Comparative evaluation of PLGA nanoparticle delivery system for 5-fluorouracil and curcumin on squamous cell carcinoma

Masloub, Shaimaa; Elmalahy, Mohamed H.; Sabry, Dina; Mohamed, Wael S.; Ahmed, Sahar

doi:10.1016/j.archoralbio.2015.12.003

Cited by 36 publications

(15 citation statements)

References 40 publications

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“…One of the most widely used DDSs is the biodegradable and biocompatible copolymer poly (d,l-lactic-co-glycolic acid) (PLGA) due to its metabolite monomers: Lactic acid and glycolic acid. This polymer is approved by the Food and Drug Administration (FDA) and the European Medicine Agency (EMA) in several clinically applied DDS [15]. Nanoparticles (NPs) are a form of DDS.…”

Section: Introductionmentioning

confidence: 99%

Effect of Oxaliplatin-Loaded Poly (d,l-Lactide-co-Glycolic Acid) (PLGA) Nanoparticles Combined with Retinoic Acid and Cholesterol on Apoptosis, Drug Resistance, and Metastasis Factors of Colorectal Cancer

et al. 2020

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Apoptosis signaling pathways, drug resistance, and metastasis are important targets to develop new cancer treatments. We developed cholesterol-coated Poly(d,l-Lactide-co-Glycolic Acid) (PLGA) nanoparticles for effective encapsulation and delivery of retinoic acid and oxaliplatin to analyze their antitumor activity in colorectal cancer. The cell viability and proliferation of tumoral cells lines (CT-26 and SW-480) decreased when compared to control in vitro after treatment with the nanoparticles. In addition, apoptosis of CT-26 cells increased. Importantly, cytoprotection of nontumor cells was detected. Expression of pro-apoptotic proteins was upregulated, while anti-apoptotic proteins were downregulated either in vitro or in vivo. In addition, drug resistance and metastasis factors were downregulated in vivo. Human colorectal tumors that highly expressed BCL-2 and Ki-67 had a greater tendency towards death within 60 months. Our results show that loading oxaliplatin combined with retinoic acid and cholesterol in a nanoparticle formulation enables determination of optimal antitumor activity and subsequent treatment efficacy.

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Section: Introductionmentioning

confidence: 99%

Effect of Oxaliplatin-Loaded Poly (d,l-Lactide-co-Glycolic Acid) (PLGA) Nanoparticles Combined with Retinoic Acid and Cholesterol on Apoptosis, Drug Resistance, and Metastasis Factors of Colorectal Cancer

et al. 2020

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“…However, the usage of curcumin is limited by the poor bioavailability due to its β -diketonate structure [13]. Although novel biocompatible nanosystems for curcumin delivery have been developed, the anticancer effects of curcumin nanoparticles could not last for a long time [21, 22]. The β -diketonate structure in curcumin is modified in MCACs (Figure 1(a)), which could improve its chemical stability in a different way [23].…”

Section: Discussionmentioning

confidence: 99%

Curcumin Analogue CA15 Exhibits Anticancer Effects on HEp-2 Cells via Targeting NF-κB

Chen

Zhang

Shu

et al. 2017

BioMed Research International

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Laryngeal carcinoma remains one of the most common malignancies, and curcumin has been proven to be effective against head and neck cancers in vitro. However, it has not yet been applied in clinical settings due to its low stability. In the current study, we synthesized 34 monocarbonyl analogues of curcumin with stable structures. CA15, which exhibited a stronger inhibited effect on laryngeal cancer cells HEp-2 but a lower toxicity on hepatic cells HL-7702 in MTT assay, was selected for further analysis. The effects of CA15 on cell viability, proliferation, migration, apoptosis, and NF-κB activation were measured using MTT, Transwell migration, flow cytometry, Western blot, and immunofluorescence assays in HEp-2 cells. An NF-κB inhibitor, BMS-345541, as well as curcumin was also tested. Results showed that CA15 induced decreased toxicity towards HL-7702 cells compared to curcumin and BMS-345541. However, similar to BMS-345541 and curcumin, CA15 not only significantly inhibited proliferation and migration and induced caspase-3-dependent apoptosis but also attenuated TNF-α-induced NF-κB activation in HEp-2 cells. These results demonstrated that curcumin analogue CA15 exhibited anticancer effects on laryngeal cancer cells via targeting of NF-κB.

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“…A standard water‐in‐oil‐in‐water (w/o/w) double emulsion solvent evaporation method was used for preparation of the 5‐FU‐loaded PLGA microspheres . 5‐FU (30 mg) was dissolved in deionized water.…”

Section: Methodsmentioning

confidence: 99%

“…X‐ray diffraction and differential scanning calorimetry techniques were also used to understand the thermal behavior of PLGA microsphere . These techniques can provide insights related to morphology, size, shape, amount of drug loading, and the changes induced in the properties of PLGA due to the presence of the drug . However, information related to interior of the microsphere, local organization of entrapped molecules, and possible intermolecular interactions within the cavity of such systems are rarely highlighted by the previously mentioned methods.…”

Section: Introductionmentioning

confidence: 99%

Solution dynamics of 5‐fluorouracil entrapped in poly lactic‐co‐glycolic acid (PLGA) microsphere—A study with 1D selective NMR methods

Kumar

Pal

2018

Magnetic Reson in Chemistry

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In this report, our main focus is to introduce a set of one‐dimensional (1D) NMR methods based on chemical shift, relaxation, and magnetization transfer, namely, NOE and chemical exchange involving selective pulse excitation to study the solution dynamics of drug in free and encapsulated state within polymeric microsphere. In this regard 5‐fluorouracil (5‐FU) loaded poly lactic‐co‐glycolic acid (PLGA) microspheres are prepared as model system via standard water‐in‐oil‐in‐water emulsification method. One‐dimensional 1H and 19F nuclear magnetic resonance (NMR) spectra of 5‐FU in presence of PLGA microspheres presented a significant change in linewidth and relaxation rates compared with free 5‐FU confirming encapsulation. Furthermore, loss of coupling pattern in 1H and 19F NMR of PLGA encapsulated 5‐FU as compared with free 5‐FU suggests an enhanced –NH and –H2O protons exchange dynamics in the interior of the microsphere indicating hydrated microsphere cavity. Quantification of exchange dynamics in case of free and PLGA‐encapsulated 5‐FU was attempted employing 1D selective NOESY and 1D multiply selective inversion recovery experiments. Analysis of the exchange rates confirmed existence of more than one kind of water population within the cavity as mentioned in an earlier solid state NMR report.

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Comparative evaluation of PLGA nanoparticle delivery system for 5-fluorouracil and curcumin on squamous cell carcinoma

Cited by 36 publications

References 40 publications

Effect of Oxaliplatin-Loaded Poly (d,l-Lactide-co-Glycolic Acid) (PLGA) Nanoparticles Combined with Retinoic Acid and Cholesterol on Apoptosis, Drug Resistance, and Metastasis Factors of Colorectal Cancer

Effect of Oxaliplatin-Loaded Poly (d,l-Lactide-co-Glycolic Acid) (PLGA) Nanoparticles Combined with Retinoic Acid and Cholesterol on Apoptosis, Drug Resistance, and Metastasis Factors of Colorectal Cancer

Curcumin Analogue CA15 Exhibits Anticancer Effects on HEp-2 Cells via Targeting NF-κB

Solution dynamics of 5‐fluorouracil entrapped in poly lactic‐co‐glycolic acid (PLGA) microsphere—A study with 1D selective NMR methods

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