Combined use of WEB2170 and HBO therapy can reduce ischemia and reperfusion injury to the skeletal muscle in a rabbit model

Abstract

Ischemia/reperfusion injury is regarded as the main cause of failure in revascularization of limbs and transfer of free flaps in the so called nonreflow phenomenon. This type of damage is caused by the production of free radicals, above all, of neutrophils that release great quantities of extracellular superoxide through the action of a membrane enzyme. In our study we used 40 white rabbits. Rabbit rectus femoris muscle is perfused by a single artery and vein and is therefore a valuable model for study of ischemia-induced reperfusion injury of skeletal muscle. The objective of this study was to individualize a valid method of protection for the muscle from damage by ischemia-induced reperfusion injury. We have tested the effectiveness of WEB2170, a PAF antagonist, of hyperbaric oxygen therapy one (HBO), and of combined employment of WEB2170 and HBO. The results show that both PAF and HBO play important protective roles against damage from ischemia/reperfusion injury, and that the combined employment of both therapies has a synergistic effect. We propose therefore a new therapeutic protocol for the prevention of damage resulting from ischemia/reperfusion injury with the simultaneous employment of this PAF and HBO.

Effect of Preconditioned Hyperbaric Oxygen and Ozone on Ischemia-Reperfusion Induced Tourniquet in Skeletal Bone of Rats

“…However, we focused on parameters of oxidative stress. There are many studies measuring just oxidative parameters in the literature [42,26]. In addition, HBO and ozone without IR groups should have studied, but the Ethics Committee of our institution (GATA Ethics Committee) did not allow studying more groups.…”

Treatment of Post-Revascularization Syndrome: brief communication

“…al., [1] proved a synergic effect of HBO and WEB2170, a platelet activating factor antagonist, association that decreases the infiltration of neutrophils in the ischemic tissue and reduces muscle damage during reperfusion after 3h and 30min of ischemia in the rectus femoris rabbit muscle.…”

“…Post-Revascularization Syndrome (PRS), also described as, Ischemic Reperfusion Injury (IRI), is the main cause of failure in the revascularization of limbs and the transfer of free flaps with 'non reflow' phenomenon [1]. It involves microcirculatory collapse during revascularization following an ischemic insult, that constitutes an acute inflammatory process by which cells are damaged first by temporary ischemia, hypoxia and accumulation of toxic metabolites and later by reperfusion [2,3].…”

“…The aim of the present review is to display the role of those treatments and their level of success, reporting the effective ones in reducing the extension of the injury caused by reperfusion and its aspects [1,2,3,5,6,7,8,11,12,13,15,16,17,18].…”

“…Already proved by Riccio et. al., [1] IRI it is the main cause of failure in the revascularization of limbs and the transfer of free flaps with the nonreflow phenomenon.…”