Comparative analyses on medium optimization using<i>one-factor-at-a-time</i>, response surface methodology, and artificial neural network for lysine–methionine biosynthesis by<i>Pediococcus pentosaceus</i>RF-1

Nor, Norfarina Muhamad; Mohamed, Mona H.; Loh, Teck Chwen; Foo, Hooi Ling; Rahim, Raha Abdul; Tan, Joo Shun; Mohamad, Rosfarizan

doi:10.1080/13102818.2017.1335177

Cited by 54 publications

(38 citation statements)

References 24 publications

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“…Lactic acid bacteria (LAB) appear as an exceptional candidate owing to their non-pathogenic nature and reputation as Generally Recognized as Safe (GRAS) microorganism [9]. Moreover, numerous studies have reported that LAB possess capability to produce various amino acids [10–18]. Nevertheless, there were limited documentation on the application of LAB for amino acid production.…”

Section: Introductionmentioning

confidence: 99%

Optimized medium via statistical approach enhanced threonine production by Pediococcus pentosaceus TL-3 isolated from Malaysian food

et al. 2019

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Background: Threonine is an essential amino acid that is extensively used in livestock industry as feed supplement due to its pronounced effect in improving the growth performance of animals. Application of genetically engineered bacteria for amino acid production has its share of controversies after eosinophils myalgia syndrome outbreak in 1980s. This has urged for continuous search for a food grade producer as a safer alternative for industrial amino acid production. Lactic acid bacteria (LAB) appear as an exceptional candidate owing to their non-pathogenic nature and reputation of Generally Recognized as Safe (GRAS) status. Recently, we have identified a LAB, Pediococcus pentosaceus TL-3, isolated from Malaysian food as a potential threonine producer. Thus, the objective of this study was to enhance the threonine production by P. pentosaceus TL-3 via optimized medium developed by using Plackett-Burman design (PBD) and central composite design (CCD). Results: Molasses, meat extract, (NH 4) 2 SO 4 , and MnSO 4 were identified as the main medium components for threonine production by P. pentosaceus TL-3. The optimum concentration of molasses, meat extract, (NH 4) 2 SO 4 and MnSO 4 were found to be 30.79 g/L, 25.30 g/L, 8.59 g/L, and 0.098 g/L respectively based on model obtained in CCD with a predicted net threonine production of 123.07 mg/L. The net threonine production by P. pentosaceus TL-3 in the optimized medium was enhanced approximately 2 folds compared to the control. Conclusions: This study has revealed the potential of P. pentosaceus TL-3 as a safer alternative to produce threonine. Additionally, the current study has identified the key medium components affecting the production of threonine by P. pentosaceus TL-3, followed by optimization of their concentrations by means of statistical approach. The findings of this study could act as a guideline for the future exploration of amino acid production by LAB.

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Section: Introductionmentioning

confidence: 99%

Optimized medium via statistical approach enhanced threonine production by Pediococcus pentosaceus TL-3 isolated from Malaysian food

et al. 2019

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“…The OFAT methodology allows studying one variable at a time, and implementing its optimal conditions to the analyses of the subsequent variables [14]. The OFAT methodology to study the growth parameters of L. crescens is evaluated using four criteria: pH optimization, aeration, temperature preference, and buffer requirements (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Here, the optimal growth conditions of L. crescens are presented for four growth parameters—pH, aeration, temperature and buffering capacity—using one factor at a time methodology (OFAT) [14]. The evaluation of the growth parameters of L. crescens gave insight into the physiology, lifestyle and culturability of the Ca.…”

Section: Introductionmentioning

confidence: 99%

Growth parameters of Liberibacter crescens suggest ammonium and phosphate as essential molecules in the Liberibacter-plant host interface

et al. 2019

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Background Liberibacter crescens is the closest cultured relative of four important uncultured crop pathogens. Candidatus. L. asiaticus, L. americanus, L. africanus cause citrus greening disease, while Ca. L. solanacearum causes potato Zebra chip disease. None of the pathogens grows in axenic culture. L. crescens grows in three media: a BM-7, a serum-free Hi® Grace’s Insect Medium (Hi-GI), and a chemically-defined medium called M15. To date, no optimal growth parameters of the model species L. crescens have been reported. Studying the main growth parameters of L. crescens in axenic culture will give us insights into the lifestyle of the Ca. Liberibacter pathogens. Results The evaluation of the growth parameters—pH, aeration, temperature, and buffering capacity—reflects the optimal living conditions of L. crescens. These variables revealed that L. crescens is an aerobic, neutrophilic bacterium, that grows optimally in broth in a pH range of 5.8 to 6.8, in a fully oxygenated environment (250 rpm), at 28 °C, and with monosodium phosphate (10 mM or 11.69 mM) as the preferred buffer for growth. The increase of pH in the external media likely results from the deamination activity within the cell, with the concomitant over-production of ammonium in the external medium. Conclusion L. crescens and the Ca. Liberibacter pathogens are metabolically similar and grow in similar environments—the phloem and the gut of their insect vectors. The evaluation of the growth parameters of L. crescens reveals the lifestyle of Liberibacter, elucidating ammonium and phosphate as essential molecules for colonization within the hosts. Ammonium is the main driver of pH modulation by active deamination of amino acids in the L. crescens amino acid rich media. In plants, excess ammonium induces ionic imbalances, oxidative stress, and pH disturbances across cell membranes, causing stunted root and shoot growth and chlorosis—the common symptoms of HLB-disease. Phosphate, which is also present in Ca. L. asiaticus hosts, is the preferred buffer for the growth of L. crescens. The interplay between ammonium, sucrose, potassium (K+), phosphate, nitrate (NO3−), light and other photosynthates might lead to develop better strategies for disease management.

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“…This approach requires less number of experiments, equipments, and financials. In addition, the data obtained from this approach is much more reliable, understandable, can be used for prediction and simulation, and allow for evaluation of the interactions among variables (Singh et al 2011;Nor et al 2017).…”

Section: Optimization Of the Factors Affecting Fungal Chitin And Chitmentioning

confidence: 99%

Current trends in fungal biosynthesis of chitin and chitosan

2019

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Background: Chitin and chitosan are natural biopolymers found in shell of crustaceans, exoskeletons of insects and mollusks, as well as in the cell walls of fungi. These biopolymers have versatile applications in various fields such as biomedical, food industry, and agriculture. These applications are back to their biocompatibility, biodegradability, strong antibacterial effect, and non-toxicity. Outcomes: The fungal biopolymers have many features that made them more advantageous than those biopolymers from seafood waste origin. Chitin and chitosan are not components of cell wall in all fungal species. The fungal classes of Basidiomycetes, Ascomycetes, Zygomycetes, and Deuteromycetes are known to contain chitin and chitosan in their cell walls. The amounts and characters of fungal biopolymers are affected by many factors so they should be optimized before increasing the scale of production. The statistical design of experiments is the most recent and advanced approach for optimization of various factors with more reliable results. Conclusion: Although extensively studied, further studies concerning fungal chitin and chitosan should be conducted in order to be sure of safety for human use.

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Comparative analyses on medium optimization usingone-factor-at-a-time, response surface methodology, and artificial neural network for lysine–methionine biosynthesis byPediococcus pentosaceusRF-1

Cited by 54 publications

References 24 publications

Optimized medium via statistical approach enhanced threonine production by Pediococcus pentosaceus TL-3 isolated from Malaysian food

Optimized medium via statistical approach enhanced threonine production by Pediococcus pentosaceus TL-3 isolated from Malaysian food

Growth parameters of Liberibacter crescens suggest ammonium and phosphate as essential molecules in the Liberibacter-plant host interface

Current trends in fungal biosynthesis of chitin and chitosan

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