Combined venomics, venom gland transcriptomics, bioactivities, and antivenomics of two Bothrops jararaca populations from geographic isolated regions within the Brazilian Atlantic rainforest

Machado, Larissa Gonçalves; Plá, Davinia; Sanz, Libia; Jorge, Roberta Jeane Bezerra; Leitão-de-Araújo, Moema; Alves, Maria Lúcia Machado; Alvares, Diego Janisch; Miranda, Joari De; Nowatzki, Jenifer; Morais-Zani, Karen de; Fernandes, Wilson Roberto; Tanaka-Azevedo, Anita Mitico; Fernández, Julián; Zingali, Russolina B.; Gutiérrez, José Marı́a; Corrêa-Netto, Carlos; Calvete, Juan J.

doi:10.1016/j.jprot.2015.04.029

Cited by 116 publications

(86 citation statements)

References 91 publications

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“…Recent studies on the proteomic characterization of venoms are increasingly combining identification data with functional assays of particular components, to gain deeper insights from the medical and biological perspectives [57, 83–85]. The TS is conceptually identical to the ‘lethal neurotoxicity coefficient’ (LNC) defined as the ratio between the average LD 50 and the crotoxin + crotamine relative abundance (% of the total venom proteins) [50].…”

Section: Toxicovenomics: Unmasking the Villains Among The Crowdmentioning

confidence: 99%

Strategies in ‘snake venomics’ aiming at an integrative view of compositional, functional, and immunological characteristics of venoms

Lomonte

Calvete

2017

J Venom Anim Toxins Incl Trop Dis

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114

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This work offers a general overview on the evolving strategies for the proteomic analysis of snake venoms, and discusses how these may be combined through diverse experimental approaches with the goal of achieving a more comprehensive knowledge on the compositional, toxic, and immunological characteristics of venoms. Some recent developments in this field are summarized, highlighting how strategies have evolved from the mere cataloguing of venom components (proteomics/venomics), to a broader exploration of their immunological (antivenomics) and functional (toxicovenomics) characteristics. Altogether, the combination of these complementary strategies is helping to build a wider, more integrative view of the life-threatening protein cocktails produced by venomous snakes, responsible for thousands of deaths every year.

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Section: Toxicovenomics: Unmasking the Villains Among The Crowdmentioning

confidence: 99%

Strategies in ‘snake venomics’ aiming at an integrative view of compositional, functional, and immunological characteristics of venoms

Lomonte

Calvete

2017

J Venom Anim Toxins Incl Trop Dis

Self Cite

114

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“…Venomics is considered an integrative approach, that can combine proteomics, transcriptomics and/or genomics to study venoms [1]. Although the term was initially used to describe the mass spectrometry-based proteomic characterization of venoms [2,3], genomic [4,5] or more commonly venom gland transcriptomic sequencing [6][7][8][9][10][11][12][13][14] have also been used to characterize venom compositions. These molecular approaches provide an overview of venom composition by characterizing the nucleotide sequences of venom toxin-encoding genes (among others) and, in the case of transcriptomics, also provide an estimation of their relative expression in the venom gland.…”

Section: Introductionmentioning

confidence: 99%

Intact protein mass spectrometry reveals intraspecies variations in venom composition of a local population of Vipera kaznakovi in Northeastern Turkey

Petras

Hempel

Göçmen

et al. 2019

Journal of Proteomics

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“…In Argentina, the total area planted in row crops has increased rapidly in recent decades, coinciding with the expansion of soybean cultivation introduced in the mid 1970’s [10,11]. The expansion of soybean was facilitated by deforestation and replacement of natural systems, land use intensification, and changes in technologies, all which led to a homogenization of the landscape [10,12].…”

Section: Introductionmentioning

confidence: 99%

“…The expansion of soybean was facilitated by deforestation and replacement of natural systems, land use intensification, and changes in technologies, all which led to a homogenization of the landscape [10,12]. For example, planted area of soybean increased by more than 400% from 1985 to 2011, making Argentina one of the major soybean exporting countries in the world [11,13].…”

Section: Introductionmentioning

confidence: 99%

Multi-Season Regional Analysis of Multi-Species Occupancy: Implications for Bird Conservation in Agricultural Lands in East-Central Argentina

et al. 2015

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Rapid expansion and intensification of agriculture create challenges for the conservation of biodiversity and associated ecosystem services. In Argentina, the total row crop planted area has increased in recent decades with the expansion of soybean cultivation, homogenizing the landscape. In 2003 we started the first long-term, large-scale bird monitoring program in agroecosystems of central Argentina, in portions of the Pampas and Espinal ecoregions. Using data from this program, we evaluated the effect of land use and cover extent on birds between 2003-2012, accounting for imperfect detection probabilities using a Bayesian hierarchical, multi-species and multi-season occupancy model. We tested predictions that species diversity is positively related to habitat heterogeneity, which in intensified agroecosystems is thought to be mediated by food availability; thus the extent of land use and cover is predicted to affect foraging guilds differently. We also infer about ecosystem services provisioning and inform management recommendations for conservation of birds. Overall our results support the predictions. Although many species within each guild responded differently to land use and native forest cover, we identified generalities for most trophic guilds. For example, granivorous gleaners, ground insectivores and omnivores responded negatively to high proportions of soybean, while insectivore gleaners and aerial foragers seemed more tolerant. Habitat heterogeneity would likely benefit most species in an intensified agroecosystem, and can be achieved with a diversity of crops, pastures, and natural areas within the landscape. Although most studied species are insectivores, potentially beneficial for pest control, some guilds such as ground insectivores are poorly represented, suggesting that agricultural intensification reduces ecological functions, which may be recovered through management. Continuation of the bird monitoring program will allow us to continue to inform for conservation of birds in agroecosystems, identify research needed to reduce key uncertainties, and anticipate the effects of changes in agriculture in central Argentina.

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Combined venomics, venom gland transcriptomics, bioactivities, and antivenomics of two Bothrops jararaca populations from geographic isolated regions within the Brazilian Atlantic rainforest

Cited by 116 publications

References 91 publications

Strategies in ‘snake venomics’ aiming at an integrative view of compositional, functional, and immunological characteristics of venoms

Strategies in ‘snake venomics’ aiming at an integrative view of compositional, functional, and immunological characteristics of venoms

Intact protein mass spectrometry reveals intraspecies variations in venom composition of a local population of Vipera kaznakovi in Northeastern Turkey

Multi-Season Regional Analysis of Multi-Species Occupancy: Implications for Bird Conservation in Agricultural Lands in East-Central Argentina

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