2010
DOI: 10.1186/1757-5036-3-14
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Abstract: The various lamellar phases of dipalmitoylphosphadtidylcholine bilayers with and without cholesterol were used to assess the versatility of the fluorescent probe merocyanine 540 through simultaneous measurements of emission intensity, spectral shape, and steady-state anisotropy. Induction of the crystalline phase (Lc') by pre-incubation at 4°C produced a wavelength dependence of anisotropy which was strong at 15 and 25°C, weak at 38°C, and minimal above the main transition (>~41.5°C) or after returning the tem… Show more

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Cited by 4 publications
(2 citation statements)
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“…Although dimer fluorescence would not be expected in a fluid phase, the data of Figure 6C suggested a continuous trend of incremental reduction in dimer fluorescence throughout the L o phase. Since at least some of the membranes of cells are expected to have properties analogous to a L o phase (lipid rafts) [48], this result of modest wavelength dependence of anisotropy in cells makes sense. Treatment of the cells with ionomycin altered both the anisotropy and emission intensity of MC540 in the samples (Figure 8A, B).…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Although dimer fluorescence would not be expected in a fluid phase, the data of Figure 6C suggested a continuous trend of incremental reduction in dimer fluorescence throughout the L o phase. Since at least some of the membranes of cells are expected to have properties analogous to a L o phase (lipid rafts) [48], this result of modest wavelength dependence of anisotropy in cells makes sense. Treatment of the cells with ionomycin altered both the anisotropy and emission intensity of MC540 in the samples (Figure 8A, B).…”
Section: Discussionmentioning
confidence: 99%
“…Gradual changes were also observed in the L β ' phase, although some of that result probably represents the breadth of the pre-transition. Fourth, cell plasma membranes are thought to possess characteristics reminiscent of a L o phase due to the presence of cholesterol and sphingomyelin-rich domains called rafts (reviewed in [48]). The data in Figure 8 suggest that this trait shifts toward behavior more like a liquid disordered phase when the cells are loaded with calcium as illustrated in Figure 9.…”
Section: Discussionmentioning
confidence: 99%