Journal of Chromatography A 2012 DOI: 10.1016/j.chroma.2012.02.019 View full text
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Raimondas Raudonis, Lina Raudone, Valdas Jakstas, Valdimaras Janulis

Abstract: ABTS and FRAP post-column techniques evaluate the antioxidant characteristics of HPLC separated compounds with specific reagents. ABTS characterize their ability to scavenge free radicals by electron-donating antioxidants, resulting in the absorbance decrease of the chromophoric radical. FRAP - is based on the reduction of Fe(III)-tripyridyltriazine complex to Fe(II)-tripyridyltriazine at low pH by electron-donating antioxidants, resulting in an absorbance increase. Both post-column assays were evaluated and c…

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