Comparative Efficacies of a 3D-Printed PCL/PLGA/β-TCP Membrane and a Titanium Membrane for Guided Bone Regeneration in Beagle Dogs

Abstract

This study was conducted to evaluate the effects of a 3D-printed resorbable polycaprolactone/poly(lactic-co-glycolic acid)/β-tricalcium phosphate (PCL/PLGA/β-TCP) membrane on bone regeneration and osseointegration in areas surrounding implants and to compare results with those of a non-resorbable titanium mesh membrane. After preparation of PCL/PLGA/β-TCP membranes using extrusion-based 3D printing technology; mechanical tensile testing and in vitro cell proliferation testing were performed. Implant surgery and guided bone regeneration were performed randomly in three groups (a no membrane group, a titanium membrane group, and a PCL/PLGA/β-TCP membrane group (n = 8 per group)). Histological and histometric analyses were conducted to evaluate effects on bone regeneration and osseointegration. Using the results of mechanical testing; a PCL/PLGA/β-TCP ratio of 2:6:2 was selected. The new bone areas (%) in buccal defects around implants were highest in the PCL/PLGA/β-TCP group and significantly higher than in the control group (p < 0.05). Bone-to-implant contact ratios (%) were also significantly higher in the PCL/PLGA/β-TCP and titanium groups than in the control group (p < 0.05). When the guided bone regeneration procedure was performed using the PCL/PLGA/β-TCP membrane; new bone formation around the implant and osseointegration were not inferior to those of the non-resorbable pre-formed titanium mesh membrane.

Comparative Efficacies of Collagen-Based 3D Printed PCL/PLGA/β-TCP Composite Block Bone Grafts and Biphasic Calcium Phosphate Bone Substitute for Bone Regeneration

“…Stiffness is increased with an increase in PLGA ratio [27,28]. In the previous study in which the barrier membrane was fabricated with PCL/PLGA/β-TCP at a ratio of 2:6:2, the stiffness was similar to that of titanium membrane [27,28].…”

Effect of enamel matrix derivative liquid in combination with a natural bone mineral on new bone formation in a rabbit GBR model

“…The following three treatment modalities were randomly allocated to all 24 defects: (a) control (empty) ( n = 8), (b) 20 mg of NBM particles (NBM; Bio‐Oss ® , S‐size, Geistlich Pharma AG) ( n = 8), and (c) 20 mg of NBM + 20 µl of EMD‐liquid ( n = 8). The sample size was chosen based on previous studies (de Brito Bezerra et al, ; Findikcioglu, Findikcioglu, Yavuzer, Elmas, & Atabay, ; Fujioka‐Kobayashi, Kobayashi, et al, ; Shim et al, ). Bilayer collagen barrier membranes (BioGide ® , Geistlich Pharma AG) in 8‐mm diameter circle was utilized to cover all defects (each group) with the rough side facing the bone defect, followed by the periosteum and skin closure with interrupted sutures in layers using 3‐0 Vicryl and 4‐0 Nylon sutures.…”

Polylactic Acid Based Nanocomposites: Promising Safe and Biodegradable Materials in Biomedical Field

“…The appearance of a new method called 3D printing based on computer-aided design (CAD) can perfectly solve this intractable problem and it has led to a revolution in manufacturing industry. Shim et al [81] recently creatively prepared a resorbable semi-dome-shaped polycaprolactone (PCL)/PLGA/ -TCP membrane through a 3D printing system (Figure 4), with both rapid degradation rate of PLGA and high elasticity of PCL taken into consideration. In vitro mechanical and cytology test, in vivo preclinical implanting experiment, and a comparison with a titanium membrane were all conducted.…”

“…When bone regeneration experiment was performed on oral bone defects, results showed the PCL/PLGA/ -TCP membrane had almost similar properties to those nonresorbable and commonly used titanium mesh membrane, such as osseointegration and the ability to form 6 International Journal of Polymer Science Figure 4: A PCL/PLGA/ -TCP membrane produced using 3D printing technology. Pores were triangularly structured and completely interconnected (originally adapted from [81]). new bone around the implants.…”