Comparative antibacterial efficacy of metal oxide nanoparticles against Gram negative bacteria

In vitro assessment of Ag2O nanoparticles toxicity against Gram-positive and Gram-negative bacteria

“…The Ag 2 O nanoparticles used in the present study were characterized through X-ray diffraction (XRD), Fourier transform infra-red spectroscopy (FT-IR) and simultaneous differential thermal analysis (TG-DTG-DTA) and described in an earlier study by Negi et al (2011b).…”

“…It also illustrated that the diameter of the inhibition zone of Ag 2 O nanoparticles increased with the increasing concentrations from 10 to 50 mg per well (Table 2), suggesting the concentration-dependent antibacterial activity of nanoparticles (Negi et al, 2011b;Shamli et al, 2011).…”

“…The experiment was conducted in duplicate under the same set of conditions. The Ag 2 O nanoparticles used in the present study were characterized through X-ray diffraction (XRD), Fourier transform infra-red spectroscopy (FT-IR) and simultaneous differential thermal analysis (TG-DTG-DTA) and described in an earlier study by Negi et al (2011b bacterial activity of Ag 2 O nanoparticles and antibiotics against used test bacteria. One milliliter bacterial midlog phase culture (as mentioned in section Agar diffusion assay) was added to 10 ml lukewarm molten soft agar (0.7%) and poured over solidified nutrient Hiveg agar petriplates.…”

“…However, it is reported that the antibacterial effect of nanoparticles was found to be more pronounced on Gram-negative bacterial strains in virtue of their permeable cell membrane (Sinha et al, 2011). Thus, considering the above facts the present study was planned in continuation to an earlier study (Negi et al, 2011b) to further assess the dose and time-dependent toxicity of antibacterial Ag 2 O nanoparticles against avirulent strains of both Grampositive (Bacillus circulans strain D1 and Bacillus aerius strain SPT2) and Gram-negative bacteria (Escherichia coli strain RGR13 and Pseudomonas aeruginosa strain PS1) through agar diffusion, microdilution and luciferin based ATP assay.…”

“…which have been studied against different Gram-negative and Gram-positive bacteria (Alrousan et al, 2009;Chwalibog et al, 2010;Nangmenyi et al, 2009;Neal, 2008;Negi et al, 2011b;Shameli et al, 2011) as well as multidrug-resistant bacteria (Huh and Kwon, 2011). However, it is reported that the antibacterial effect of nanoparticles was found to be more pronounced on Gram-negative bacterial strains in virtue of their permeable cell membrane (Sinha et al, 2011).…”