was added on day Ϫ1 but switched to mycophenolate mofetil due to severe allergic reaction. By the fourth day after the transplant, he was in severe neutropenia (Ͻ0.1 ϫ 10 3 neutrophils/l) and became febrile, and antibiotic treatment (meropenem) was initiated for febrile neutropenia. Although the fever disappeared within few days of antibiotic onset, on day 23 posttransplant, he again became febrile, and treatment with liposomal amphotericin B (LAmB) was then started, with a dosage of 3 mg/kg/day. Cultures of separate blood samples obtained percutaneously and from a central venous catheter yielded coagulase-negative Staphylococcus epidermidis, and teicoplanin was added. On day 59 posttransplant, the patient developed multiple skin lesions, starting from the extremities and spreading to the face and trunk. The lesions had necrotic centers surrounded by spreading erythema (Fig. 1). A biopsy of the skin lesion showed the presence of histopathological symptoms consistent with a septate pathogenic mold, and blood cultures taken on the same day were positive for a Fusarium species.The diagnosis of disseminated fusariosis was established, and LAmB was raised to a dose of 5 mg/kg/day. During the days following, the lesions worsened and treatment with voriconazole (loading dose, 6 mg/kg/day, followed by 4 mg/kg/day intravenously every 12 h) was initiated. A chest X-ray and a high-resolution computed tomography scan of the lungs were normal. Since profound neutropenia still continued, it was accepted that the patient had graft failure and new, unrelated donor search was started. Since a fully HLA-matched donor was not available, the patient underwent peripheral blood stem cell transplantation from a 9/10-matched, unrelated donor on day 82 posttransplant. The conditioning regimen included cyclophosphamide (120 mg/kg), and for graft-versus-host-disease prophylaxis, antithymocyte globulin (60 mg/kg), mycophenolate mofetil, and methotrexate were used. Neutrophil engraftment occurred on day 13 posttransplant. Combined-antifungal therapy and antifungal therapy with only voriconazole were continued until the ends of first and third months after peripheral blood stem cell transplantation, respectively. Two weeks after discontinuation of voriconaz...

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Comparative evaluation of two different methods of inoculum preparation for antifungal susceptibility testing of filamentous fungi

Cited by 101 publications

References 9 publications

Antioxidant and Antifungal Activity of Selected Medicinal Plant Extracts Against Phytopathogenic Fungi.

Antioxidant and Antifungal Activity of Selected Medicinal Plant Extracts Against Phytopathogenic Fungi.

Assessment of the Effect of Easily-metabolised Carbon Supplements on Xylanase Production by Newly Isolated Trichoderma asperellum USM SD4 Cultivated on Oil Palm Empty Fruit Bunches

Disseminated Fusariosis Caused by Fusarium verticillioides in an Acute Lymphoblastic Leukemia Patient after Allogeneic Hematopoietic Stem Cell Transplantation

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