Combination of cafeteria diet with intraperitoneally streptozotocin in rats. A type-2 diabetes model

Santos, Mara Lisiane dos; Cavalcante, Andressa Carolina Farias Pereira Subtil; Amaral, Luane Aparecida do; Souza, Gabriel Henrique Oliveira de; Santos, Bárbara Suzuki Dos; Portugal, Luciane Candeloro; Bittencourt, Felipe Francisco; Troquez, Thiago; Rafacho, Bruna Paola Murino; Hiane, Priscila Aiko; Santos, Elisvânia Freitas dos

doi:10.1590/acb360702

Cited by 3 publications

(1 citation statement)

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“…After 10 days, the animals received an intraperitoneal injection of streptozotocin (40 mg·kg –1 ; Sigma Chemical, St. Louis, MO, USA) dissolved in a 20 mmol·L –1 sodium citrate solution (pH = 4.5). The streptozotocin dosage performed in this study for the experimental model of type 2 diabetes used, simulates metabolic characteristics of DM2 in humans 16 . Blood glucose was measured 48 h after the injection, and fasting glycemia > 250 mg·dL –1 was considered hyperglycemia.…”

Section: Methodsmentioning

confidence: 99%

Analysis of high-intensity interval training on bone mineral density in an experimental model of type 2 diabetes

et al. 2022

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Purpose: To analyze the effect of high-intensity interval training (HIIT) on bone mineral density (BMD) in a model of type 2 diabetes mellitus. Methods: Thirty-two male, adult, 12-week-old rats ( Rattus norvegicus ), of the Wistar lineage, were used. The animals induced to the experimental model received a high fat diet for 10 days and, after that period, intraperitoneal injection of streptozotocin (40 mg·kg –1 ), dissolved in 20 mmol·L –1 sodium citrate solution (pH = 4.5). The experimental group of diabetes was formed by the animals that, 48 h after the injection of streptozotocin, had fasting blood glucose > 250 mg·dL –1 ). The animals were randomly divided into four groups with eight animals each: HIIT experimental diabetes; HIIT control; sedentary experimental diabetes and sedentary control. The animals in the HIIT group performed an aerobic exercise protocol on a treadmill inclined at an angle of 15° to the horizontal, with interspersed intensity. Five weekly sessions, lasting 49 min each, were held for 6 weeks. The analysis of cortical bone density (CBD) and BMD were performed by X-ray images using the In-Vivo Xtreme II/Bruker system. Results: For CBD and BMD, when comparing diabetes and control groups, a significant difference was seen between groups in relation to HIIT (p = 0.007). Animals submitted and not submitted to HIIT in the same group showed a significant difference between groups in relation to diabetes (p < 0.001). Conclusions: The HIIT experimental diabetes group had increased CBD and BMD in comparison with the sedentary experimental diabetes group.

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Section: Methodsmentioning

confidence: 99%

Analysis of high-intensity interval training on bone mineral density in an experimental model of type 2 diabetes

et al. 2022

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The efficacy of using metformin and/or quercetin for amelioration of gamma-irradiation induced tongue toxicity in diabetic rats

Ahmed,

Bakr,

Rasmy

2024

BMC Oral Health

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Background Diabetes is a common disease that cancer patients may suffer from and may aggravate side effects of radiotherapy. This study aimed to detect whether metformin and/or quercetin will improve gamma-irradiation induced tongue toxicity in diabetic rats. Methods 35 male albino rats were divided into five groups; NOR no streptozotocin, no radiation and no treatment was given, DR rats were subjected to streptozotocin then gamma-irradiation, DRM rats were subjected to streptozotocin then gamma-irradiation then metformin, DRQ rats were subjected to streptozotocin then gamma-irradiation then quercetin, DRMQ rats were subjected to streptozotocin then gamma-irradiation then metformin and quercetin. Rats were euthanized 24 h after last treatment dose. Mean blood glucose level was recorded. Tongue specimens were stained with H&E and CD68. Histomorphometric analysis of length, diameter and taste buds of lingual papillae and epithelial, keratin and lamina propria thickness and CD68 positive cells were calculated. Results Blood glucose level of DRMQ was significantly lower than DR, DRM and DRQ, whereas higher than NOR. Metformin or quercetin partially restored tongue structure, papillae length and diameter and tongue layers thickness. The ameliorative effect was superior when metformin and quercetin were used together. Diabetes and irradiation significantly increased number of CD68 positive macrophages in submucosa and muscles. Metformin or quercetin significantly reduced number of lingual macrophages with more noticeable effect for quercetin. Treatment with metformin and quercetin significantly decreased number of macrophages. Conclusions Combined use of metformin and quercetin might help mitigate the harmful effects of radiotherapy and diabetes on lingual tissues.

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Novel Approaches in Chronic Renal Failure without Renal Replacement Therapy: A Review

Martínez-Hernández,

Muñoz-Ortega,

Ávila-Blanco

et al. 2023

Biomedicines

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Chronic kidney disease (CKD) is characterized by renal parenchymal damage leading to a reduction in the glomerular filtration rate. The inflammatory response plays a pivotal role in the tissue damage contributing to renal failure. Current therapeutic options encompass dietary control, mineral salt regulation, and management of blood pressure, blood glucose, and fatty acid levels. However, they do not effectively halt the progression of renal damage. This review critically examines novel therapeutic avenues aimed at ameliorating inflammation, mitigating extracellular matrix accumulation, and fostering renal tissue regeneration in the context of CKD. Understanding the mechanisms sustaining a proinflammatory and profibrotic state may offer the potential for targeted pharmacological interventions. This, in turn, could pave the way for combination therapies capable of reversing renal damage in CKD. The non-replacement phase of CKD currently faces a dearth of efficacious therapeutic options. Future directions encompass exploring vaptans as diuretics to inhibit water absorption, investigating antifibrotic agents, antioxidants, and exploring regenerative treatment modalities, such as stem cell therapy and novel probiotics. Moreover, this review identifies pharmaceutical agents capable of mitigating renal parenchymal damage attributed to CKD, targeting molecular-level signaling pathways (TGF-β, Smad, and Nrf2) that predominate in the inflammatory processes of renal fibrogenic cells.

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Combination of cafeteria diet with intraperitoneally streptozotocin in rats. A type-2 diabetes model

Cited by 3 publications

References 45 publications

Analysis of high-intensity interval training on bone mineral density in an experimental model of type 2 diabetes

Analysis of high-intensity interval training on bone mineral density in an experimental model of type 2 diabetes

The efficacy of using metformin and/or quercetin for amelioration of gamma-irradiation induced tongue toxicity in diabetic rats

Novel Approaches in Chronic Renal Failure without Renal Replacement Therapy: A Review

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