Comparative Evaluation of Anthelmintic Activity of Edible and Ornamental Pomegranate Ethanolic Extracts againstSchistosoma mansoni

Abstract

Due to the development of praziquantel (PZQ) schistosomes resistant strains, the discovery of new antischistosomal agents is of high priority in research. This work reported the in vitro and in vivo effects of the edible and ornamental pomegranate extracts against Schistosoma mansoni. Leaves and stem bark ethanolic extracts of both dried pomegranates were prepared at 100, 300, and 500 μg/mL for in vitro and 600 and 800 mg/kg for in vivo. Adult worms Schistosoma mansoni in RPMI-1640 medium for in vitro and S. mansoni infected mice for in vivo tests were obtained from Theodor Bilharz Research Institute, Cairo, Egypt. In vitro activity was manifested by significant coupled worms separation, reduction of motor activity, lethality, and ultrastructural tegumental alterations in adult worms. In vivo activity was manifested revealed by significant reduction of hepatic granulomas number and diameter, decreased number of bilharzial eggs in liver tissues, lowered liver inflammatory infiltration, decreased hepatic fibrosis, and inducible nitric oxide synthase (iNOS) expression. Ethanolic stem bark extract of edible pomegranate exhibited highest antischistosomal activities both in vitro and in vivo. Therefore, pomegranate showed a good potential to be used as a promising new candidate for the development of new schistosomicidal agents.

In vitro and in vivo activities of DW-3-15, a commercial praziquantel derivative, against Schistosoma japonicum

“…In addition, there was a significant reduction in the average diameter of hepatic granuloma in mice treated with DW-3-15, indicating the anti-inflammatory effect of DW-3-15. Similar results have been reported for other compounds, including 8-hydroxyquinoline derivatives and pomegranate extract [41, 42]. Earlier studies reported that the reduction in granuloma diameter was significantly related to egg burden eliminated in the feces [43].…”

Berberina, curcumina y quercetina como potenciales agentes con capacidad antiparasitaria

“…Existe un renovado interés en los productos naturales como punto de partida para el descubrimiento de medicamentos para combatir las parasitosis, ya que son escasos los recursos para tratarlas (Yones et al, 2016). Por lo cual es necesario desarrollar nuevos medicamentos contra estas enfermedades que afectan al hombre mundialmente.…”

<p>In vitro Effects of <em>Punica granatum</em> Ellagitannins on Adult Worms of <em>Schistosoma mansoni</em></p>

“…Tegumental changes were previously observed in adult worms treated with crude alcoholic extracts of the stem bark, rind and leaf of pomegranate. 20 , 33 Crude extracts of pomegranate leaf and stem bark caused separation of the worm couples and decreased worm’s motility. 20 Our data suggest that these reported tegumental changes and the separation of worm couples are probably, at least in part, due to the ellagitannins of the pomegranate.…”

“…This might be behind the uncoupling of worms which was observed in this study and was also caused by crude pomegranate extracts in another study. 20 …”

“…19 The P. granatum extracts was significantly found to reduced the number and diameter of hepatic granulomas, decrease the number of schistosomal eggs in liver tissues, lowerthe liver inflammatory infiltration, and decrease the hepatic fibrosis in mice. 20 …”