Combination of Mussel Inspired Method and “Thiol-Michael” Click Reaction for Biocompatible Alginate-Modified Carbon Nanotubes

Yao, Haiyan; Zhu, Mingzhi; Wang, Pei; Liu, Yuangang; Wei, Junchao

doi:10.3390/nano11092191

Cited by 5 publications

(4 citation statements)

References 38 publications

(40 reference statements)

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“…No significant differences were found between the two nanocarriers. These results are consistent with previous data for CNT, CNT-modified polydopamine, and CNT-modified alginate on MC3T3-E1 and HepG2 cells, demonstrating that the cytocompatibility is concentration-dependent . The obtained results may indicate that cytocompatibility relies on the concentration of CNT used as the drug nanocarrier. , …”

Section: Resultssupporting

confidence: 92%

Functionalized Carbon Nanotubes for Delivery of Ferulic Acid and Diosgenin Anticancer Natural Agents

AbouAitah,

Abdelaziz,

Higazy

et al. 2024

ACS Appl. Bio Mater.

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It was investigated whether loading multi-wall carbon nanotubes (CNTs) with two natural anticancer agents: ferulic acid (FUA) and diosgenin (DGN), may enhance the anticancer effect of these drugs. The CNTs were functionalized with carboxylic acid (CNTCOOH) or amine (CNTNH2), loaded with the above pro-drugs, as well as both combined and coated with chitosan or chitosan–stearic acid. Following physicochemical characterization, the drug-loading properties and kinetics of the drug’s release were investigated. Their effects on normal human skin fibroblasts and MCF-7 breast carcinoma cells, HepG2 hepatocellular carcinoma cells, and A549 non-small-cell lung cancer cells were evaluated in vitro. Their actions at the molecular level were evaluated by assessing the expression of lncRNAs (HULC, HOTAIR, CCAT-2, H19, and HOTTIP), microRNAs (mir-21, mir-92, mir-145, and mir-181a), and proteins (TGF-β and E-cadherin) in HepG2 cells. The release of both pro-drugs depended on the glutathione concentration, coating, and functionalization. Release occurred in two stages: a no-burst/zero-order release followed by a sustained release best fitted to Korsmeyer–Peppas kinetics. The combined nanoformulation cancer inhibition effect on HepG2 cancer cells was more pronounced than for A549 and MCF7 cells. The combined nanoformulations had an additive impact followed by a synergistic effect, with antagonism demonstrated at high concentrations. The nanoformulation coated with chitosan and stearic acid was particularly successful in targeting HepG2 cells and inducing apoptosis. The CNT functionalized with carboxylic acid (CNTCOOH), loaded with both FUA and DGN, and coated with chitosan–stearic acid inhibited the expression of lncRNAs and modulated both microRNAs and proteins. Thus, nanoformulations composed of functionalized CNTs dual-loaded with FUA and DGN and coated with chitosan–stearic acid are a promising drug delivery system that enhances the activity of natural pro-drugs.

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Section: Resultssupporting

confidence: 92%

Functionalized Carbon Nanotubes for Delivery of Ferulic Acid and Diosgenin Anticancer Natural Agents

AbouAitah,

Abdelaziz,

Higazy

et al. 2024

ACS Appl. Bio Mater.

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“…Accumulating evidence suggested that the morphology and size of nanomaterials play a critical role in cell behaviors, including adhesion, viability, proliferation, migration, functionalization or differentiation ( Nguyen et al, 2016 ; Zhou et al, 2018 ; Li et al, 2020 ; Liu M. et al, 2021 ; Wang et al, 2021b ; Yang et al, 2021 ; Yao et al, 2021 ). Figure 2A shows that the nanosized HA particles possessed uniform distribution and needle-shaped morphology.…”

Section: Resultsmentioning

confidence: 99%

Regulation of T Cell Responses by Nano-Hydroxyapatite to Mediate the Osteogenesis

Guo

Yuan

Huang

et al. 2022

Front. Bioeng. Biotechnol.

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Nano-hydroxyapatite (nHA) has been widely applied as a tissue-engineering biomaterial and interacted with osteoblasts/stem cells to repair bone defects. In addition, T cells that coexist with osteoblasts/stem cells in the bone modulate the regulation of osteoimmunology by cytokine formation. However, the effects of nHA on T cells and the following regulatory interplay on osteogenic differentiation have been rarely examined. In this work, the physicochemical properties of needle-like nHA are characterized by field emission scanning electron microscopy, zeta potential, Fourier transform-infrared and X-ray diffraction. It is found that as the concentration of nHA increases, the proliferation of T cells gradually increases, and the proportion of apoptotic T cells decreases. The percentage of CD4+ T cells is higher than that of CD8+ T cells under the regulation of needle-like nHA. Furthermore, the supernatant of T cells co-cultured with nHA significantly inhibits the osteogenic differentiation of MC3T3-E1 by downregulating the formation of alkaline phosphatase and calcium nodule compared with the supernatant of nHA. Thus, our findings provide new insight into the nHA-mediated T cell and osteoblast interactions.

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“…The molecular weight of alginate, measured at ≈ 67 kDa, enabled filter sterilization of the precursors and, therefore, easy to use for 3D cell culture applications. In the scientific literature, several systems have been developed for the formation of alginate hydrogels by covalent cross-linking, including the thiol-ene reaction [32] or the inverse electron-demand Diels-Alder (IEDDA) reaction [33], [34]. However, these reactions have certain drawbacks that limit their use in the biological and medical fields.…”

Section: Discussionmentioning

confidence: 99%

Microencapsulation of mesenchymal stromal cells in covalent alginate hydrogels for cell therapy

Ambrosino,

Nativel,

Boyer

et al. 2023

Preprint

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Osteoarthritis (OA) is the most common inflammatory joint disease and currently lacks an effective curative treatment. Intra-articular injection of mesenchymal stromal cells (MSCs) has gained attention as a relevant therapeutic approach for OA treatment due to the MSC’s ability to secrete anti-inflammatory and immunomodulatory factors. Given their limited viability post-intraarticular injection and the potential leakage of cells out of the injection site, encapsulating MSCs in hydrogels is considered a promising strategy to protect them and provide a suitable 3D microenvironment to support their biological activities. Calcium-cross-linked alginate hydrogels are commonly used for MSC encapsulation, but their long-term in vivo stability remains uncertain. On the other hand, alginate cross-linking by the strain-promoted azide-alkyne cycloaddition (SPAAC) reaction would create a network unaffected by an ionic environment. Hence, this study aimed to develop an alginate-based hydrogel cross-linked via stable and cytocompatible covalent bonds for cell encapsulation. We established for the first time the formation of covalent alginate hydrogels between two SPAAC precursors, namely alginate-BCN and alginate-N3. These hydrogels exhibited in vitro stability and enabled the diffusion of molecules of interest. We then generated alginate-based SPAAC microgels of 170 μm in mean diameter, suitable for intra-articular injection. We next encapsulated human adipose MSCs (hASCs) in these alginate-based SPAAC microgels and confirmed their cytocompatibility, with over 90 % of cells remaining viable after 14 days in culture. Finally, the microencapsulated hASCs maintained their biological properties and were able to secrete anti-inflammatory factors (IDO, PGE2, and HGF) when exposed to pro-inflammatory cytokines (TNF-α and IFN-γ). In the end, human activated lymphocytes were cultured in contact with microencapsulated hASCs, and CD3+ T cell proliferation was quantified by flow cytometry. We demonstrated that the encapsulation process did not impair the hASC immunomodulatory activity. Overall, our findings show the potential of alginate-based SPAAC hydrogels for microencapsulating hASCs for cell therapy.

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Combination of Mussel Inspired Method and “Thiol-Michael” Click Reaction for Biocompatible Alginate-Modified Carbon Nanotubes

Cited by 5 publications

References 38 publications

Functionalized Carbon Nanotubes for Delivery of Ferulic Acid and Diosgenin Anticancer Natural Agents

Functionalized Carbon Nanotubes for Delivery of Ferulic Acid and Diosgenin Anticancer Natural Agents

Regulation of T Cell Responses by Nano-Hydroxyapatite to Mediate the Osteogenesis

Microencapsulation of mesenchymal stromal cells in covalent alginate hydrogels for cell therapy

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