Combination of Ficus Carica Leaves Extract and Ubiquinone in a Chronic Model of Lithium Induce Reproductive Toxicity in Rats: Hindrance of Oxidative Stress and Apoptotic Marker of Sperm Cell Degradation

Haredy, Shimaa A.; Imam, Tamer S.; Ahmed‐Farid, Omar A.

doi:10.9790/3008-1202026473

Cited by 7 publications

(7 citation statements)

References 29 publications

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“…The decrease in MDA levels and the increase in antioxidant enzyme activity in the extract treatment group in this study indicated that the extract contained antioxidant secondary metabolites. The results of this study are in accordance with those of Haredy et al [45] who documented that the activity of antioxidant enzymes in the seminiferous tubules was significantly increased by F. carica treatment. This is also supported by the results of Omar et al [46] that found that F. deltoidea efficacy is attributed to its high phenolic and flavonoid content.…”

Section: Resultssupporting

confidence: 93%

Effects of the Ethanol Extracts of Ficus deltoidea leaves on the Reproductive Parameters in Male Mice

Nugroho

Aryani

Manurung

et al. 2022

Open Access Maced J Med Sci

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BACKGROUND: Indonesia, and in particular East Kalimantan, has a very high diversity of flora that has the potential to be used as traditional medicine. One type of flora is the leaves of Ficus deltoidea Jack. At present, there are no available data about the impact of F. deltoidea leaf ethanol extract on the male reproductive system. AIM: The present study aims to investigate the effect of F. deltoidea leaf ethanol extract on several parameters of reproductive function in male mice, including changes in testicular biochemistry, reproductive hormones profile, and histopathology of the testes after subchronic exposure. METHODS: In total, 25 male mice were divided into five groups: The control group and four treatment groups that received extract doses of 125, 250, 500, and 1000 mg/kgbw for 28 days, respectively. At the end of the treatment, surgery was performed, weight of body and reproductive organs (testis, epididymis, and seminal vesicles) were measured, and testicular biochemistry, reproductive hormone profile, antioxidant activity, and testes histopathology were analyzed. RESULTS: In the subchronic toxicity test, there were no significant changes in body weight or in weight and relative weight of reproductive organs. Levels of testosterone, luteinizing hormone and follicle-stimulating hormone, protein, cholesterol, and activity of enzymes in the testes (alkaline phosphatase, lactate dehydrogenase, and glutamyltransferase) and activity of the enzyme superoxide dismutase increased significantly in the treated mice when compared to control mice (p < 0.05). Glycogen levels were not significantly different, but lipid peroxide (MDA) decreased significantly, though it did not change the histological structure of the testes. CONCLUSION: Ethanolic extract of the leaves of F. deltoidea Jack does not cause toxic effects and even has a beneficial effect on the reproduction of male mice by increasing fertility, reproductive hormones, and antioxidant activity, and it does not change the histological structure of the testes.

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Section: Resultssupporting

confidence: 93%

Effects of the Ethanol Extracts of Ficus deltoidea leaves on the Reproductive Parameters in Male Mice

Nugroho

Aryani

Manurung

et al. 2022

Open Access Maced J Med Sci

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“…F. carica increased the levels of FSH more than that of LH, DHT, and estrogen which is therefore responsible for the increase in fertility in parent males. The outcomes of the present study are in accordance with a previous study by Haredy et al [ 19 ], who reported a reduction in lithium carbonate-induced liver toxicity by F. carica and increased endogenous activity of antioxidant enzymes (CAT and SOD2) and semen quality, while there was a significant decrease in markers of oxidative stress in testes, i.e., NO, 8OHdG, MDA and GSSG. Thus, it may be concluded that increased gene expression tends to reduce oxidative stress and in turn increases overall reproductive activity.…”

Section: Discussionsupporting

confidence: 93%

The Effects of Ficus carica on Male and Female Reproductive Capabilities in Rats

ain

Khan

Mirza

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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The basic purpose of pharmacology is to look into the benefits of natural remedies and make them available to the general populace. Herbal medicines are now considered to be the future of humanity. The current study explored the effects of Ficus carica (FC) extract in rats of two-generation F0 (parents) and F1 (offspring) in either sex. The F. carica extract was initially tested for acute and sub-chronic toxicity. Extracts were also tested for fertility assessment and effects on reproductive hormones like follicle-stimulating hormone (FSH), luteinizing hormone (LH), gonadotropin-releasing hormone (GnRH), estradiol, testosterone, dihydrotestosterone (DHT), dehydroepiandrosterone-sulfate (DHEAS), insulin, progesterone, and prolactin. The antioxidant activity of the extract was also evaluated by testing mRNA expressions of SOD2, GPX1, CAT, and GR in male testes and female ovaries. The animals treated with 100 mg/kg FC extract produced a more pronounced fertility effect in both genders. Expression of CAT, SOD2, GPX1, and GR was found to be increased in the reproductive organs of both sexes. Histology of the testes reveals increased spermatogenesis and increased folliculogenesis in ovaries. The hormone profile showed an increase in FSH, DHT, estradiol, and DHEAS levels in males and FSH, LH, estrogen, and DHEAS in females. The results of the study establish the effectiveness of natural products in improving fertility issues in either sex.

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“…Phytoestrogens are compounds found in plants that have a similar structure and function of estrogen (Price and Rhodes, 1997). Also, the improvement of SSCs` proliferation in the presence of FC leaf extract may be due to FC containing saponin that act as testosterone like action (Haredy et al, 2017). In addition to estrogen, Tajik et al (2012) reported the involvement of testosterone in SSCs enrichment in the culture.…”

Section: Discussionmentioning

confidence: 99%

“…Also, after administration of FC leaf extract, spermatogenic arrest was rarely seen in seminiferous tubule of testis exposed to formaldehyde. Based on a compilation by Haredy et al, FC ameliorated Lithium carbonate -induced reduction in reproductive performance of male rats through its antioxidant and antiapoptotic properties (Haredy et al, 2017). A recent study also reported the profertility effect of FC leaf extract on streptozotocin-induced diabetic male rats.…”

Section: Introductionmentioning

confidence: 98%

Efficacy of Ficus carica leaf extract on morphological and molecular behavior of mice germ stem cells

Makoolati

Bahrami²,

Zamanzadeh³

et al. 2022

Anim. Reprod.

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Infertility is one of the most prevalent health disorders in reproductive-age males and females. Ficus carica (Fc), an herbal plant, has been used traditionally for the treatment of different diseases such as infertility especially in Iranian folk medicine. This study examined the effects of Fc leaf extract on the proliferation of mice spermatogonial stem cells (SSCs). Phenolic, flavonoid content, major polyphenolic compounds and antioxidant activity of the extract was evaluated respectively by Folin-Ciocateu, aluminum chloride, HPLC and the FRAP and DPPH methods. Testicular cells of neonate mice were extracted and their identity was confirmed using cytokeratin for Sertoli and Oct-4, CDHI and PLZF for SSCs. Effects of Fc (0.0875, 0.175, 0.35, 0.71 and 1.42 mg/ml) was evaluated at third, 7th, 9th and 14th days of culture by colony assay. The expression of the Mvh, GFRα1 and Oct-4 genes and the viability and proliferation of cultured cells was assessed at the end of the culture period. The extract has a rich phenolic and flavonoid content such as Rutin, Psoralen, Bergapten and Caffeoylmalic acid using HPLC analysis. It also had a potent reducing and radical scavenging activity. Morphology of colonies was similar in all groups. Higher viability, proliferation, colony number and diameter of SSCs was seen in the presence of Fc leaf extract in a dosedependent manner so that higher number and diameter of colonies were observed in two higher doses of 0.71 and 1.42 mg/ml, separately for each time point relative to other groups. The Mvh, Oct-4 and GFRα1 genes expression had no significant differences between groups. It seems that Fc leaf extract not only had no any cytotoxic effects on the viability and proliferation of SSCs but also support their stemness state. So, this culture system can be employed for enrichment of germ stem cells for use in clinical applications.

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Combination of Ficus Carica Leaves Extract and Ubiquinone in a Chronic Model of Lithium Induce Reproductive Toxicity in Rats: Hindrance of Oxidative Stress and Apoptotic Marker of Sperm Cell Degradation

Abstract: groups treated with 25 mg /kg body weight of Lithium carbonate, FE (200mg/kg b.w) and CoQ10 (20 mg/kg b.w) (FC+CoQ10+LC). The experiment lasted for 60 days. Revealed data showed that the LC induces significant (P>0.05) increase in serum ALT, AST level, testicular GSSG, NO,

Cited by 7 publications

References 29 publications

Effects of the Ethanol Extracts of Ficus deltoidea leaves on the Reproductive Parameters in Male Mice

Effects of the Ethanol Extracts of Ficus deltoidea leaves on the Reproductive Parameters in Male Mice

The Effects of Ficus carica on Male and Female Reproductive Capabilities in Rats

Efficacy of Ficus carica leaf extract on morphological and molecular behavior of mice germ stem cells

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