Community-acquired methicillin-resistant Staphylococcus aureus colonization in atopic dermatitis patients in Mansoura, Egypt

Abstract

Background: Atopic dermatitis (AD) patients are more frequently colonized with Staphylococcus aureus. The aim of this study is to determine the prevalence of community-acquired methicillin-resistant S. aureus (CA-MRSA) colonization in AD patients in a dermatology clinic in Mansoura, Egypt, and to assess the resistance pattern and the superantigen genes among isolated strains. Methods: Sixty AD patients were evaluated for the carriage of CA-MRSA in both lesional skin and nose. Antimicrobial susceptibility tests were performed using the disc diffusion method. The detection of mecA gene and superantigen genes, toxic shock syndrome toxin (tsst), and staphylococcal enterotoxin sea-sed were done by PCR. Results: Twenty-eight patients (46.7%) were colonized with CA-MRSA. All isolates were sensitive to vancomycin. Only 9.7% of isolates were resistant to ciprofloxacin, while 52.6% of isolates were resistant to fusidic acid and 48.4% were resistant to erythromycin. The predominant toxin gene found in AD patients was tsst that found in 100% of isolates of CA-MRSA, while seb and sed genes found only in 61.2% of the isolates. Conclusion: Atopic dermatitis patients were colonized with CA-MRSA. The most common toxin gene in the Egyptian AD patients was tsst. There is no significant association between any of superantigen genes and severity of AD.

Panton-Valentine Leukocidin (PVL) genes may not be a reliable marker for community-acquired MRSA in the Dakahlia Governorate, Egypt

“…A lower overall resistance rate of 6.85% was observed for ciprofloxacin. This confirms a previous report of a low resistance rate for CA-MRSA in Mansoura [ 41 ]. It seems that ciprofloxacin is not very commonly prescribed at Mansoura, and therefore, MRSA isolates are still highly sensitive to it.…”

Atopic dermatitis pediatric patients show high rates of nasal and intestinal colonization by methicillin-resistant Staphylococcus aureus and coagulase-negative staphylococci

“…In this study, MRSA isolates were found in 43.6% of AD patients in at least one of the clinical sites surveyed. Worldwide, rates of MRSA colonization in patients with AD have ranged from 16% to 57.4% [ 15 , 17 , 37 , 39 , 59 ]. For example, Ali et al (2019) [ 59 ] found MRSA isolates in 57.4% of lesioned skin and nares samples from AD patients attending clinics in Egypt.…”

“…Worldwide, rates of MRSA colonization in patients with AD have ranged from 16% to 57.4% [ 15 , 17 , 37 , 39 , 59 ]. For example, Ali et al (2019) [ 59 ] found MRSA isolates in 57.4% of lesioned skin and nares samples from AD patients attending clinics in Egypt. However, the prevalence of MRSA isolates can vary between countries and even within regions of the same country.…”