Comparative analysis of enzymatic activity of tannase in non-conventional yeasts to produce ellagic acid

Márquez-López, Anahí; Ramírez-Conejo, Juan David; Chávez-Parga, Ma. del Carmen; Flores, Dora Cecilia Valencia; Jaramillo, Miguel Ángel Zamudio; Rodríguez, Horacio González; González‐Hernández, Juan Carlos

doi:10.1590/fst.12519

Cited by 3 publications

(1 citation statement)

References 14 publications

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“…However, they had pH values from 0.5 to 0.8 units, far from the optimum. They decreased abruptly in treatment seven at hour 24, having null tannase activity; in general, it presented an inconsistent activity during all treatments, reaching null values in some points such as It has already been mentioned, which has two possible explanations, the first is that it is because this enzyme is inducible (Doi et al, 1973), requiring the presence of tannic acid in the medium so that it could be expressed, which was observed in the experiments carried out by Márquez-López et al (2020), showing that by increasing the concentration of inducer the enzymatic activity also increases.…”

Section: Activities Enzymatic Tannase and β-Glucosidasementioning

confidence: 85%

Optimization of the ellagic acid synthesis process at the bioreactor level using non-conventional yeasts

Madrigal-Chávez,

Ruíz-Pompa,

Márquez-López

et al. 2023

Braz. J. Pharm. Sci.

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Ellagic acid (EA) is a phenolic biomolecule. For its biosynthesis, a source of ellagitannins is required, such as strawberries and yeasts, as precursors of the tannase and β-glucosidase enzymes responsible for hydrolysis of ellagitannins. Two experimental mixture designs were applied., varying the yeast concentration and the number of ellagitannins in the culture medium, evaluating the enzymatic activity and ellagic acid biosynthesis. Aiming to find the optimal compositions of the non-conventional yeasts assessed in the research to biosynthesize ellagic acid feasibly and efficiently using a response surface performing the statistical analysis in the StatGraphics® program for obtaining a higher yield and optimizing the ellagic acid synthesis process, the results indicate that the strains Candida parapsilosis ITM LB33 and Debaryomyces hansenii ISA 1510 have a positive effect on the synthesis of ellagic acid, since as its concentration increases in the mixture the concentration of ellagic acid in the medium also increases; on the other hand, the addition of Candida utilis ITM LB02 causes a negative effect, resulting in the compositions of 0.516876, 0.483124 and 2.58687E -9 respectively, for a treatment under the same conditions, an optimal value of ellagic acid production would be obtained. With an approximate value of 7.33036 mg/mL.

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Section: Activities Enzymatic Tannase and β-Glucosidasementioning

confidence: 85%

Optimization of the ellagic acid synthesis process at the bioreactor level using non-conventional yeasts

Madrigal-Chávez,

Ruíz-Pompa,

Márquez-López

et al. 2023

Braz. J. Pharm. Sci.

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Comparative analysis of β-glucosidase activity in non-conventional yeasts

GONZÁLEZ-HERNÁNDEZ,

RAMÍREZ-CONEJO,

GARCÍA-AGUIRRE

2023

An. Acad. Bras. Ciênc.

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Production, Purification and Characterization of Extracellular Tannase from a Newly Isolated Yeast, Geotrichum cucujoidarum

Thangavelu¹,

Hugar²,

Belur³

2022

J. Pure Appl. Microbiol.

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With an aim to isolate a tannase positive organism, the microbial mat growing on the stored areca extract leachate surface was screened. Once the tannase positive organism was isolated, it was identified by ITS/18S rRNA gene sequencing. Further, the enzyme was purified and examined for its biochemical properties. A potent extracellular tannase-producing yeast was isolated and was identified as Geotrichum cucujoidarum. After the shake flask studies, the enzyme activity of 4.42 U/ml and specific activity of 29.86 U/mg were achieved in a medium with tannic acid as an inducer. Later, ethanol (70%) precipitation followed by purification through FPLC using SEC 650 column resulted in 166.37 U/mg specific activity and a recovery of 50.54%. The purified enzyme was a monomer with a molecular weight of 63 kDa. The optimum pH and the temperature of the enzyme were found to be 5.0 and 30°C, respectively. The Michaelis-Menten constant (Km) was found to be 2.9 mM, and the turn over number (kcat) and catalytic efficiency (kcat/km) of the purified tannase were 102 S-1 and 35.17 mM-1S-1 respectively. Temperature and pH stability profiles of the enzyme, influence of various metal ions, chelators and surfactants on enzyme activity and kinetic constants of enzyme shows that the tannase produced from Geotrichum cucujoidarum is unique and is a potential candidate for further studies.

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Comparative analysis of enzymatic activity of tannase in non-conventional yeasts to produce ellagic acid

Cited by 3 publications

References 14 publications

Optimization of the ellagic acid synthesis process at the bioreactor level using non-conventional yeasts

Optimization of the ellagic acid synthesis process at the bioreactor level using non-conventional yeasts

Comparative analysis of β-glucosidase activity in non-conventional yeasts

Production, Purification and Characterization of Extracellular Tannase from a Newly Isolated Yeast, Geotrichum cucujoidarum

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