COMPARATIVE ANALYSIS OF TRANSCRIPTION AND PROTEIN RELEASE OF THE INFLAMMATORY CYTOKINES INTERLEUKIN-1 BETA (IL-1β) AND INTERLEUKIN-8 (IL-8) FOLLOWING MAJOR BURN AND MECHANICAL TRAUMA

Schinkel, Christian; Zimmer, S; Jp, Kremer; Walz, Antje-Christine; Rordorf-Adam, C; G, Henckel von Donnersmarck; Faist, Eugen

doi:10.1097/00024382-199510000-00002

Cited by 21 publications

(10 citation statements)

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“…Significant alterations of proinflammatory IL-6 levels after burn injuries have been shown previously, and authors suggested that early alterations in serum levels of IL-6 constitute a useful predictive marker for identifying patients with concomitant inhalation injury and high mortality. Nevertheless, the role of IL-6 in critically ill and burn-injured patients as a factor contributing to tissue injury has been discussed controversially in the past [11,23,28]. However, in our study values of IL-6 did not significantly differ between patients who died compared with patients who survived, and predictive accuracy did not reach levels of cf-DNA/NETs and PCT.…”

Section: Discussioncontrasting

confidence: 73%

“…The nature of burn injury is a multicomplex pathology, related to injury severity, including burn surface area and depth of injury, source of burn trauma, and amount of inflammatory reaction [22][23][24]. All these aspects combined with individual variability limit the usefulness of existing scores or markers to predict outcome after burn injury.…”

Section: Discussionmentioning

confidence: 99%

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Neutrophil-derived circulating free DNA (cf-DNA/NETs), a potential prognostic marker for mortality in patients with severe burn injury

Altrichter

Zedler

Kraft

et al. 2010

Eur J Trauma Emerg Surg

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The predictive value of circulating free DNA/neutrophil extracellular traps (cf-DNA/NETs) has recently been shown in patients with major trauma for sepsis, multiple organ failure, and mortality. Here we report on the predictive potential of cf-DNA/NETs for mortality in patients with severe burn injury. In a prospective study 32 patients with severe burn injury were included. Blood samples were sequentially obtained on day 1, 3, 5, and 7 after admission. cf-DNA/NETs was directly quantified from plasma by means of rapid fluorescence assay. Time kinetics of cf-DNA/NETs were correlated with clinical data, C-reactive protein (CRP), procalcitonin (PCT), and interleukin (IL)-6. Furthermore sensitivity, specificity, and positive and negative predictive value, as well as receiver operation characteristic (ROC) curves were calculated. Seven patients died within the first month after burn injury. cf-DNA/NETs values from these patients were significantly increased already on day 1 and 3 after admission compared with patients who survived (p < 0.01). In contrast, PCT levels of nonsurvivors were significantly elevated on day 3 and 5 (p < 0.01), while CRP and IL-6 did not show any significant difference between survivors and nonsurvivors. At a cutoff of 255 ng/ml, cf-DNA/NETs had sensitivity of 0.8 and specificity of 0.74. ROC revealed largest areas under the curve (AUC) for cf-DNA/NETs on day 1 (0.851) and 3 (0.883) after admission. For all values between day 1 and 7, AUC was 0.815. cf-DNA/NETs seems to be a rapid, valuable marker for prediction of mortality in burn patients. A larger confirmation trial ought to be carried out.

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Section: Discussioncontrasting

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Neutrophil-derived circulating free DNA (cf-DNA/NETs), a potential prognostic marker for mortality in patients with severe burn injury

Altrichter

Zedler

Kraft

et al. 2010

Eur J Trauma Emerg Surg

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“…Proinflammatory factors, such as tumor necrosis factor-α (TNF-α), C5a and leukotriene B4, chemoattractants (IL-8 and formyl-methionyl-leucyl-phenylalanine) and some growth factors (platelet-derived growth factor) were reported to be potent stimuli that upregulate the expression of CD11b [41][42][43]. Both clinical and experimental studies had demonstrated that numerous proinflammatory factors, such as TNF-α, IL-8 and C5a, could be induced by thermal injuries [25,[44][45][46]. So, the increased expression of CD11b on neutrophils and mono- Granulocytes were identified and gated by forward-and side-light scattering characteristics represented by MLFI using FACS assay.…”

Section: Discussionmentioning

confidence: 99%

Lactosyl derivatives function in a rat model of severe burn shock by acting as antagonists against CD11b of integrin on leukocytes

Zhao

Hu³

et al. 2008

Glycoconj J

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Severe burn shock remains an unsolved clinical problem with urgent needs to explore novel therapeutic approaches. In this study, the in vivo bioactivity of a series of synthetic lactosyl derivatives (oligosaccharides) was assessed on rats with burn shock to elucidate the underlying mechanisms. Administration of An-2 and Gu-4, two lactosyl derivatives with di- and tetravalent beta-D: -galactopyranosyl-(1-4)-beta-D: -glucopyranosyl ligands, significantly prolonged the survival time (P < 0.05 vs. saline), stabilized blood pressure and ameliorated the injuries to vital organs after burn. Flow chamber assay displayed that An-2 and Gu-4 markedly decreased the adhesion of leukocytes to microvessel endothelial cells. Competitive binding assay showed that a CD11b antibody significantly interrupted the interaction of An-2 and Gu-4 with leukocytes from rats with burn shock. With fluorescent microscopy, we further found that the oligosaccharides were selectively bound to leukocytes and with a colocalization of CD11b on the cell membrane. Interestingly, the lectin domain-deficient form of CD11b failed to bind with An-2 and Gu-4. The results suggest that both An-2 and Gu-4 significantly inhibit the adhesion of leukocytes to endothelial cells by binding to CD11b and thereby exert protective effects on severe burn shock.

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“…The effects of EPO have also been observed on other cell types, including myocardial, vascular smooth muscle, and mesangial cells (11,12). The finding of EPO receptors on endothelial cells prompted several in vitro and in vivo investigations aimed to determine whether the hormone may directly affect some of the functions of these cells (13); more specifically, attention has been paid to the complex network of cytokines and growth factors involved in the maturation of red blood cells and the proliferation of endothelial cells and angiogenesis (14)(15)(16)(17). The hypothesis that hematopoietic and endothelial cells share a common hemangioblast progenitor is based on the finding that both cell lineages express cell surface antigens such as CD31 and CD34 (18,19).…”

Section: Introductionmentioning

confidence: 99%

Recombinant Human Erythropoietin Stimulates Angiogenesis and Healing of Ischemic Skin Wounds

Buemi

Galeano

Sturiale³

et al. 2004

Shock

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Wound healing in ischemic tissues such as flap margins due to inadequate blood supply is still a source of considerable morbidity in surgical practice. Adequate tissue perfusion is particularly important in wound healing. We investigated the effects of recombinant human erythropoietin (rHuEPO) on wound healing in an ischemic skin wound model. Sixty-three Sprague-Dawley rats were used. Normal incisional wound and H-shaped double flaps were used as the wound models. Animals were treated with rHuEPO (400 IU/kg) or its vehicle. Rats were killed on different days (3, 5, and 10 days after skin injury) and the wounded skin tissues were used for immunohistochemistry and for analysis of vascular endothelial growth factor content and collagen content. Tissue transglutaminase immunostaining of histological specimens was used as a vascular marker to determine the level of microvessel density. The results showed a higher level of vascular endothelial growth factor protein and an increased microvessel density in ischemic wounds with rHuEPO treatment than the normal incisional wounds and ischemic control wounds. Collagen content was higher in the incisional wounds and in the ischemic wounds with rHuEPO treatment compared with the ischemic control wounds. Our results suggest that erythropoietin may be an effective therapeutic approach in improving healing in ischemic skin wounds.

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COMPARATIVE ANALYSIS OF TRANSCRIPTION AND PROTEIN RELEASE OF THE INFLAMMATORY CYTOKINES INTERLEUKIN-1 BETA (IL-1β) AND INTERLEUKIN-8 (IL-8) FOLLOWING MAJOR BURN AND MECHANICAL TRAUMA

Cited by 21 publications

References 0 publications

Neutrophil-derived circulating free DNA (cf-DNA/NETs), a potential prognostic marker for mortality in patients with severe burn injury

Neutrophil-derived circulating free DNA (cf-DNA/NETs), a potential prognostic marker for mortality in patients with severe burn injury

Lactosyl derivatives function in a rat model of severe burn shock by acting as antagonists against CD11b of integrin on leukocytes

Recombinant Human Erythropoietin Stimulates Angiogenesis and Healing of Ischemic Skin Wounds

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