Comparal ve susceptibility of three species of char and of rairbow trout x char triploid hybrids to several pathogenic salmonid viruses

Abstract

T n p l o~d hybnds were obtained from rainbow trout Oncorhynchus mykiss females crossed w t h sires of brook trout Salvehnus fontinalis arctic char S alpinus and lake trout S namaycush following heat-shock-provoked retention of the second polar body Survlval rates of h y b r~d s obtained were ca 50 % The hybnds and the parental specles were challenged with infect~ous pancreatic necrosls vlrus (IPNV) type Sp viral haemorrhagic septlcaem~a vlrus (VHSV) types 1 and 3 and lnfect~ous h a e m a t o p o~e t~c necrosis vlrus (IHNV) Ra~nbow trout X coho salmon ( 0 h s u t c h ) t n p l o~d hybnds were included in certain infection t i~a l s The results Indicated that brook trout were reslstant to VHSV and brook trout X ralnbow trout hybnds were reslstant to both VHSV and IHNV but suscept~ble to IPNV Arct~c char were susceptible to IPNV and resistant to VHSV The hybnds were suscept~ble to IPNV, but part~ally reslstant to VHSV Lake trout were partially resistant to IPNV but their hybnds w t h ralnbow trout were susceptible Lake trout infected by VHSV showed c l~n~c a l slgns and sign~ficant losses resulted

Transcriptional differences between triploid and diploid Chinook salmon (Oncorhynchus tshawytscha) during live Vibrio anguillarum challenge

“…However, studies on the immunocompetence of triploid salmonids have proven equivocal (reviewed by Bruno and Johnstone, 1990;Yamamoto and Iida, 1995;Benfey, 1999), but the inconsistent results may be because of individual and familial variation or genotype-by-environmental interactions, not ploidy differences (Johnson et al, 2004(Johnson et al, , 2007Miller et al, 2004). Immune challenge studies with a variety of pathogens resulted in either equal competence of triploid and diploid fish, or triploids faring poorly relative to diploids (Bruno and Johnstone, 1990;Dorson et al, 1991;Ojolick et al, 1995;Johnson et al, 2004). Although the use of triploid salmon in commercial aquaculture has been proposed, it has not been widely adopted, largely because of contradictory findings about the impact of triploidy on performance and survival.…”

In vitro viral haemorrhagic septicaemia virus replication in excised fins of rainbow trout: correlation with resistance to waterborne challenge and genetic variation

“…The VHSV challenge was performed when the fish were 3 to 5 mo old (weight range for all experiments: 1 to 5 g) according to Dorson et al (1991). Briefly, each progeny was duplicated (from 25 to 100 per group) and 1 group was mock-infected and kept for further determination of VREFT.…”

“…VHSV strain 07-71 (serotype 1) isolated from diseased fish from a French trout farm was propagated in epithelioma papulosum cyprini (EPC) cells as described by Dorson et al (1991). After infection, the cells were incubated at 14°C in Stoker's medium (Stoker & MacPherson 1961), buffered at pH 7.4 with 0.16 M Tris-HCl, and supplemented with 2% foetal calf serum and antibiotics (penicillin 100 IU ml -1 , streptomycin 0.1 mg ml -1 and kanamycin 0.1 mg ml -1…”

“…Resale or republication not permitted without written consent of the publisher VHSV serotypes and can transmit their resistance to interspecific hybrids (Dorson & Chevassus 1985, Dorson et al 1991. The different facets of successive infection steps and of resistance mechanisms for the 2 important rhabdovirus diseases of salmonids (VHS and infectious haematopoietic necrosis [IHN], the other important salmonid rhabdoviral disease) have been the object of increasing interest during the past few decades.…”

Revue bibliographique - Principales caractéristiques de l'élevage de l'omble chevalier (Salvelinus alpinus L.) en eau douce

“…Il semble que parmi les principaux virus qui affectent les salmonidés (Septicémie Hémorragique Virale, Nécrose Hématopoïétique Infectieuse, Nécrose Pancréatique Infectieuse) seul le dernier touche l'omble (DORSON et al, 1991 ). Il faut noter que la furonculose, due à la bactérie Aeromonas salmonicida, existe sous une forme chronique chez la plupart des individus.…”