2009
DOI: 10.4322/rbpv.018e1011 View full text |Buy / Rent full text
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Abstract: ResumoOs sinais clínicos das infecções por Ehrlichia canis e Anaplasma platys são similares, e o diagnóstico desses patógenos feito por esfregaços sanguíneos corados é difícil devido à sensibilidade e especificidade. Por outro lado, os diagnósticos moleculares são altamente sensíveis e específicos, e nested-PCRs têm sido otimizadas para o diagnóstico preciso desses patógenos em cães. Em um Hospital Veterinário Escola, amostras de sangue total com EDTA foram obtidas de 100 cães, e esfregaços foram feitos das am… Show more

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“…The occurrence (5.47%) of coinfections in dogs caused by A. platys and E. canis reinforces the hypothesis that these infections are probably transmitted by the same vector; similar results were observed in different geographical regions in Brazil (DANTAS-TORRES, 2008;DAGNONE et al, 2009;RAMOS et al, 2009;SANTOS et al, 2009). This phenomenon was also described in dogs from Thailand (SUKSAWAT et al, 2001b), Venezuela (SUKSAWAT et al, 2001a;HUANG et al, 2005), and Grenada (YABSLEY et al, 2008).…”
Section: Resultssupporting
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“…The occurrence (5.47%) of coinfections in dogs caused by A. platys and E. canis reinforces the hypothesis that these infections are probably transmitted by the same vector; similar results were observed in different geographical regions in Brazil (DANTAS-TORRES, 2008;DAGNONE et al, 2009;RAMOS et al, 2009;SANTOS et al, 2009). This phenomenon was also described in dogs from Thailand (SUKSAWAT et al, 2001b), Venezuela (SUKSAWAT et al, 2001a;HUANG et al, 2005), and Grenada (YABSLEY et al, 2008).…”
Section: Resultssupporting
“…When the result (19.4%) of A. platys was compared with similar studies, it was possible to observe that the prevalence level derived from this study was lower than that described in Campo Grande, State of Mato Grosso do Sul, 42% (DAGNONE et al, 2009), and Recife, State of Pernanbuco, 55% (RAMOS et al, 2009). However, these results were higher than those observed in Rio de Janeiro, State of Rio de Janeiro, 15.84% (FERREIRA et al, 2007), Jaboticabal, State of Sao Paulo, 8% (DAGNONE et al, 2009), and Ribeirao Preto, State of Sao Paulo, 14.9% (SANTOS et al, 2009).…”
Section: Resultsmentioning
“…Aguirre et al (2008) relatam após comparar cinco protocolos diferentes de nPCR com uma PCR convencional, que a nPCR chega a ser 33% mais eϐiciente para a detecção de E. canis. Ramos et al (2009) também recomenda o nPCR para pesquisa de E. canis e A. platys pela maior sensibilidade e especiϐicidade para detecção de ambos os patógenos. Entretanto, uma análise comparativa entre a PCR (gene DSB) e a nested-PCR (16S rRNA), realizada em 24 amostras sangüíneas de cães naturalmente infectados por E. canis, demonstrou que as duas técnicas são adequadas ao diagnóstico da erliquiose monocítica canina (Machado 2004).…”
Section: Methodsunclassified
“…D' Agnone (2006) observou a presença de E. canis em plaquetas de dois cães por meio da nPCR. Sousa (2006) também observou mórulas de E. canis em plaquetas de dois cães e de E. canis e A. platys em outros dois cães de um total de 15 animais pesquisados por nPCR e Ramos et al (2009) através da nPCR observaram que de quatro cães com mórulas em plaquetas, três eram E. canis e apenas uma amostra era A. platys. Neste estudo nenhuma das amostras positivas no esfregaço foram positivas para nenhuma espécie do gênero Ehrlichia sp, sendo necessário maiores investigações acerca da presença das inclusões nas plaquetas.…”
Section: Methodsunclassified
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