Combining platelet rich fibrin with different bone graft materials: An experimental study on the histopathological and immunohistochemical aspects of bone healing

Karayürek, Fatih; Kadiroğlu, Ela Tules; Nergız, Yusuf; Akçay, Neslihan Çoşkun; Tunik, Selcuk; Kanay, Berna Ersöz; Uysal, Ersin

doi:10.1016/j.jcms.2019.01.023

Cited by 26 publications

(21 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results are in concordance with those presented by Karayürek et al 7 where they observed that the combination of L-PRF with a Xenograft showed no significant benefit in new bone formation. This may be explained because the growth factors release occurs only during the first 7 days and subsequently the effect of L-PRF may not last enough to promote significant benefits in new bone formation.…”

Section: Discussionsupporting

confidence: 93%

“…[3][4][5][6] New generation platelet derivates, which claim to improve bone regeneration, are being used as an attempt give more biological properties and better results in these procedures. 7,8 Leukocyte and platelet rich fibrin (L-PRF), is an autologous platelet derivate, which liberates different grow factors, like platelet derived grow factor (PDGF), vascular endothelial grow factor (VEGF), transforming growth factor beta (TGF-β) and others. Growth factors are slowly liberated from the L-PRF matrix, and this liberation can last for up to seven days, which may improve tissue regeneration when used in https://doi.org/10.1016/j.jobcr.2020.05.001 Received 15 April 2020; Accepted 7 May 2020 T GBR.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Histological comparison of DBBM and platelet rich fibrin for guided bone regeneration in a rabbit model

Rezuc¹,

Saavedra

Maass

et al. 2020

Journal of Oral Biology and Craniofacial Research

View full text Add to dashboard Cite

To histologically evaluate the use of bovine derived deproteinized xenograft (DBBM), leukocyte and platelet rich fibrin (L-PRF) and the combination of both in Guided Bone Regeneration (GBR) performed in noncritical size defects in rabbit. Methods: A prospective experimental study was performed. Four bone defects in the tibiae of 12 rabbits were made and each of them was filled with DBBM, L-PRF, a combination of DBBM + L-PRF or was left to heal as control site. All defects were covered with a collagen membrane. Rabbits were randomly distributed in three groups and euthanatized at 3, 6 or 9 weeks. Samples were obtained and histologically analyzed to determine vital bone, connective tissue and remaining graft particles percentage. Analysis of variance, Kruskal Wallis and non-paired t-test where used to evaluate the significance of the results. Results: At 3 weeks of healing, DBBM showed significantly more vital bone percentage than L-PRF (p = 0,05) and DBBM + L-PRF showed significantly less connective tissue than control (p < 0,05). All other groups showed no statistical difference between them. At 6 weeks of healing, DBBM showed significantly more vital bone percentage than L-PRF (p < 0,05), DBBM + L-PRF (p < 0,05) and control (p < 0,05) and there wasn't any other significant difference regarding to connective tissue or remaining particle percentage between groups. At t 9 weeks healing period, there weren't any significant differences between groups. Conclusions: DBBM seems to enhance vital bone formation at early healing stages. The use of L-PRF alone or combined with DBBM, didn't show any histological improvement regarding to vital bone formation. The use of DBBM, alone or in conjunction with L-PRF showed a trend to reduce connective tissue percentage. The use of L-PRF combined with DBBM didn't affect the remaining particle percentage.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Histological comparison of DBBM and platelet rich fibrin for guided bone regeneration in a rabbit model

Rezuc¹,

Saavedra

Maass

et al. 2020

Journal of Oral Biology and Craniofacial Research

View full text Add to dashboard Cite

show abstract

“…Several authors have investigated the action of fibrin matrix in bone regeneration, either alone or in combination with other graft materials [18]. Platelet derivates have been used in bone regeneration to improve bone quality and bone quantity [19,20]. In fact, autologous platelet derivate, liberates different growth factors, such us vascular endothelial growth factor (VEGF), growth factor (PDGF) transforming growth factor beta (TGF-β), and others.…”

Section: Discussionmentioning

confidence: 99%

Role of Autologous Platelet Gel (APG) in Bone Healing: A Rabbit Study

et al. 2021

View full text Add to dashboard Cite

Purpose: The aim of the present study is to evaluate the influence and efficacy of autologous platelets on bone regeneration in a rabbit defects model. Materials and Methods: A total of 12 critical size tibial defects were produced in six New Zealand rabbits: A total of six defects were filled with autologous platelet gel (APG) and six defects were maintained as untreated controls. No membranes were used to cover the bone osteotomies. The histology and histomorphometry were performed at four weeks on retrieved samples of both groups. Results: No complications were reported in any of the animals nor for the defects produced. A significantly higher lamellar and woven bone percentage was reported for the APG group with a lower level of marrow spaces (p < 0.05). Evidence of newly formed bone was found in the superficial portion of the bone defect of APG samples where no aspects of bone resorption were observed. Conclusions: The evidence of the present research revealed that APG increases new bone formation restricted to the cortical portion and induces more rapid healing in rabbit bone defects than in untreated defects.

show abstract

“…Even if the combination of β-tricalcium-phosphate with PRF could not provide superiority in terms of bone regeneration, the immunohistochemical results showed a high expression of proliferation marker such as osteopontin and osteonectin. 59 Some studies investigated the effect of PRF on XBSM. 60 However, there is only low-level evidence of benefits of the combination of PRF with XBSM in terms of graft maturation when transferred in the clinical setting.…”

Section: Discussionmentioning

confidence: 99%

Combination of an allogenic and a xenogenic bone substitute material with injectable platelet-rich fibrin – A comparative in vitro study

et al. 2020

View full text Add to dashboard Cite

The aim of the in vitro study was a comparison of an allogenic (ABSM) and a xenogenic bone substitute material (XBSM) with and without injectable platelet-rich fibrin (ABSM-i-PRF & XBSM-i-PRF) on cell characteristics of human osteoblasts (HOB). Here, ABSM and XBSM (+ i-PRF = test; - i-PRF = control) were incubated with HOB for 3, 7 and 10 days. HOB viability, migration, proliferation and differentiation (RT-PCR on alkaline phosphatase (AP), bone morphogenetic protein 2 (BMP-2) and osteonectin (OCN)) were measured and compared between groups. At day 3, an increased viability, migration and proliferation was seen for ABSM-i-PRF. For viability and proliferation (days 7 and 10) and for migration (day 10), ABSM-i-PRF/XBSM-i-PRF showed higher values compared to ABSM/XBSM with maximum values for ABSM-i-PRF and minimum values for XBSM. At days 3 and 7, the highest expression of AP was detected in ABSM-i-PRF/XBSM-i-PRF when compared to ABSM/XBSM, whereas at day 10, AP expression levels were elevated in ABSM-i-PRF/ABSM. The highest BMP-2 expression was seen in ABSM-i-PRF whereas OCN expression showed higher levels in ABSM-i-PRF/XBSM-i-PRF at days 3 and 7 with lowest expression for ABSM. Later on, elevated OC levels were detected for ABSM-i-PRF only. In conclusion, i-PRF in combination with ABSM enhances HOB activity when compared to XBSM-i-PRF or untreated BSM in vitro. Therefore, addition of i-PRF to ABSM and – to a lower extent – to XBSM may influence osteoblast activity in vivo.

show abstract

Combining platelet rich fibrin with different bone graft materials: An experimental study on the histopathological and immunohistochemical aspects of bone healing

Cited by 26 publications

References 41 publications

Histological comparison of DBBM and platelet rich fibrin for guided bone regeneration in a rabbit model

Histological comparison of DBBM and platelet rich fibrin for guided bone regeneration in a rabbit model

Role of Autologous Platelet Gel (APG) in Bone Healing: A Rabbit Study

Combination of an allogenic and a xenogenic bone substitute material with injectable platelet-rich fibrin – A comparative in vitro study

Contact Info

Product

Resources

About