Commercial sugars as substrates for lipid accumulation in Cunninghamella echinulata and Mortierella isabellina fungi

Abstract

Cunninghamella echinulata and Mortierella isabellina, fungi capable of accumulating single cell oil (SCO) containing g-linolenic acid (GLA), were cultivated on sugar-based media, at initial substrate concentration 60 g/L. It was demonstrated that the carbon source plays an essential role on lipid accumulation process. Especially, on glucose, C. echinulata produced 3.9 g/L lipids containing 19.5% GLA, whereas M. isabellina achieved 9.9 g/L of lipid, containing 3.5% GLA. Growth on fructose was also satisfactory for both microorganisms. Although M. isabellina failed to grow adequately on saccharose, C. echinulata presented appreciable biomass production of 17.6 g/L, containing 18% of fat. Extra-cellular invertase activity was also detected in the case of C. echinulata cultures, achieving 0.5 U/mL. Both molds presented satisfactory growth on molasses, with significant amounts of SCO and GLA produced, accompanied by non-negligible substrate decolorization. Scalingup in a 3-L bioreactor of M. isabellina with glucose as a substrate resulted in 17.5 g/L of biomass, containing 12.7 g/L of lipid. Finally, fractionation of cellular lipid for both strains showed that neutral lipids represented about 90-92% w/w upon total accumulated lipids, whilst fractions of glycolipids plus sphingolipids and phospholipids were 6-7 and 3-4%, respectively.

Microbial oil production byMortierella isabellinafrom corn stover under different pretreatments

“…2.1.3 Aqueous ammonia pretreatment. Corn stover with the size of less than 2 mm was mixed with 10% NH 4 OH aqueous solution with a solid-to-liquid ratio of 1 : 10 (g:mL) and incubated in a shaker at 26 C for 24 h. Then it was ltered and washed with tap water 3 times. Subsequently, it was further treated with 0.3 mol L À1 HCl at 100-108 C for 1 h. 2.1.4 Lime pretreatment.…”

Comparative study of diesel engine performance and emission with soybean and waste oil biodiesel fuels

“…As food crops are not used to make second generation biodiesel, this type of fuel is more commonly used because it is a more efficient and viable option [40,41]. Third generation biodiesel is produced from microalgae [14,[42][43][44][45][46]. The research on biodiesel is important from the socio-economic and environmental points of view.…”

Reduction of Chemical and Biological Oxygen Demands from Oil Wastes via Oleaginous Fungi: An Attempt to Convert Food by Products to Essential Fatty Acids

“…Fungi and yeasts can grow on waste products, such as glycerol, which is a biodiesel byproduct (22). Hydrophilic materials and molasses have been applied to single Cunninghamella echinulata cells to make oil (23). Tomato hydrolyses waste was used by this fungi to produce fatty acids (15).…”