Comment on “Emerging Approaches to DNA Data Storage: Challenges and Prospects”

Armeni, Patrizio; Gatti, Anna

doi:10.1021/acsnano.2c11397

Cited by 1 publication

(2 citation statements)

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“…For 3 billion years, DNA has been used as a means of storing the information that characterizes an organism in distinct arrangements of the four bases (A, T, C, G). , When used as information carriers, DNA molecules outperform conventional storage media in many ways. It is a fantastic alternative for information storage and is anticipated to have widespread application in the future due to its high storage density, potential cheap maintenance cost, and other excellent properties. , …”

Section: Introductionmentioning

confidence: 99%

“…It is a fantastic alternative for information storage and is anticipated to have widespread application in the future due to its high storage density, potential cheap maintenance cost, and other excellent properties. 8,9 As DNA is an active biological nanomaterial, it is incredibly sensitive to the outside environment. 10 Reactive oxygen, ionizing radiation, heat, chemicals, and nuclease attacks are some of the factors that can lead to DNA degradation.…”

Section: Introductionmentioning

confidence: 99%

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Magnetic Bead Spherical Nucleic Acid Microstructure for Reliable DNA Preservation and Repeated DNA Reading

Shen

et al. 2023

ACS Synth. Biol.

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DNA is an attractive medium for long-term data storage because of its density, ease of copying, sustainability, and longevity. Recent advances have focused on the development of new encoding algorithms, automation, and sequencing technologies. Despite progress in these subareas, the most challenging hurdle in the deployment of DNA storage remains the reliability of preservation and the repeatability of reading. Herein, we report the construction of a magnetic bead spherical nucleic acid (MB-SNA) composite microstructure and its use as a cost-effective platform for reliable DNA preservation and repeated reading. MB-SNA has an inner core of silica@γ-Fe2O3@silica microbeads and an outer spherical shell of double-stranded DNA (dsDNA) with a density as high as 34 pmol/cm2. For MB-SNA, each strand of dsDNA stored a piece of data, and the high-density packing of dsDNA achieved high-capacity storage. MB-SNA was advantageous in terms of reliable preservation over free DNA. By accelerated aging tests, the data of MB-SNA is demonstrated to be readable after 0.23 million years of preservation at −18 °C and 50% relative humidity. Moreover, MB-SNA facilitated repeated reading by facile PCR-magnetic separation. After 10 cycles of PCR access, the retention rate of dsDNA for MB-SNA is demonstrated to be as high as 93%, and the accuracy of sequencing is more than 98%. In addition, MB-SNA makes cost-effective DNA storage feasible. By serial dilution, the physical limit for MB-SNA to achieve accurate reading is probed to be as low as two microstructures.

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