Combined transcriptomic, proteomic and biochemical approaches to identify the cadmium hyper-tolerance mechanism of turnip seedling leaves

Li, Xiong; Chen, Di; Li, Boqun; Yang, Ya; Yang, Yongping

doi:10.1007/s11356-020-11454-z

Cited by 12 publications

(20 citation statements)

References 84 publications

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“…The potential Cd-hyperaccumulator, turnip landrace KTRG-B14 [ 12 ], was used in this study, in which the protein acetylation/deacetylation processes were found to participate in Cd response [ 15 ]. The seeds were surface sterilized and germinated on petri dishes with double-layer filter papers, as previously described [ 14 ].…”

Section: Methodsmentioning

confidence: 99%

“…Fifteen micrograms of total protein from each sample were used for WB analysis, which was performed as previously described [ 15 ]. The polyvinylidene difluoride (PVDF) membranes were incubated with primary antibodies (anti-acetyllysine antibody, anti-succinyllysine antibody, and anti-crotonyllysine antibody: 1:1000 dilution) at 4 °C with gentle shaking overnight.…”

Section: Methodsmentioning

confidence: 99%

“…Previous studies found that the turnips cultivated in China had a generally high ability to tolerate and accumulate Cd [ 12 ]. The biochemical and molecular mechanisms of Cd tolerance and accumulation in turnips are of research interest and were recently explored using combined biochemical, transcriptomic, and proteomic approaches [ 13 , 14 , 15 ]. In addition to the conventional Cd transport and detoxification processes, these studies recently reported that the genes and/or proteins involved in the protein acetylation/deacetylation process were obviously induced, and that the overall acetylation modification level increased in turnip shoots under Cd stress [ 15 ], which suggests that protein post-translational modifications (PTMs), represented by acetylation in plants, likely play an important role in their response to Cd stress.…”

Section: Introductionmentioning

confidence: 99%

“…The lysine acylation modifications (e.g., acetylation, propionylation, butyrylation, succinylation, crotonylation, malonylation, and glutarylation) are common PTMs that often overlap with each other [ 19 , 20 ]. Our latest study indicated that the protein acetylation/deacetylation processes likely participated in the Cd response in the turnip leaves [ 15 ], and a previous study reported that oxidative stress triggered interactions between lysine acetylation and succinylation in rice leaves [ 19 ]. Oxidative stress is a major symptom of heavy metal toxicity in plants; this study thereby also indicated that acetylation and succinylation may be involved in the plant response to heavy metal stress.…”

Section: Introductionmentioning

confidence: 99%

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Quantitative Succinyl-Proteome Profiling of Turnip (Brassica rapa var. rapa) in Response to Cadmium Stress

Yang

et al. 2022

Cells

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Protein post-translational modification (PTM) is an efficient biological mechanism to regulate protein structure and function, but its role in plant responses to heavy metal stress is poorly understood. The present study performed quantitative succinyl-proteome profiling using liquid chromatography–mass spectrometry analysis to explore the potential roles of lysine succinylation modification in turnip seedlings in response to cadmium (Cd) stress (20 μM) under hydroponic conditions over a short time period (0–8 h). A total of 547 succinylated sites on 256 proteins were identified in the shoots of turnip seedlings. These succinylated proteins participated in various biological processes (e.g., photosynthesis, tricarboxylic acid cycle, amino acid metabolism, and response to stimulation) that occurred in diverse cellular compartments according to the functional classification, subcellular localization, and protein interaction network analysis. Quantitative analysis showed that the intensities of nine succinylation sites on eight proteins were significantly altered (p < 0.05) in turnip shoots after 8 h of Cd stress. These differentially succinylated sites were highly conserved in Brassicaceae species and mostly located in the conserved domains of the proteins. Among them, a downregulated succinylation site (K150) in the glycolate oxidase protein (Gene0282600.1), an upregulated succinylation site (K396) in the catalase 3 protein (Gene0163880.1), and a downregulated succinylation site (K197) in the glutathione S-transferase protein (Gene0315380.1) may have contributed to the altered activity of the corresponding enzymes, which suggests that lysine succinylation affects the Cd detoxification process in turnip by regulating the H2O2 accumulation and glutathione metabolism. These results provide novel insights into understanding Cd response mechanisms in plants and important protein modification information for the molecular-assisted breeding of Brassica varieties with distinct Cd tolerance and accumulation capacities.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Quantitative Succinyl-Proteome Profiling of Turnip (Brassica rapa var. rapa) in Response to Cadmium Stress

Yang

et al. 2022

Cells

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“…ABCG36 knockout promoted root Cd accumulation and potentiated Cd sensitivity in rice [45]. e YSL2 expression level was noticeably increased in the root and shoot of rice in response to Cd stress and YSL2 might be related Cd uptake transport [47,48]. ese transcripts might play vital roles in the response to Cd stress in rice.…”

Section: Bgiosga017856mentioning

confidence: 96%

Comparative Transcriptomic Analysis of Root Cadmium Responses in Two Chinese Rice Cultivars Yuzhenxiang and Xiangwanxian 12

ShangDu

Luo

et al. 2021

Journal of Chemistry

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Cadmium (Cd) pollution in paddy soil is an increasingly serious issue in rice production. It has been reported that there is a higher or lower grain Cd accumulation in the rice cultivars Yuzhenxiang (YZX) or Xiangwanxian 12 (XWX), respectively. To better manage the Cd pollution problem, the genes that might play vital roles in governing the difference in root Cd responses between these two rice cultivars were examined. In this study, the results of RNA sequencing (RNA-seq) showed that there were 341 and 161 differentially expressed genes in the roots of YZX and XWX after Cd exposure, respectively. Among these genes, 7 genes, such as Os06g0196300 (OsJ_019618), Os07g0570700 (OsJ_24808), ADI1, GDCSH, HSFB2C, PEX11-4, and CLPB1, possessed higher degree nodes with each other, through interaction analysis by the STRING (search tool for the retrieval of interacting genes/proteins) software, suggesting that they might play vital roles in Cd response. Based on GO enrichment analysis, 41 differently expressed genes after Cd treatment in YZX or XWX were identified to be related to Cd response. Through comparative transcriptomic analysis, 257 genes might be associated with the root Cd response difference between YZX and XWX. Furthermore, we supposed that ADI1, CFBP1, PEX11-4, OsJ_019618, OsJ_24808, GDCSH, CLPB1, LAC6, and WNK3 might be implicated in Cd response based on the combined analysis of RT-qPCR, interaction, and GO annotation analysis. In conclusion, the numerous genes that might be related to Cd stress response and root Cd response difference between YZX and XWX at the booting stage may be of benefit for the development of rice varieties with low Cd consumption.

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Polyaspartic acid enhances the Cd phytoextraction efficiency of Bidens pilosa by remolding the rhizospheric environment and reprogramming plant metabolism

Tian²,

Li³

et al. 2022

Chemosphere

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Combined transcriptomic, proteomic and biochemical approaches to identify the cadmium hyper-tolerance mechanism of turnip seedling leaves

Cited by 12 publications

References 84 publications

Quantitative Succinyl-Proteome Profiling of Turnip (Brassica rapa var. rapa) in Response to Cadmium Stress

Quantitative Succinyl-Proteome Profiling of Turnip (Brassica rapa var. rapa) in Response to Cadmium Stress

Comparative Transcriptomic Analysis of Root Cadmium Responses in Two Chinese Rice Cultivars Yuzhenxiang and Xiangwanxian 12

Polyaspartic acid enhances the Cd phytoextraction efficiency of Bidens pilosa by remolding the rhizospheric environment and reprogramming plant metabolism

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