Combination of oocyte and zygote selection by brilliant cresyl blue (BCB) test enhanced prediction of developmental potential to the blastocyst in cattle

Mirshamsi, S.M.; Karamishabankareh, Hamed; Ahmadi-Hamedani, M.; Soltani, Leila; Hajarian, Hadi; Abdolmohammadi, Alireza

doi:10.1016/j.anireprosci.2012.11.002

Cited by 25 publications

(34 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Thus, the authors correlated the abundance of transcripts of these genes with BCB + and BCB − oocytes to prove that the BCB method can be used to acquire more competent oocytes (Ashry et al, 2015). Moreover, the use of this colorimetric method in oocytes and zygotes increases the possibility of predicting the early embryonic development potential, since oocytes with blue cytoplasm and unstained zygotes significantly increase embryo production (Mirshamsi et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…Markers of oocyte quality were proposed in oocytes selected by BCB, with BCB + oocytes showing different gene expression profile (Ashry et al, 2015). Several authors (Alm et al, 2005;Wu, 2007;Mirshamsi et al, 2013) have evaluated selection of oocytes via BCB. In these studies, they concluded that oocytes could be stained by BCB without changing their competence and favorable results are obtained in selection of competent oocytes, when associated with morphological selection.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of seasonality on the quality of bovine oocytes selected by the brilliant cresyl blue method

Maia

Waltero

Marín³

et al. 2017

Rev Colomb Cienc Pecu

View full text Add to dashboard Cite

Background: The brilliant cresyl blue (BCB) staining is a non-invasive test to select the best-suited oocytes for embryonic development. This makes it a useful tool to select best-quality oocytes at the times of the year when there is forage restriction. Objective: To evaluate the effect of seasonality on the nuclear maturation and quality of oocytes selected by the BCB test. Methods: The cumulus-oophorus complexes (COCs) were obtained in summer and winter of 2010 and 2011. Selected COCs were maintained for 90 min at 38.5 °C in a CO 2 incubator, in TCM 199 medium containing 10% fetal bovine serum and antibiotics, and supplemented with 26 µM brilliant cresyl blue. Afterwards, they were divided according to the ooplasm staining (BCB + -blue; BCB − -unstained). Subsequently, COCs were matured for 22 h. Nuclear maturation was evaluated at 22 h of culture. Results: The proportion of BCB − oocytes was higher in the winter of 2010, but there was no increase in this group in the winter of 2011. The percentage of oocytes that reached metaphase II was higher in control and BCB + groups in relation to oocytes from BCB − group. Conclusion: The season of the year influences the percentage of oocytes best suited for embryonic production in situations in which

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of seasonality on the quality of bovine oocytes selected by the brilliant cresyl blue method

Maia

Waltero

Marín³

et al. 2017

Rev Colomb Cienc Pecu

View full text Add to dashboard Cite

show abstract

“…We found that the percentage of oocytes in which a clear protrusion cone was formed was higher ( p < 0.05) for BCB + than that for BCB -oocytes (77.1% vs. 62.0%, respectively). A higher blastocyst rate for BCB + than that for BCB -oocytes has been reported following IVF in many studies in cattle (Alm et al, 2005;Mirshamsi et al, 2013;Pujol et al, 2004;Silva et al, 2013) and buffalo (Manjunatha et al, 2007). It has been established through these studies that the level of G6PDH in the oocytes can be effectively used as a marker of their developmental competence following fertilization.…”

Section: Discussionmentioning

confidence: 99%

Buffalo Embryos Produced by Handmade Cloning from Oocytes Selected Using Brilliant Cresyl Blue Staining Have Better Developmental Competence and Quality and Are Closer to Embryos Produced byIn VitroFertilization in Terms of Their Epigenetic Status and Gene Expression Pattern

Mohapatra

Sandhu

Neerukattu

et al. 2015

Cellular Reprogramming

View full text Add to dashboard Cite

We compared handmade cloned (HMC) buffalo blastocysts produced from oocytes stained with Brilliant Cresyl Blue (BCB) and classified into those with blue (BCB + ) or colorless cytoplasm (BCB -). The blastocyst rate was higher ( p < 0.001) for BCB + than for BCB -oocytes (43.41 -2.54 vs. 22.74 -1.76%). BCB + blastocysts had inner cell mass (ICM) cell number, ICM-to-trophectoderm ratio, global level of H3K18ac, apoptotic index, and expression level of BCL-XL, but not that of CASPASE-3, similar to that of blastocysts produced through in vitro fertilization (IVF), which was higher ( p < 0.05) than that of BCB -blastocysts. The global level of H3K9me2, which was similar in BCB + and BCB -blastocysts, was higher ( p < 0.01) than that in IVF blastocysts. The expression level of OCT4 and SOX2 was higher ( p < 0.05) and that of GATA2 was lower ( p < 0.05) in BCB + than that in BCB -blastocysts, whereas that of DNMT1, DNMT3a, NANOG, and CDX2 was not significantly different between the two groups. The expression level of DNMT1, OCT4, NANOG, and SOX2 was lower ( p < 0.05) and that of CDX2 was higher ( p < 0.05) in BCB + than in IVF blastocysts. In conclusion, because BCB + blastocysts have better developmental competence and are closer to IVF blastocysts in terms of quality, epigenetic status, and gene expression than BCB -blastocysts, BCB staining can be used effectively for selection of developmentally competent oocytes for HMC.

show abstract

“…Em geral, vários grupos têm utilizado o ensaio ACB a fim de auxiliar a avaliação morfológica convencional na classificação de oócitos de melhor qualidade e, consequentemente, aumentar o sucesso da PIVE (MIRSHAMSI et al, 2013;OPIELA;KATSKA-KSIAZKIEWICZ, 2013). Normalmente, oócitos ACB + e com características morfológicas adequadas (considerados viáveis) possuem um melhor desempenho nas etapas de maturação e fecundação in vitro (MIRSHAMSI et al, 2013).…”

Section: Resultsunclassified

“…Normalmente, oócitos ACB + e com características morfológicas adequadas (considerados viáveis) possuem um melhor desempenho nas etapas de maturação e fecundação in vitro (MIRSHAMSI et al, 2013). Assim, alterações positivas ou negativas nos resultados de qualquer desses métodos de análise devem ser consideradas.…”

Section: Resultsunclassified

Influência Do Método De Recuperação Oocitária Sobre Os Parâmetros Quanti-Qualitativos De Oócitos Bovinos

Santos

Borges

Neta

et al. 2017

Ars Vet

View full text Add to dashboard Cite

RESUMOOs objetivos foram avaliar os parâmetros quanti-qualitativos de oócitos bovinos após recuperação usando diferentes métodos de colheita e tipos de êmbolo da seringa de aspiração. Assim, dois experimentos foram realizados usando ovários de fêmeas post-mortem. No primeiro experimento, duas técnicas de colheita oocitária foram empregadas: aspiração de folículos (2-8 mm) com agulha 21G e seringa de 5 mL e, fatiamento da superfície ovariana (slicing). No segundo experimento, folículos (2-8 mm) foram aspirados usando seringa com distintos êmbolos (borracha vs. plástico). Os complexos cumulus-oócito (CCOs) foram classificados por critérios morfológicos em viáveis e não viáveis. Em seguida, CCOs foram corados com o azul de cresil brilhante (ACB) (60 min; 26 μM) e classificados como ACB + (viáveis) e ACB -(não viáveis). Um total de cinco repetições por experimento foi realizado e os dados analisados pelo teste exato de Fisher (P<0,05). No primeiro experimento, o número de oócitos por ovário obtido por slicing foi maior quando comparado à aspiração folicular (14,8 vs. 7,5; P<0,05). Contudo, um maior percentual de CCOs viáveis observado pelo ensaio de ACB foi obtido a partir da aspiração folicular (65,7% vs. 31,0%; P<0,05). No segundo experimento, nenhuma diferença (P>0,05) foi observada entre os tipos de êmbolos quanto aos parâmetros quantitativos. Quanto à qualidade oocitária avaliada por critérios morfológicos, um percentual maior de oócitos viáveis foi recuperado usando êmbolo de borracha (75,4% vs. 58,2%; P<0,05). Em conclusão, oócitos de melhor qualidade podem ser obtidos a partir da aspiração folicular, especialmente usando seringa com êmbolo de borracha. PALAVRAS-CHAVE:Aspiração folicular. Azul de cresil brilhante. Colheita oocitária. Qualidade oocitária. Slicing. SUMMARYThe aims were to evaluate qualitative-quantitative parameters of bovine oocytes after recovery using different collection methods and piston type from the aspiration syringe. Thus, two experiments were performed using ovaries from postmortem females. In the first experiment, two techniques of oocyte recovery were used: aspiration of follicles (2-8 mm) with 21G needle and 5 mL syringe, and slicing of the ovarian surface. In the second experiment, follicles (2-8 mm) were aspirated using syringes with different pistons (rubber vs. plastic). The cumulus-oocyte complexes (COCs) were classified by morphological criteria as viable and non-viable. Then, COCs were stained with brilliant cresyl blue (BCB) (60 min, 26 µM) and categorized as BCB + (viable) and BCB -(non-viable). A total of five repetitions per experiment was performed and the data analyzed by Fisher's exact test (P<0.05). In the first experiment, the number of oocytes per ovary obtained by slicing was higher when compared to the follicular aspiration (14.8 vs. 7.5; P<0.05). Nevertheless, a greater percentage of viable COCs observed by BCB assay was obtained from the follicular aspiration (65.7% vs. 31.0%; P<0.05). In the second experiment, no difference (P>0.05) was observed the piston ty...

show abstract

Combination of oocyte and zygote selection by brilliant cresyl blue (BCB) test enhanced prediction of developmental potential to the blastocyst in cattle

Cited by 25 publications

References 24 publications

Effect of seasonality on the quality of bovine oocytes selected by the brilliant cresyl blue method

Effect of seasonality on the quality of bovine oocytes selected by the brilliant cresyl blue method

Buffalo Embryos Produced by Handmade Cloning from Oocytes Selected Using Brilliant Cresyl Blue Staining Have Better Developmental Competence and Quality and Are Closer to Embryos Produced byIn VitroFertilization in Terms of Their Epigenetic Status and Gene Expression Pattern

Influência Do Método De Recuperação Oocitária Sobre Os Parâmetros Quanti-Qualitativos De Oócitos Bovinos

Contact Info

Product

Resources

About