Comparative analysis of mitochondrial genomes reveals family-specific architectures and molecular features in scorpions (Arthropoda: Arachnida: Scorpiones)

Moreno-Carmona, Manuela; Montaña-Lozano, Paula; Quiroga, Carlos Fernando Prada; Baeza, Juán Antonio

doi:10.1016/j.gene.2023.147189

Cited by 2 publications

(2 citation statements)

References 64 publications

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“…Mitochondrial genomes and full-length nuclear rDNA have been now routinely applied to assess species boundaries and deep relationships in many phylogenetic studies due to obtaining sufficient data efficiently through NGS ( Jia et al, 2023 ; Moreno-Carmona et al, 2023 ; Prada et al, 2023 ). However, a complete mitochondrial genome of any Barbronia species or of the family Salifidae remains unpublished, highlighting a significant gap in our understanding of their systematics.…”

Section: Discussionmentioning

confidence: 99%

Exploring Barbronia species diversity and phylogenetic relationship within Suborder Erpobdelliformes (Clitellata: Annelida)

Liu,

Fu,

Wang

et al. 2024

PeerJ

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Background Barbronia, a genus of freshwater macrophagous leeches, belongs to Erpobdelliformes (Salifidae: Clitellata: Annelida), and B. weberi, a well-known leech within this genus, has a worldwide distribution. However, the systematics of Barbronia have not yet been adequately investigated, primarily due to a few molecular markers, and only 20 Barbronia sequences available in the GenBank database. This gap significantly limits our understanding of the Barbronia species identification, as well as the phylogenetic placement of the genus Barbronia within Salifidae. Methods Next-generation sequencing (NGS) was used to simultaneously capture the entire mitochondrial genome and the full-length 18S/28S rDNA sequences. The species boundary of Barbronia species was estimated using bGMYC and bPTP methods, based on all available Barbronia COI sequences. Uncorrected COI p-distance was calculated in MEGA. A molecular data matrix consisting of four loci (COI, 12S, 18S, and 28S rDNA) for outgroups (three Haemopis leeches) and 49 erpobdellid leeches, representing eight genera within the Suborder Erpobdelliformes was aligned using MAFFT and LocARNA. This matrix was used to reconstruct the phylogenetic relationship of Barbronia via Bayesian inference (BI) and the maximum likelihood (ML) method. Results The full lengths of the mitochondrial genome, 18S and 28S rDNAs of B. cf. gwalagwalensis, are 14847 bp, 1876 bp 1876 bp, and 2863 bp, respectively. Both bGMYC and bPTP results based on COI data are generally congruent, suggesting that the previously proposed taxa (B. arcana, B. weberi formosana, and B. wuttkei or Erpobdella wuttkei) are synonyms of B. weberi. The specimens listed in the B. gwalagwalensis group, however, are split into at least two Primary Species Hypotheses (PSHs). The p-distance of the first PSH is less than 1.3% but increased to 4.5% when including the secondary PSH (i.e., B. cf. gwalagwalensis). In comparison, the interspecific p-distance between the B. weberi group and the B. gwalagwalensis group ranged from 6.4% to 8.7%, and the intraspecific p-distance within the B. weberi group is less than 0.8%. Considering the species delimitation results and the sufficient large p-distance, the specimen sampled in China is treated as B. cf. gwalagwalensis. The monophyly of the four Erpobdelliformes families Salifidae, Orobdellidae, Gastrostomobdellidae sensu stricto and Erpobdellidae is well supported in ML and BI analysis based on a data of four markers. Within the Salifidae, a well-supported Barbronia is closely related to a clade containing Odontobdella and Mimobdella, and these three genera are sister to a clade consisted of Salifa and Linta. According to the results of this study, the strategy of simultaneous obtaining both whole mitochondria and nuclear markers from extensively sampled Salifids species using NGS is expected to fathom both the species diversity of B. gwalagwalensis and the evolutionary relationship of Salifidae.

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Section: Discussionmentioning

confidence: 99%

Exploring Barbronia species diversity and phylogenetic relationship within Suborder Erpobdelliformes (Clitellata: Annelida)

Liu,

Fu,

Wang

et al. 2024

PeerJ

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“…Arthropod is one of the diverse group on earth [ 1 , 2 ] and Arachnida is one of the important class belonging to it. With more than 2,700 species, scorpions are a category of arachnids that have significant evolutionary success within invertebrates [ 3 ]. With about 138 species in 10 genera, Diplocentridae Karsch, 1880 is considered native to the New World and Middle East [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

The phylogenetic relationship among two species of genus Nebo (Scorpiones: Diplocentridae) from Saudi Arabia and Middle East

Alqahtani¹,

Alotaibi²,

Aly³

et al. 2023

BMC Zool

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Background The genus Nebo has been identified as a medically important scorpion species distributed across Arabia and the Middle East. However, its taxonomic status remains unclear. Aim The molecular phylogeny of two Nebo species from Saudi Arabia and comparative sequences from Palestine is presented based on the mitochondrial cytochrome oxidase subunit I (COI) gene. Methodology Scorpion specimens were collected from two different localities, mainly the Southern part of Saudi Arabia. Then, DNA was extracted, amplified using invertebrate universal primers, and sequenced to identify the COI gene. The obtained sequences were analyzed, and phylogenetic trees based on maximum parsimony, neighbor-joining, and Bayesian inference were constructed. Results The inferred phylogeny indicates the monophyletic status of the family Diplocentridae and its subfamily Nebinae and Diplocentrinae. Also, the phylogenetic analyses support the existence of interspecific and intraspecific variations among/ within Nebo hierichonticus and Nebo yemenensis which may indicate distinct species. Conclusion Further morphological studies with additional specimens from the Arabian Peninsula may reveal possible undiscovered and cryptic species.

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Comparative analysis of mitochondrial genomes reveals family-specific architectures and molecular features in scorpions (Arthropoda: Arachnida: Scorpiones)

Cited by 2 publications

References 64 publications

Exploring Barbronia species diversity and phylogenetic relationship within Suborder Erpobdelliformes (Clitellata: Annelida)

Exploring Barbronia species diversity and phylogenetic relationship within Suborder Erpobdelliformes (Clitellata: Annelida)

The phylogenetic relationship among two species of genus Nebo (Scorpiones: Diplocentridae) from Saudi Arabia and Middle East

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