2017
DOI: 10.18535/ijsrm/v5i3.12
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Abstract: and The holding company for the production of Vaccines , Sera and Drugs (VACSERA). AbstractThe present work aimed to evaluate the anticancer potentials concerning the cytotoxicity and antiproliferative activity of L-amino acid Oxidase (LAAO) ,MoringaOleifera(MO) Methanol (MLM) and water extracts (MLW), Ganodermalucidum(GL), extracts as a natural products and Silver nanoparticles (AgNP) as well. Cytotoxic effect of test extracts on both breast (MCF-7) and liver(HEPG-2) cancer cell lines varied according to test… Show more

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“…RNA was extracted from each sample treated cell lines using nuclease free-water [42] and stored at − 70°C. Concentration and purity of the extracted RNA were determined according to manufacturer's protocol, where RNA was diluted with distilled water and the optical density was measured spectrophotometrically at 260 and 280 nm and the expected absorbance range of pure extracted RNA should be within 1.7 to 2.1 according to [43] For each sample, extracted RNA (1 μg), random hexamer primer (1 μl) and DEPC-treated water (to12 μl) were mixed, centrifuged briefly and incubated at 65°C for 5 min.…”
Section: Gene Expressionmentioning
confidence: 99%
“…RNA was extracted from each sample treated cell lines using nuclease free-water [42] and stored at − 70°C. Concentration and purity of the extracted RNA were determined according to manufacturer's protocol, where RNA was diluted with distilled water and the optical density was measured spectrophotometrically at 260 and 280 nm and the expected absorbance range of pure extracted RNA should be within 1.7 to 2.1 according to [43] For each sample, extracted RNA (1 μg), random hexamer primer (1 μl) and DEPC-treated water (to12 μl) were mixed, centrifuged briefly and incubated at 65°C for 5 min.…”
Section: Gene Expressionmentioning
confidence: 99%