Compact Optical Neural Probes With Up to 20 Integrated Thin-Film $\mu$LEDs Applied in Acute Optogenetic Studies

Ayub, Suleman; David, François; Klein, Eric; Borel, Mélodie; Oliver, Paul; Gentet, Luc J.; Ruther, Patrick

doi:10.1109/tbme.2020.2966293

Cited by 16 publications

(21 citation statements)

References 51 publications

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“…Furthermore, the maximal action potential (AP) response was always observed when the LED facing the neuron was turned on (Figure 2a). Therefore, light illumination was spatially-constrained to cortical depths directly facing or neighboring the stimulating LED, confirming our previous results (Ayub et al, 2020). classified as regular-spiking (RS) neurons based on their spike-shape (Figure 1e).…”

Section: Local and Global Inhibition By Pv Neurons In The Somatosensosupporting

confidence: 88%

“…We used two sets µLED array types (Ayub et al, 2020) that differed only by their inter-µLED distances (µLED pitch values of 150 or 250µm). Individual µLEDs were controlled using a custom-made Arduino-based driver.…”

Section: Optogenetic Stimulationmentioning

confidence: 99%

“…Here, we investigated the differential contribution of infra-versus supra-granular layer PV interneurons to the amplification of the oscillatory regimes of the mouse primary somatosensory barrel cortex (S1) via rhythmic and local activation of local PV-containing interneurons with arrays of miniaturized sources of light (µLEDssee Ayub et al, 2017Ayub et al, , 2020. In non-anaesthetized headfixed mice during quiet wakefulness in which spontaneous activity covers a large range of spontaneous oscillation frequencies (0.5 -100 Hz, Buzsáki and Draguhn, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Layer-specific stimulations of parvalbumin-positive cortical interneurons in mice entrain brain rhythms to different frequencies

David

Borel

Ayub

et al. 2021

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Neocortical interneurons provide local inhibition responsible for organizing neuronal activity into brain oscillations that subserve several functions such as memory, attention and neuronal communication. However, little is known about the contribution of interneurons to the entrainment of neocortical oscillations across cortical layers. Here, using layer-specific optogenetic stimulations with micro-Light-Emitting-Diode (μLED) arrays, directed toward parvalbumin-expressing (PV) interneurons in non-anesthetized awake mice, we find that supragranular layer stimulations of PV neurons were most efficient at entraining supragranular layer neurons and local field potential (LFP) oscillations at gamma frequencies (γ: 25 - 80 Hz), whereas infragranular layer stimulation of PV neurons better entrained delta (δ: 2 - 5 Hz) and theta (θ: 6 - 10 Hz) frequency LFP oscillations. We found that PV neurons tightly control the transmission of multiple rhythms to the network across cortical layers in an orientation-selective manner. Intrinsic resonant properties of neurons could underlie such layer-specific properties of rhythm entrainment.

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Section: Local and Global Inhibition By Pv Neurons In The Somatosensosupporting

confidence: 88%

Section: Optogenetic Stimulationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Layer-specific stimulations of parvalbumin-positive cortical interneurons in mice entrain brain rhythms to different frequencies

David

Borel

Ayub

et al. 2021

Preprint

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“…Using smaller diameter fibers (Eriksson et al, 2021) or incorporating waveguides (Schwaerzle et al, 2017) on the microfluidic probe would further reduce the brain damage but might come at the cost of higher optical losses and lower output power. Integration of light sources such as LEDs (Ayub et al, 2020;Wu et al, 2015) or laser diodes directly on the microfluidic probe would further enhance the versatility of our device and would facilitate scaling the system to multiple light sources while maintaining a small implant size.…”

Section: Discussionmentioning

confidence: 99%

Multifunctional optrode for opsin delivery, optical stimulation, and electrophysiological recordings in freely moving rats

Sharma

Jaeckel

Schneider

et al. 2021

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ObjectiveOptogenetics involves delivery of light-sensitive opsins to the target brain region, as well as introduction of optical and electrical devices to manipulate and record neural activity, respectively, from the targeted neural population. Combining these functionalities in a single implantable device is of great importance for a precise investigation of neural networks while minimizing tissue damage.ApproachWe report on the development, characterization, and in vivo validation of a multifunctional optrode that combines a silicon-based neural probe with an integrated microfluidic channel, and an optical glass fiber in a compact assembly. The silicon probe comprises an 11-μm-wide fluidic channel and 32 recording electrodes (diameter 30 μm) on a tapered probe shank with a length, thickness, and maximum width of 7.5 mm, 50 μm, and 150 μm, respectively. The size and position of fluidic channels, electrodes, and optical fiber can be precisely tuned according to the in vivo application.Main resultsWith a total system weight of 0.97 g, our multifunctional optrode is suitable for chronic in vivo experiments requiring simultaneous drug delivery, optical stimulation, and neural recording. We demonstrate the utility of our device in optogenetics by injecting a viral vector carrying a ChR2-construct in the prefrontal cortex and subsequent photostimulation of the transfected neurons while recording neural activity from both the target and adjacent regions in a freely moving rat. Additionally, we demonstrate a pharmacological application of our device by injecting GABA antagonist bicuculline in an anesthetized rat brain and simultaneously recording the electrophysiological response.SignificanceOur triple-modality device enables a single-step optogenetic surgery. In comparison to conventional multi-step surgeries, our approach achieves higher spatial specificity while minimizing tissue damage.Graphical abstract

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“…an optical ber surrounded by tetrodes 2 . This has been re ned with a single-tip approach 3 , integrated bers in electrode arrays 4 as well as using micro light -emitting diodes (µ-LEDs) combined with recording probes or co-integrated in silicon-based probes [5][6][7][8] . Here we focused on the optimization of optical bers as they allow targeting single cells as well as well as covering large volumes while keeping the exibility to apply any desired wavelength via an external exchangeable light source.…”

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confidence: 99%

Multichannel optogenetics combined with laminar recordings for ultra-controlled neuronal interrogation

Eriksson

Schneider

Thirumalai

et al. 2021

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Highlights: To combine large-scale recordings with optical perturbation we have developed a number of new techniques such as thin optical side-emitting fibers, a fiber matrix connector for thin fibers, an electro-optical commutator for multiple thin fibers, an active patch cord, a flexible fiber bundle ribbon cable, and a modular multi-optrode implantation holder.Summary: Simultaneous large-scale recordings and optogenetic interventions hold the promise to decipher the fast-paced and multifaceted dialogue between neurons that sustains brain function. Here we developed unprecedentedly thin, cell-sized Lambertian side-emitting optical fibers and combined them with silicon probes to achieve high quality recordings and ultrafast multichannel optogenetic inhibition in freely moving animals. Our new framework paves the way for large-scale photo tagging and controlled interrogation of rapid neuronal communication in any combination of brain areas.

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Compact Optical Neural Probes With Up to 20 Integrated Thin-Film $\mu$LEDs Applied in Acute Optogenetic Studies

Cited by 16 publications

References 51 publications

Layer-specific stimulations of parvalbumin-positive cortical interneurons in mice entrain brain rhythms to different frequencies

Layer-specific stimulations of parvalbumin-positive cortical interneurons in mice entrain brain rhythms to different frequencies

Multifunctional optrode for opsin delivery, optical stimulation, and electrophysiological recordings in freely moving rats

Multichannel optogenetics combined with laminar recordings for ultra-controlled neuronal interrogation

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