Comment: Practices for Drawing Blood Samples from Teleost Fish

Cooke, Steven J.; Lawrence, Michael; Raby, Graham D.; Teffer, Amy K.; Jeffries, Ken M.; Danylchuk, Andy J.

doi:10.1002/naaq.10115

Cited by 6 publications

(18 citation statements)

References 19 publications

(51 reference statements)

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“…First of all, we greatly appreciate Cooke et al. () for their comments on our work. Here, we will address their evaluation and concerns, and in doing so we hope to make a significant contribution to the science in this field.…”

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confidence: 95%

“…In addition, as was indicated by Cooke et al. (), drawing blood from arteries is critically important and should be detailed with figures and videos in new research.…”

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confidence: 99%

“…As stated by Cooke et al. (), blood can be drawn from a fish for various purposes. Physiological, biological, genetic, and stress studies need special emphasis regarding different application methods and routes (Nigam ).…”

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confidence: 99%

“…Cooke et al. () pointed out that drawing blood from living organisms is applied not only in disease cases but also for measuring many parameters. As was stated by Cooke et al.…”

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confidence: 99%

“…As was stated by Cooke et al. (), considering blood draws only for disease cases presents a narrow focus. However, by working on the methods of blood collection from fish, we (as aquatic animal disease professionals) tried to make it easier to eliminate the lack of information about the blood parameters of fish in disease cases.…”

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confidence: 99%

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Practices for Drawing Blood Samples from Teleost Fish: Response to Comment

et al. 2019

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confidence: 95%

“…In addition, as was indicated by Cooke et al. (), drawing blood from arteries is critically important and should be detailed with figures and videos in new research.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

“…Cooke et al. () pointed out that drawing blood from living organisms is applied not only in disease cases but also for measuring many parameters. As was stated by Cooke et al.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

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Practices for Drawing Blood Samples from Teleost Fish: Response to Comment

et al. 2019

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Best practices for non‐lethal blood sampling of fish via the caudal vasculature

Lawrence

Raby

Teffer

et al. 2020

Journal of Fish Biology

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Blood sampling through the caudal vasculature is a widely used technique in fish biology for investigating organismal health and physiology. In live fishes, it can provide a quick, easy and relatively non-invasive method for obtaining a blood sample (cf. cannulation and cardiac puncture). Here, a general set of recommendations are provided for optimizing the blood sampling protocol that reflects best practices in animal welfare and sample integrity. This includes selecting appropriate use of anaesthetics for blood sampling as well as restraint techniques for situations where sedation is not used. In addition, ideal sampling environments where the fish can freely ventilate and strategies for minimizing handling time are discussed. This study summarizes the techniques used for extracting blood from the caudal vasculature in live fishes, highlighting the phlebotomy itself, the timing of sampling events and acceptable blood sample volumes. This study further discuss considerations for selecting appropriate physiological metrics when sampling in the caudal region and the potential benefits that this technique provides with respect to long-term biological assessments. Although general guidelines for blood sampling are provided here, it should be recognized that contextual considerations (e.g., taxonomic diversity, legal matters, environmental constraints) may influence the approach to blood sampling. Overall, it can be concluded that when done properly, blood sampling live fishes through the caudal vasculature is quick, efficient and minimally invasive, thus promoting conditions where live release of focal animals is possible.

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Breeding Substrate Containing Distillation Residues of Mediterranean Medicinal Aromatic Plants Modulates the Effects of Tenebrio molitor as Fishmeal Substitute on Blood Signal Transduction and WBC Activation of Gilthead Seabream (Sparus aurata)

Antonopoulou,

Kolygas,

Panteli

et al. 2023

Preprint

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This work assesses the dietary use of two insect meals of Tenebrio molitor (TM) larvae reared in conventional (TM-10) or MAPs-enriched substrates (MAP-TM-10) as fish meal replacements (10%) in the diets of gilthead seabream (Sparus aurata). Fish (n=4,500; 207.19 ± 1.47 g) were divided into three groups with triplicates: Control (fed conventional diet), TM-10 and MAP-TM-10 groups.The fish were reared in floating cages for 12 weeks and The dietary effects on white blood cell ac-tivation, heat shock proteins, MAPKs, and apoptosis of the fish were evaluated. The MAP-TM-10 group exhibited the highest eosinophilic induction and plasma cells. Phosphorylated levels of p38 MAPK; p44/42 MAPK; HSP70 and HSP90 increased in the TM-10 and MAP-TM-10. In terms of apoptosis, Bax levels were lower in the TM groups compared to the Control, and the MAP-TM-10 group showed even lower levels than the TM-10 group. Bcl-2 levels increased in the TM-10 compared to the Control, and further increased in the MAP-TM-10 group. The Bax/Bcl-2 ratio, an apoptosis indicator, decreased in the TM groups, with the MAP-TM-10 group showing a further decrease compared to TM-10. These findings suggest that insects breeding substrate enriched with MAPs modulated the effect of TM on cellular stress and apoptosis.

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Comment: Practices for Drawing Blood Samples from Teleost Fish

Cited by 6 publications

References 19 publications

Practices for Drawing Blood Samples from Teleost Fish: Response to Comment

Practices for Drawing Blood Samples from Teleost Fish: Response to Comment

Best practices for non‐lethal blood sampling of fish via the caudal vasculature

Breeding Substrate Containing Distillation Residues of Mediterranean Medicinal Aromatic Plants Modulates the Effects of Tenebrio molitor as Fishmeal Substitute on Blood Signal Transduction and WBC Activation of Gilthead Seabream (Sparus aurata)

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