Comparative Effects of Vaporized Perfluorohexane and Partial Liquid Ventilation in Oleic Acid– induced Lung Injury

Abreu, Marcelo Gama de; Quelhas, André Domingues; Spieth, Peter M.; Bräuer, G.; Knels, Lilla; Kasper, Michael; Pino, Alexandre Visintainer; Bleyl, Jörg-Uwe; Hübler, Matthias; Bozza, Fernando A.; Salluh, Jorge I.; Kuhlisch, Eberhard; Giannella-Neto, Antonio; Koch, Thea

doi:10.1097/00000542-200602000-00013

Cited by 31 publications

(22 citation statements)

References 23 publications

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“…Higher PTP values may indicate increased activity of respiratory muscles, which likely contributed to redistribution of ventilation towards dependent regions during PAV. [8][9][10][11][12][13][14] Dependent, gravitational dependent lung regions (dorsal); nondependent, gravitational nondependent lung regions (ventral). a p < .05 vs. PSV; b p < .05 vs. noisy PSV; c p < .05 vs. dependent/nondependent region within the same group, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…Animals were kept in supine position during the entire experiment. Hemodynamics, gas exchange, and respiratory mechanics were obtained at intervals as previously described (14) (see the supplemental data for details, Supplemental Digital Content 1, http://links.lww.com/CCM/A471). Electrical impedance tomography was performed to measure regional distribution of ventilation at the xiphoid level.…”

Section: Preparationmentioning

confidence: 99%

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Comparative effects of proportional assist and variable pressure support ventilation on lung function and damage in experimental lung injury*

Spieth

Güldner

Beda

et al. 2012

Critical Care Medicine

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In a lung lavage model of acute lung injury, both variable pressure support and proportional assist ventilation increased the variability of tidal volume and improved oxygenation and venous admixture, without influencing subject-ventilator synchrony or affecting lung injury compared with pressure support ventilation. However, variable pressure support yielded less inspiratory effort than proportional assist ventilation at comparable mean tidal volumes of 6 mL/kg.

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Section: Discussionmentioning

confidence: 99%

Section: Preparationmentioning

confidence: 99%

Comparative effects of proportional assist and variable pressure support ventilation on lung function and damage in experimental lung injury*

Spieth

Güldner

Beda

et al. 2012

Critical Care Medicine

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“…The pathologic features of the lungs were scored based on alveolar edema, interstitial edema, hemorrhage, and inflammatory infiltration. The scoring system used in this work was adapted and modified from a previous study [21]. In our scoring system, values from 0 to 3 represent the severity of the pathologic feature as follows: 0 ¼ normal appearance, 1 ¼ slight damage in those above four features, 2 ¼ intermediate damage, and 3 ¼ severe damage.…”

Section: Histologic Analysismentioning

confidence: 99%

TRPV1 Agonist Capsaicin Attenuates Lung Ischemia-Reperfusion Injury in Rabbits

Wang

et al. 2012

Journal of Surgical Research

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“…However, alternative forms of PFC application such as aerosol or vapor administration have shown promising effects without causing typical problems and shortcomings of liquid ventilation. In this context, vaporized PFH was associated with improved oxygenation and lung mechanics in experimental ARDS, protected animals from ventilator-induced lung injury, and improved lung histology in oleic acid-injured pigs to a far greater degree compared with liquid ventilation (3,8,13,45). Whether the anti-inflammatory effects of PFH shown by us in recent in vitro studies contribute to this organoprotection remains unclear (15,25).…”

Section: Discussionmentioning

confidence: 93%

“…These data and the fact that PFC per se do not augment in vitro bacterial growth suggest that nosocomial pneumonia is not increased by liquid ventilation with PFC (29,40). There are no animal trials using PFH in the setting of bacterial lung infection, and the experimental protocols of all studies performed in this field were too short to allow development of VAP (3,8,13,45). However, our in vitro results support the assumption that despite previously demonstrated anti-inflammatory effects (15,25), PFH does not seem to impair lung bactericidal defense, and therefore our initial hypothesis cannot be confirmed.…”

Section: Discussionmentioning

confidence: 99%

Leukocyte antibacterial functions are not impaired by perfluorocarbon exposure in vitro

Haufe¹,

Koenigshausen²,

Knels³

et al. 2008

American Journal of Physiology-Lung Cellular and Molecular Phys

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Application of liquid, aerosolized, and vaporized perfluorocarbons (PFC) in acute lung injury has shown anti-inflammatory effects. Although this may be beneficial in states of pulmonary hyperinflammation, it also could increase susceptibility to nosocomial lung infection. We hypothesized that PFC impair cellular host defense and therefore investigated in an in vitro model the influence of perfluorohexane (PFH) on crucial mechanisms of bacterial elimination in human neutrophils and monocytes. Using scanning and transmission electron microscopy, we could show membrane-bound and ingested PFH particles that morphologically did not alter adherence and phagocytosis of Escherichia coli or leukocyte viability. The amount of adherent and phagocytosed bacteria as determined by flow cytometry was not influenced in cells only pretreated with PFH for 1 and 4 h. When PFH was present during E. coli challenge, bacterial adherence was decreased in polymorphonuclear neutrophils, but respective intracellular uptake was not impaired and was even significantly promoted in monocytes. Overall, E. coli-induced respiratory burst capacity was not reduced by PFH. Our findings provide evidence that key functions of innate host defense are not compromised by PFH treatment in vitro.phagocytosis; burst; perfluorohexane; anti-inflammatory; immune LIQUID VENTILATION WITH PERFLUOROCARBONS (PFC) has been successfully applied in experimental and human acute lung injury (18,20,27,36). Beside positive effects on gas exchange, lung mechanics, and ventilation/perfusion matching, animals treated with liquid ventilation also exhibited attenuated pulmonary damage with concomitant reduction in pulmonary neutrophil sequestration (19,21,32,39). A clinical trial investigating PFC treatment in trauma patients with acute lung injury demonstrated reduced neutrophil counts and decreased levels of proinflammatory cytokines in bronchoalveolar lavage fluid (7). Although removal of alveolar inflammatory exudate by dense, water-insoluble PFC probably contributes to mitigation of pulmonary hyperinflammation, a direct interference with immune cell function may also be involved. This proposition is supported by recent in vivo studies using aerosolized PFC in a surfactant-washout model that reported PFC to decrease mRNA expression of proinflammatory cytokines and adhesion molecules in lung tissue samples as well as in microdissected alveolar macrophages and pulmonary parenchymal cells (42,49,50). Further evidence for cellular anti-inflammatory effects of PFC is provided by in vitro experiments showing a decreased response of activated polymorphonuclear neutrophils (PMN) and monocytic cells in terms of cytokine secretion, chemotaxis, cyclooxygenase-2 (COX-2) expression, and NF-B activation (5,25,37,46,51). Liquid ventilation with PFC did not result in improved survival or reduced need for ventilatory support in recent multicenter trials of patients with acute respiratory distress syndrome (ARDS) (17, 24). Nevertheless, intrinsic anti-inflammatory properties of PFC might b...

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Comparative Effects of Vaporized Perfluorohexane and Partial Liquid Ventilation in Oleic Acid– induced Lung Injury

Cited by 31 publications

References 23 publications

Comparative effects of proportional assist and variable pressure support ventilation on lung function and damage in experimental lung injury*

Comparative effects of proportional assist and variable pressure support ventilation on lung function and damage in experimental lung injury*

TRPV1 Agonist Capsaicin Attenuates Lung Ischemia-Reperfusion Injury in Rabbits

Leukocyte antibacterial functions are not impaired by perfluorocarbon exposure in vitro

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