Combination Effect of Hyperthermia and Photodynamic Therapy on Carcinoma

Abstract

We investigated the time course of hematoporphyrin oligomer uptake in squamous cell carcinoma transplanted into C3H mice after intraperitoneal injection by measuring its fluorescence intensity. The intensity peaked 83 hours after the injection. The tumors disappeared after an average of 4.12 days with a single application of photodynamic therapy or microwave hyperthermia 83 hours after hematoporphyrin oligomer injection. However, all the tumors treated with these single modalities regrew within 4 weeks after the treatments. Therefore, we investigated the therapeutic effect of microwave hyperthermia in combination with photodynamic therapy. As a result, the combination effect was strongest when high-dose therapy was used as the first choice, irrespective of the order of the treatments, and no tumor regrowth occurred for 45 days.

Combination Effect of Photodynamic and Sonodynamic Therapy on Experimental Skin Squamous Cell Carcinoma in C3H/HeN Mice

“…A number of studies have indicated enhanced tumor cytotoxicity when PDT is combined with hyperthermia, chemotherapy, or radiation (14)(15)(16)(17)(18). However, no studies have been conducted using the combination of PDT and SDT in animal experiments.…”

Apoptosis-promoting gene (bax) transfer potentiates sensitivity of squamous cell carcinoma to cisplatinIn vitro andIn vivo

“…Seven to 9 week‐old C3H/He (C3H) mice bearing SCC were used in all experiments (Matsumoto et al , 1990). A tumor from the donor mouse was cut into small pieces (about 0.2 g) and one of these pieces was transplanted s.c. into the back of a recipient mouse.…”

PRECLINICAL EXAMINATION OF FIRST and SECOND GENERATION PHOTOSENSITIZERS USED IN PHOTODYNAMIC THERAPY

“…Interestingly, light used to active the photosensitizer (630 nm) and light used to produce in vivo hyperthermia (1060 nm) can be delivered through the same quartz fiber. The combination of PDT and microwave generated hyperthermia has also been shown to be effective in a mouse tumor model (Matsumoto et al, 1990). In vivo interactions of combined modality therapy using interstitial applications of ionizing radiation, hyperthermia and PDT has been examined by Levendag et al (1989).…”