Combined Effects of Fe3O4 Nanoparticles and Chemotherapeutic Agents on Prostate Cancer Cells In Vitro

Kojima, Kensuke; Takahashi, Sanai; Saito, Shungo; Endo, Yuichi; Nittami, Tadashi; Nozaki, Tadashige; Sobti, Ranbir Chander; Watanabe, Masatoshi

doi:10.3390/app8010134

Cited by 19 publications

(11 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This study demonstrated that Fe hours resulted in a signi cant inhibition of cell viability with different inhibitory effects [24]. Various studies have demonstrated that the mechanism of apoptosis by metal nanoparticles pertains to affecting ROS interactions in the treated cells [26 − 25].…”

Section: Discussionmentioning

confidence: 81%

Superparamagnetic Iron Oxide Nanoparticles Induce Apoptosis in HT-29 Cells by Increasing ROS and Damaging DNA

Ranjbary

Saleh

Azimi

2021

Preprint

View full text Add to dashboard Cite

Today, research into nanoparticles for diagnostic and therapeutic use in cancer is on the rise. In the present study, the effect of superparamagnetic iron oxide nanoparticles on the formation of apoptosis in HT-29 cells is investigated.In this study, we investigated the mechanisms of apoptosis induced by superparamagnetic iron oxide nanoparticles after MTT assay and determining the appropriate dose of 2.5 µg / mL to induce apoptosis in HT-29 cells.Superparamagnetic iron oxide nanoparticles increased the levels of ROS, Ca2 +, and DNA damage in HT-29 cells. Also, at the level of protein and mRNA, they increased the expression of Caspes 3 and 9 and significantly decreased Bcl-2 compared to the control group (P <0.0001).Fe3O4 causes apoptosis in cancer cells by increasing the level of ROS and intracellular calcium, followed by increasing the expression of caspases 3 and 9 and decreasing the expression of Bcl-2, as well as direct DNA damage.

show abstract

Section: Discussionmentioning

confidence: 81%

Superparamagnetic Iron Oxide Nanoparticles Induce Apoptosis in HT-29 Cells by Increasing ROS and Damaging DNA

Ranjbary

Saleh

Azimi

2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Yeh et al presented an H 2 O 2 -loaded ultrasound contrast agent H 2 O 2 /Fe 3 O 4 -poly(D,L-lactide-co-glycolic acid (PLGA) polymersome, which could yield sufficient • OH through the Fenton reaction of encapsulated H 2 O 2 and Fe 3 O 4 , completely removing the malignant tumors in a non-thermal process (Li et al, 2016 ). Watanabe et al investigated the combined effects of Fe 3 O 4 nanoparticles with chemotherapeutic agents (rapamycin or carboplatin) on prostate cancer cells in vitro (Kojima et al, 2018 ). Synergistic effect of Fe 3 O 4 NPs was observed in DU145 cells with carboplatin and in PC-3 cells with rapamycin, increasing intracellular ROS levels to decrease cancer cell viability significantly.…”

Section: Ferrite-based Ros-mediated Cancer Therapymentioning

confidence: 99%

“…Bahadur et al developed PEGylated mesoporous iron platinum-iron oxide composite nanoassemblies with high loading capacity of doxorubicin, which exhibited a higher efficiency of ROS generation compared to Fe 3 O 4 and Pt under the synergistic catalytic effect of FePt and Fe 3 O 4 , resulting in efficient chemo-and thermal therapy for Hela cancer cells (Sahu et al, 2015 (Li et al, 2016). Watanabe et al investigated the combined effects of Fe 3 O 4 nanoparticles with chemotherapeutic agents (rapamycin or carboplatin) on prostate cancer cells in vitro (Kojima et al, 2018). Synergistic effect of Fe 3 O 4 NPs was observed in DU145 cells with carboplatin and in PC-3 cells with rapamycin, increasing intracellular ROS levels to decrease cancer cell viability significantly.…”

Section: Intrinsic Fenton Reaction Of Ferritementioning

confidence: 99%

Ferrite Nanoparticles-Based Reactive Oxygen Species-Mediated Cancer Therapy

Zhang

et al. 2021

Front. Chem.

View full text Add to dashboard Cite

Ferrite nanoparticles have been widely used in the biomedical field (such as magnetic targeting, magnetic resonance imaging, magnetic hyperthermia, etc.) due to their appealing magnetic properties. In tumor acidic microenvironment, ferrite nanoparticles show intrinsic peroxidase-like activities, which can catalyze the Fenton reaction of hydrogen peroxide (H2O2) to produce highly toxic hydroxyl free radicals (•OH), causing the death of tumor cell. Recent progresses in this field have shown that the enzymatic activity of ferrite can be improved via converting external field energy such as alternating magnetic field and near-infrared laser into nanoscale heat to produce more •OH, enhancing the killing effect on tumor cells. On the other hand, combined with other nanomaterials or drugs for cascade reactions, the production of reactive oxygen species (ROS) can also be increased to obtain more efficient cancer therapy. In this review, we will discuss the current status and progress of the application of ferrite nanoparticles in ROS-mediated cancer therapy and try to provide new ideas for this area.

show abstract

“…These particles are coated with a biocompatible polymer to enhance their stability and cell transfer effects [15,16]. SPION-based drug delivery, nano-biomaterial, bio-imaging, and other methods are studied to determine their practical applications, and animal studies have been conducted to determine their potential in SCI therapy by using stem-cell-fusion to increase migration [17,18]. However, further studies are needed before SPIONs can be applied clinically and to demonstrate the migration, stability, and safety by magnetic induction of stem cell fusions [19,20].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Targeting and Imaging of Neurogenic Differentiation in Mouse Bone-Marrow Derived Mesenchymal Stem Cells with Superparamagnetic Iron Oxide Nanoparticles

et al. 2019

View full text Add to dashboard Cite

Spinal cord injuries (SCI) are well thought to be a crucial issue that roots various side effects for a patient during their entire lifetime. Although therapeutical methods to resolve the SCI are limited, stem cell therapy is determined to be a resolving factor since it possesses the ability to induce the neurogenic differentiation and the paracrine effect. However, stem cells are difficult to inject directly into the lesion, so they must be carefully guided through the spinal canal. Therefore, superparamagnetic iron oxide nanoparticles (SPIONs) are introduced as an instigator that makes the cells respond to the applied magnetic field. This study intends to report the synthesis strategy to develop SPIONs that could be used to treat the injury site by an applied magnetic field. SPION-internalized D1 Mesenchymal stem cells (MSCs) are observed consistently using a confocal fluorescence microscope to analyze the toxicity, maintenance, and monitoring points of intracellular SPIONs. The prepared SPIONs are much anticipated to increase the migration efficiency using magnetism, which was not cytotoxic. Hence, the prepared SPIONs can adeptly target the damaged neural tissue to promote tissue regeneration and treat nervous system disorders. This primary study stands as a focal point to solve SCI by stem cell migration effectively.

show abstract

Combined Effects of Fe3O4 Nanoparticles and Chemotherapeutic Agents on Prostate Cancer Cells In Vitro

Cited by 19 publications

References 38 publications

Superparamagnetic Iron Oxide Nanoparticles Induce Apoptosis in HT-29 Cells by Increasing ROS and Damaging DNA

Superparamagnetic Iron Oxide Nanoparticles Induce Apoptosis in HT-29 Cells by Increasing ROS and Damaging DNA

Ferrite Nanoparticles-Based Reactive Oxygen Species-Mediated Cancer Therapy

In Vitro Targeting and Imaging of Neurogenic Differentiation in Mouse Bone-Marrow Derived Mesenchymal Stem Cells with Superparamagnetic Iron Oxide Nanoparticles

Contact Info

Product

Resources

About