Comparability of genotyping data obtained by different procedures – an inter-laboratory survey

Resolving the Parentage of the Apple Cultivar ‘Meran’

“…Dna was isolated from leaf discs using the "nucleoSpin Plant Mini Kit" (Macherey-nagel, Düren, germany). each Dna sample was genotyped at 14 microsatellite loci (cH01c06, cH01d08, cH01f02, cH01f07a, cH02b10, cH02c02a, cH02c09, cH02c11, cH02d08, cH02d12, cH02h11a, cH03a04, cOl, cH01h01;liebhard et al 2002) and analysed at a ceQ 8000 genetic analysis System (Beckman coulter, Fullerton, ca, USa) (Baric et al 2008). allele sizes were assigned using the Fragment analysis Software Version 8.0 from the same manufacturer.…”

Molecular Tools Applied to the Advancement of Fruit Growing in South Tyrol: a Review

“…each group analysed the same set of 61 Dna samples at five microsatellite loci by applying their own laboratory protocols. comparative analysis of the two datasets revealed that 99.3 % of the single-locus genotypes corresponded with at least one allele, but also evidenced the occurrence of inconsistencies due to dropout of longer alleles, mis-scoring of stutter peaks or complete allele mismatch, that could impede the applicability of merged databases (Baric et al 2008b). it was thus concluded, that an interchange of genotyping data needs to be preceded by an a priori standardisation of analysis protocols across different laboratories.…”

“…garkava-gustavsson et al 2008;guarino et al 2006;Hokanson et al 1998;Pereiralorenzo et al 2007). a collaborative study involving the Molecular Biology laboratory and the institute of Plant Sciences of the University of graz addressed the potential for the exchange of genotyping data produced in different laboratories, since the integration of different datasets would be useful for direct comparisons of the genetic diversity of germplasm resources within and among regions or for identification of synonym or homonym cultivars (Baric et al 2008b). each group analysed the same set of 61 Dna samples at five microsatellite loci by applying their own laboratory protocols.…”

Comparative Molecular Genetic Analysis of Apple Genotypes Maintained in Germplasm Collections

“…For this reason, there have been efforts in different apple growing regions to collect the remaining local genetic diversity of this fruit crop (e.g. Baric et al 2008;guarino et al 2006;guilford et al 1997;Pereira-lorenzo et al 2007) and to preserve this diversity in germplasm collections. the role of collections is, however, not only to maintain the agricultural biodiversity as cultural heritage for the future, but also to accurately describe and characterise the accessions in order to use them as potential donors of desirable traits (such as disease resistance or fruit quality) in breeding programmes (Hokanson et al 2001;Kumar et al 2012).…”

“…One of the molecular tools currently widely employed are microsatellite Dna markers or SSrs (Short Sequence repeats), which are highly robust and reproducible (Baric et al 2008(Baric et al , 2011guarino et al 2006;guilford et al 1997;Hokanson et al 1998;Pereira-lorenzo et al 2007;Wünsch and Hormaza 2002) and are not influenced by environmental factors. apart from the determination of genetic identity of single accessions, microsatellites are suitable for the assessment of genetic relationships or estimates of genetic diversity within a germplasm collection (Hokanson et al 2001).…”