Comparative analyses of hypothalamus transcriptomes reveal fertility-, growth-, and immune-related genes and signal pathways in different ploidy cyprinid fish

Li, Shengnan; Zhou, Yi; Yang, Chungang; Fan, Siyu; Huang, Lu; Zhou, Tian; Wang, Qiubei; Zhao, Rurong; Tang, Chenchen; Tao, Min; Liu, Shaojun

doi:10.1016/j.ygeno.2021.01.004

Cited by 13 publications

(4 citation statements)

References 87 publications

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“…Polyploidy has been generated in many fish species (Cherfas et al, 1994;Bramick et al, 1995;Hamasak et al, 2013;Li et al, 2021). Compared with diploid (2n) fish, triploid (3n) fish often have superior phenotypic characteristics, such as an enhanced growth rate, lower mortality rate, and greater stress tolerance (Leggatt and Iwama, 2003;Seehausen, 2004;Nell and Perkins, 2005;Liu et al, 2016;Tao et al, 2018;Wang et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Enhanced Immune Response Improves Resistance to Cadmium Stress in Triploid Crucian Carp

et al. 2021

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Previous research has indicated that triploid crucian carp (3n fish) have preferential resistance to cadmium (Cd) compared to Carassius auratas red var. (2n fish). In this article, comparative research is further conducted between the 2n and 3n fish in terms of the immune response to Cd-induced stress. Exposure to 9 mg/L Cd for 96 h changed the hepatic function indexes remarkably in the 2n fish, but not in the 3n fish. In the serum of Cd-treated 2n fish, the levels of alanine amino transferase, aspartate aminotransferase, adenosine deaminase, and total bilirubin significantly increased, while the levels of total protein, albumin, lysozyme, and anti-superoxide anion radicals decreased demonstrating hepatotoxicity. By analysis of transcriptome profiles, many immune-related pathways were found to be involved in the response of 3n fish to the Cd-induced stress. Expression levels of the immune genes, including the interleukin genes, tumor necrosis factor super family member genes, chemokine gene, toll-like receptor gene, and inflammatory marker cyclooxygenase 2 gene were significantly enhanced in the hepatopancreas of the Cd-treated 3n fish. In contrast, the expression levels of these genes decreased in the 2n fish. This research provides a theoretical basis for polyploid fish breeding and is helpful for the ecological restoration of water due to pollution.

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Section: Introductionmentioning

confidence: 99%

Enhanced Immune Response Improves Resistance to Cadmium Stress in Triploid Crucian Carp

et al. 2021

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“…Heterosis has been widely exploited in aquaculture breeding [3][4][5][6]. Hybrid tilapia, the offspring of maternal O. niloticus and paternal O. aureus, has been demonstrated to have enhanced performance traits, such as faster growth rate, and better stress and disease tolerance [14,15].…”

Section: Discussionmentioning

confidence: 99%

“…This phenomenon has been widely exploited by aquaculture breeders for decades [2], such as in crucian carp (Carassius auratus red var.) [3], Pacific oyster (Crassostrea gigas) [4,5], and scallop (Patinopecten yessoensis ♀× P. caurinus ♂) [6]. Many hypotheses on the genetic mechanism of heterosis have been proposed, including the dominance hypothesis, over-dominance hypothesis, and epistasis hypothesis [7].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Profiling Revealed Basis for Growth Heterosis in Hybrid Tilapia (Oreochromis niloticus ♀ × O. aureus ♂)

Zhu

Zou

et al. 2022

Fishes

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Hybrid tilapia were produced from hybridization of Nile tilapia (Oreochromis niloticus) and blue tilapia (O. aureus). Comparative transcriptome analysis was carried out on the liver of hybrid tilapia and their parents by RNA sequencing. A total of 2319 differentially expressed genes (DEGs) were identified. Trend co-expression analysis showed that non-additive gene expression accounted for 67.1% of all DEGs. Gene Ontology and KEGG enrichment analyses classified the respective DEGs. Gene functional enrichment analysis indicated that most up-regulated genes, such as FASN, ACSL1, ACSL3, ACSL6, ACACA, ELOVL6, G6PD, ENO1, GATM, and ME3, were involved in metabolism, including fatty acid biosynthesis, unsaturated fatty acid biosynthesis, glycolysis, pentose phosphate pathway, amino acid metabolism, pyruvate metabolism, and the tricarboxylic acid cycle. The expression levels of a gene related to ribosomal biosynthesis in eukaryotes, GSH-Px, and those associated with heat shock proteins (HSPs), such as HSPA5 and HSP70, were significantly down-regulated compared with the parent tilapia lineages. The results revealed that the metabolic pathway in hybrid tilapia was up-regulated, with significantly improved fatty acid metabolism and carbon metabolism, whereas ribosome biosynthesis in eukaryotes and basal defense response were significantly down-regulated. These findings provide new insights into our understanding of growth heterosis in hybrid tilapia.

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“…Examples of hybrids with hybrid sterility include the horse and donkey hybrid [19], allotriploid crucian carp [20], and sterile male hybrids of Cobitis taenia and C. elongatoides [21]. In addition, studies of triploid hybrid sh have demonstrated that irregular meiotic prophase I and midphase I arrest [22], aberrant expression of genes involved in cell cycles [22], reproduction [23][24][25][26][27][28][29][30], apoptosis [24,31], and abnormal hormone levels [25] may contribute to their sterility.…”

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confidence: 99%

Reproductive characteristics of reciprocal hybrid lineages derived from hybridization between Megalobrama amblycephala × Culter alburnus

Ding

Zhang

et al. 2023

Preprint

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Background Distant hybridization is an important breeding technique for creating new strains with superior traits by integrating two different genomes. Successful hybridization of Megalobrama amblycephala (BSB, 2n = 48) and Culter alburnus (TC, 2n = 48) was achieved to establish hybrid lineages (BT and TB), which provide valuable materials for exploring the mechanisms of distant hybridization fertility. In this study, the gonadal tissue transcriptomes of BSB, TC, BT-F1, and TB-F1 were sequenced using Illumina high-throughput sequencing technology to analyze the reproductive characteristics of BT and TB.Results Differential gene expression analysis showed that the differentially expressed genes in BT vs BSB and BT vs TC were mainly enriched in signaling pathways not directly associated with meiosis. The differentially expressed genes of TB vs BSB and TB vs TC were mainly enriched in pathways related to meiosis, and most of them were down-regulated, indicating that meiosis is suppressed in TB. UD (under-dominance) genes were enriched in pathways related to meiosis and DNA repair in TB. OD (over-dominance) genes were enriched in MAPK signaling pathway, and ELD-B (expression level dominance-BSB) genes were enriched in pathways related to steroid hormone synthesis in both BT and TB.Conclusions These results suggest that meiotic progression may not be affected in BT, whereas it is clearly inhibited in TB. Offspring of M. amblycephala maternal parent may have better genomic compatibility and fertility. Our study provides important information on the molecular mechanisms of breaking reproductive isolation in distantly hybridized fertile lineages.

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Comparative analyses of hypothalamus transcriptomes reveal fertility-, growth-, and immune-related genes and signal pathways in different ploidy cyprinid fish

Cited by 13 publications

References 87 publications

Enhanced Immune Response Improves Resistance to Cadmium Stress in Triploid Crucian Carp

Enhanced Immune Response Improves Resistance to Cadmium Stress in Triploid Crucian Carp

Transcriptome Profiling Revealed Basis for Growth Heterosis in Hybrid Tilapia (Oreochromis niloticus ♀ × O. aureus ♂)

Reproductive characteristics of reciprocal hybrid lineages derived from hybridization between Megalobrama amblycephala × Culter alburnus

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