Comparative Anatomy of Laboratory Animal Corneas with a New-Generation High-Resolution<i>In Vivo</i>Confocal Microscope

Labbé, Antoine; Liang, Hong; Martin, Chantal; Brignole-Baudouin, Françoise; Warnet, Jean‐Michel; Baudouin, Christophe

doi:10.1080/02713680600701513

Cited by 57 publications

(67 citation statements)

References 43 publications

(18 reference statements)

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“…The HRT II/RCM (Heidelberg Engineering GmbH, Heidelberg, Germany) in vivo confocal microscope for animal studies was used as reported. 37 Briefly, after anesthesia, the rats were positioned sideways under the objective. Several confocal microscopic images of the conjunctival vessels of each eye were taken.…”

Section: In Vivo Confocal Microscopymentioning

confidence: 99%

Effects of ciliary muscle plasmid electrotransfer of TNF-α soluble receptor variants in experimental uveitis

et al. 2009

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Intraocular inflammation has been recognized as a major factor leading to blindness. Because tumor necrosis factor-a (TNF-a) enhances intraocular cytotoxic events, systemic anti-TNF therapies have been introduced in the treatment of severe intraocular inflammation, but frequent re-injections are needed and are associated with severe side effects. We have devised a local intraocular nonviral gene therapy to deliver effective and sustained anti-TNF therapy in inflamed eyes. In this study, we show that transfection of the ciliary muscle by plasmids encoding for three different variants of the p55 TNF-a soluble receptor, using electrotransfer, resulted in sustained intraocular secretion of the encoded proteins, without any detection in the serum. In the eye, even the shorter monomeric variant resulted in efficient neutralization of TNF-a in a rat experimental model of endotoxininduced uveitis, as long as 3 months after transfection. A subsequent downregulation of interleukin (IL)-6 and iNOS and upregulation of IL-10 expression was observed together with a decreased rolling of inflammatory cells in anterior segment vessels and reduced infiltration within the ocular tissues. Our results indicate that using a nonviral gene therapy strategy, the local self-production of monomeric TNF-a soluble receptors induces a local immunomodulation enabling the control of intraocular inflammation.

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Section: In Vivo Confocal Microscopymentioning

confidence: 99%

Effects of ciliary muscle plasmid electrotransfer of TNF-α soluble receptor variants in experimental uveitis

et al. 2009

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“…The use of this microscope has been previously described [19, 20]. Before in vivoconfocal microscopy examination, animals were intramuscularly anaesthetized with xylazine 2 mg/kg body weight and ketamine 50 mg/kg body weight.…”

Section: Methodsmentioning

confidence: 99%

Ocular Burn: Rinsing and Healing with Ionic Marine Solutions and Vegetable Oils

et al. 2008

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Purpose: We investigated the effects of various rinsing and healing protocols on corneal wound repair and inflammation following alkali burn in rabbits. Methods: We conducted in vitro, in vivo and ex vivo studies. First, different rinse solutions were tested in vitro after incubation of ocular cells with methanol or NaOH. Cell viability was then assessed using the neutral red test (cytofluorometry). Second, NaOH was applied to rabbit corneas and associations of rinse solutions (NaCl 0.9% or controlled ionization marine solutions) with N-acetylcysteine or vegetable oils (from Calophyllum inophyllum and Aleurites moluccana) were tested in vivo. The regeneration of the corneal epithelium and the infiltration of inflammatory cells were evaluated using in vivo confocal microscopy and ex vivo histological cuts. Results: The association of a controlled ionization marine solution with 10% C. inophyllum oil and 90% A. moluccana oil induced regeneration of the corneal epithelium and a decrease in inflammatory cells. Conclusions: Irrigation with marine solution followed by treatment with a mixture of C. inophyllum and A. moluccana oils is a promising treatment for ocular burns.

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“…The 3-dimensional organization of the keratocyte network has already been investigated in detail, especially on confocal microscopy. [17][18][19][20][21] The keratocyte cell density is highest in the anterior stroma, and the keratocytes have long processes forming a dense keratocyte network so that the absence of interfibrillar cross-links because of the keratocyte spaces should be significant. The formation of intrafibrillar cross-links as evidenced by the increase in fiber diameter after cross linking 22 should not be affected by this phenomenon, however.…”

Section: Discussionmentioning

confidence: 99%

Significance of the Lacunar Hydration Pattern After Corneal Cross Linking

Wollensak

Herbst

2010

Cornea

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After riboflavin/UV-A cross linking of in vivo rabbit cornea, a characteristic lacunar hydration pattern can be observed in the anterior stroma with maximum cross linking, whereas diffuse edema is present in the adjacent areas without significant cross linking. The lacunar edema may explain the temporary demarcation of the anterior stroma after cross linking on biomicroscopy because of increased light scattering. The network pattern of cross linking may contribute to the elasticity of the cornea after cross linking.

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Comparative Anatomy of Laboratory Animal Corneas with a New-Generation High-ResolutionIn VivoConfocal Microscope

Abstract: The Rostock Cornea Module of the HRT II can provide high-resolution images of all corneal layers of rabbits, rats, and mice without sacrificing animals or preparing tissue. This new device may be useful for evaluating the cornea during experimental animal studies.

Cited by 57 publications

References 43 publications

Effects of ciliary muscle plasmid electrotransfer of TNF-α soluble receptor variants in experimental uveitis

Effects of ciliary muscle plasmid electrotransfer of TNF-α soluble receptor variants in experimental uveitis

Ocular Burn: Rinsing and Healing with Ionic Marine Solutions and Vegetable Oils

Significance of the Lacunar Hydration Pattern After Corneal Cross Linking

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