Combination of two oxidant stressors suppresses the oxidative stress and enhances the heat shock protein 27 response in healthy humans

Brerro-Saby, Christelle; Delliaux, Stéphane; Steinberg, Johannes; Boussuges, Alain; Gole, Yoann; Jammes, Yves

doi:10.1016/j.metabol.2009.10.006

Cited by 10 publications

(5 citation statements)

References 29 publications

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“…The lack of increase during exercise reflects previous observations demonstrating an increase in MDA only during exhaustive maximal exercise, as compared to exercise at 40% and 70% V Á o 2 max (Lovlin et al 1987). It has also been shown that eHsp27 concentration increases quickly in response to the combination of hyperoxia and maximal isometric exercise to fatigue, possibly to counterbalance the excessive production of reactive oxygen species (Wu 1995;Brerro-Saby et al 2010). Thus, the immuno-inflammatory release of eHsp27 occurs rapidly in venous blood after maximal exercise (Jammes et al 2009), which corroborates that noted in the current study.…”

Section: Discussionsupporting

confidence: 89%

“…During the exercise recovery period, eHsp72 may remain elevated to limit the response time to a pathogenic challenge . Although the precise role of eHsp27 is less well defined, increases in the extracellular concentration of eHsp27 are also associated with elevations in oxidative stress (Brerro-Saby et al 2010). An interesting finding was a reduced eHsp72 concentration 24 h following the cessation of exercise (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Accordingly, extracellular (eHsp27) levels increase markedly in the venous blood of healthy human subjects following maximal cycling exercise in thermoneutral conditions (Jammes et al 2009). Significant eHsp27 increases are also noted in response to exhaustive hand grip exercise under hyperoxic conditions (Brerro-Saby et al 2010), which indicates that both eHsp72 and eHsp27 expressions are upregulated under physiological stress.…”

Section: Introductionmentioning

confidence: 91%

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Plasma Hsp72 (HSPA1A) and Hsp27 (HSPB1) expression under heat stress: influence of exercise intensity

Périard

Ruell

Caillaud

et al. 2012

Cell Stress and Chaperones

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Extracellular heat-shock protein 72 (eHsp72) expression during exercise-heat stress is suggested to increase with the level of hyperthermia attained, independent of the rate of heat storage. This study examined the influence of exercise at various intensities to elucidate this relationship, and investigated the association between eHsp72 and eHsp27. Sixteen male subjects cycled to exhaustion at 60% and 75% of maximal oxygen uptake in hot conditions (40°C, 50% RH). Core temperature, heart rate, oxidative stress, and blood lactate and glucose levels were measured to determine the predictor variables associated with eHsp expression. At exhaustion, heart rate exceeded 96% of maximum in both conditions. Core temperature reached 39.7°C in the 60% trial (58.9 min) and 39.0°C in the 75% trial (27.2 min) (P<0.001). The rate of rise in core temperature was 2.1°C h −1 greater in the 75% trial than in the 60% trial (P<0.001). A significant increase and correlation was observed between eHsp72 and eHsp27 concentrations at exhaustion (P<0.005). eHsp72 was highly correlated with the core temperature attained (60% trial) and the rate of increase in core temperature (75% trial; P<0.05). However, no common predictor variable was associated with the expression of both eHsps. The similarity in expression of eHsp72 and eHsp27 during moderate-and high-intensity exercise may relate to the duration (i.e., core temperature attained) and intensity (i.e., rate of increase in core temperature) of exercise. Thus, the immuno-inflammatory release of eHsp72 and eHsp27 in response to exercise in the heat may be duration and intensity dependent.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 91%

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Plasma Hsp72 (HSPA1A) and Hsp27 (HSPB1) expression under heat stress: influence of exercise intensity

Périard

Ruell

Caillaud

et al. 2012

Cell Stress and Chaperones

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“…Indeed, most previous studies used western blotting to measure the HSP content in muscle biopsies and reported a significant HSP70 elevation in working muscle 20min (Locke et al, 1990) and 30min (Skidmore et al, 1995) after a treadmill bout in rats and 30min after an eccentric exercise in humans (Paulsen et al, 2007). ELISA measurements of plasma HSP27 and HSP70 content were performed in healthy humans and reported an early elevation of the phosphorylated HSP27 10min after maximal static handgrip (Brerro-Saby et al, 2010) and maximal cycling exercise ) while the phosphorylated HSP70 response began at 60min . The early increase in phosphorylated HSP content in tissues might result from their damage.…”

Section: Discussionmentioning

confidence: 99%

Fatiguing stimulation of one skeletal muscle triggers heat shock proteins activation in several rat organs: the role of muscle innervation

Jammes

Steinberg

et al. 2012

Journal of Experimental Biology

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SUMMARYWe hypothesised that activation of muscle afferents by fatigue triggers a widespread activation of heat shock proteins (HSPs) in resting muscles and different organs. In anaesthetised rats, HSP25 and HSP70 levels were determined in both tibialis anterior (TA) and extensor digitorum longus (EDL) muscles and in the diaphragm, kidney and brain by ELISA, which mostly identifies phosphorylated HSP, and western blotting. One TA muscle was electrically stimulated and tissues were sampled 10 or 60min after the stimulation had ended. The nerve supply to the stimulated TA or its counterpart in the contralateral limb was left intact or suppressed. In control rats, no muscle stimulation was performed and tissues were sampled at the same time points (10 or 60min). After TA stimulation, ELISA showed an increased HSP25 content in the contralateral TA, EDL and diaphragm at 10min but not at 60min, and HSP70 increased in all sampled tissues at 60min. Western blotting did not show any changes in HSP25 and HSP70 at 10min, while at 60min HSP25 increased in all sampled tissues except the brain and HSP70 was elevated in all tissues. Denervation of the contralateral non-stimulated limb suppressed HSP changes in TA and after denervation of the stimulated TA the widespread activation of HSPs in other organs was absent. Our data suggest that fatigue-induced activation of skeletal muscle afferents triggers an early increase in phosphorylated HSP25 in muscles and a delayed elevation of non-phosphorylated HSP25 and HSP70 in skeletal and respiratory muscles, kidney and brain.

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“…Of note, the Hsp27 levels significantly increased in response to the technical camp training only in the female players. It has been proposed that blood Hsp27 plays a direct role in protecting against the oxidative stress that is induced by exercise and hypoxia [ 39 ]. The physical workload that was imposed during the technical camp included more high-intensity exercise than that in the conditioning camp.…”

Section: Discussionmentioning

confidence: 99%

Planned Physical Workload in Young Tennis Players Induces Changes in Iron Indicator Levels but Does Not Cause Overreaching

Żurek

Lipińska

Antosiewicz

et al. 2022

IJERPH

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The current study aimed to examine the impact of the training load of two different training camps on the immunological response in tennis players, including their iron metabolism. Highly ranked Polish tennis players, between the ages of 12 and 14 years, participated in two training camps that were aimed at physical conditioning and at improving technical skills. At baseline and after each camp, blood samples were analyzed, and the fatigue was assessed. The levels of pro- and anti-inflammatory indicators, iron, and hepcidin were determined. The levels of the heat shock proteins, (Hsp) 27 and 70, were also measured. All the effects were evaluated using magnitude-based inference. Although the training camps had different objectives, the physiological responses of the participants were similar. The applied programs induced a significant drop in the iron and hepcidin levels (a small-to-very-large effect) and enhanced the anti-inflammatory response. The tumor necrosis factor α levels were elevated at the beginning of each camp but were decreased towards the end, despite the training intensity being medium/high. The changes were more pronounced in the female players compared to the male players. Altogether, the results suggest that low-grade inflammation in young tennis athletes may be attenuated in response to adequately designed training. To this end, the applied physical workload with a controlled diet and rest-controlled serum iron levels could be the marker of well-designed training.

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Combination of two oxidant stressors suppresses the oxidative stress and enhances the heat shock protein 27 response in healthy humans

Cited by 10 publications

References 29 publications

Plasma Hsp72 (HSPA1A) and Hsp27 (HSPB1) expression under heat stress: influence of exercise intensity

Plasma Hsp72 (HSPA1A) and Hsp27 (HSPB1) expression under heat stress: influence of exercise intensity

Fatiguing stimulation of one skeletal muscle triggers heat shock proteins activation in several rat organs: the role of muscle innervation

Planned Physical Workload in Young Tennis Players Induces Changes in Iron Indicator Levels but Does Not Cause Overreaching

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