Combating antibiotic resistance using wastewater surveillance: Significance, applications, challenges, and future directions

Gholipour, Sahar; Shamsizadeh, Zahra; Halabowski, Dariusz; Gwenzi, Willis; Nikaeen, Mahnaz

doi:10.1016/j.scitotenv.2023.168056

Cited by 3 publications

(3 citation statements)

References 80 publications

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“…Studies have shown that advanced treatment systems can effectively eliminate antibiotic-resistant microorganisms (Chen et al 2015a(Chen et al , 2015b. At the parallel, surveillance programs for greywater quality, in particular, for the detection of antibiotic-resistant bacteria, can enable rapid intervention in the event of a risk to public health (Gholipour et al 2024). In addition, campaigns to raise awareness of good hygiene practices and the appropriate use of antibiotics can help to reduce the selection pressure for antibiotic resistance (Versporten et al 2018).…”

Section: Phenotypic Antimicrobial Susceptibility Of Presumptive Staph...mentioning

confidence: 99%

Occurrence of antibiotic-resistant bacteria in household greywater discharged into the environment in Burkina Faso's Sahel region

Compaoré,

Maiga,

Zongo

et al. 2024

Journal of Water and Health

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The prevalence and antibiotic resistance of Salmonella spp. and Staphylococcus spp. from greywater were investigated in Africa's Sahel region, Burkina Faso. A total of 36 and 56 isolates of Salmonella spp. and Staphylococcus spp. were isolated from greywater, respectively. From the Salmonella spp. isolates, resistance was most frequently observed against vancomycin (69%), ampicillin (61%), cefoxitin (42%), trimethoprine/sulfamethoxazole (36%), amoxicillin-clavunal acid (33%) and tetracycline (33%). For all Staphylococcus spp. isolates, the highest rate of resistance was against penicillin (55.36%) followed by oxacillin (48.21%) and clindamycin (30.36%). In addition, 75% of the isolated Salmonella spp. strains were resistant to at least two antibiotics of different families, while 30.35% of Staphylococcus spp. strains were multidrug-resistant (MDR). Overall, this is one of the first studies reporting the presence of MDR bacteria in untreated greywater discharged from domestic activities in Burkina Faso. Our results show that untreated greywater can contain bacteria resistant to antibiotics used in therapeutic care. Therefore, uncontrolled discharge of untreated greywater into the environment could lead to the dissemination of resistant bacteria and resistance genes in the environment and increase the risk of human exposure to antimicrobial resistance.

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Section: Phenotypic Antimicrobial Susceptibility Of Presumptive Staph...mentioning

confidence: 99%

Occurrence of antibiotic-resistant bacteria in household greywater discharged into the environment in Burkina Faso's Sahel region

Compaoré,

Maiga,

Zongo

et al. 2024

Journal of Water and Health

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“…In response to this challenge, current efforts have turned towards Wastewater-based Epidemiology (WBE) ([ 5 , 6 ]; [ 7 ]; [ 8 ]). WBE is a novel approach that analyses pharmaceutical residues in urban wastewater to gather real-time, non-invasive insights into community-wide drug consumption patterns [ 9 , 10 ,60]. This method has the potential to offer a more precise and inclusive understanding of antibiotic use, which is particularly beneficial in areas where healthcare access is sporadic or non-existent [ [11] , [12] , [13] ].…”

Section: Introductionmentioning

confidence: 99%

Optimisation of analytical methods for tuberculosis drug detection in wastewater: A multinational study

Mtetwa,

Amoah,

Kumari

et al. 2024

Heliyon

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“…As confirmed in an exemplary manner during the SARS-CoV-2 pandemic, wastewater monitoring is a helpful tool to evaluate the epidemiological situation in the catchment of treatment plants and to characterize the evolution processes of microorganisms [ 1 ]. This includes the presence of resistance determinants among fecal bacteria posing an increasing risk for patients, as well as the wastewater facilitating the dissemination of resistant species and their antibiotic-resistance genes in the aqueous environment [ 2 , 3 ].…”

Section: Introductionmentioning

confidence: 99%

Enterococci, Van Gene-Carrying Enterococci, and Vancomycin Concentrations in the Influent of a Wastewater Treatment Plant in Southeast Germany

Geissler,

Schröttner,

Oertel

et al. 2024

Microorganisms

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Vancomycin-resistant (VR) Enterococcus spp. can be detected in high concentrations in wastewaters and pose a risk to public health. During a one-year study (September 2022–August 2023), 24 h composite raw wastewater samples (n = 192) of a municipal wastewater treatment plant were investigated for cultivable enterococci. After growth on Slanetz–Bartley agar (SBA), a mean concentration of 29,736 ± 9919 cfu/mL was calculated. Using MALDI-TOF MS to characterize randomly picked colonies (n = 576), the most common species were found to be Enterococcus faecium (72.6%), E. hirae (13.7%), and E. faecalis (8.0%). Parallel incubation of wastewater samples on SBA and VRESelect agar resulted in a mean rate of VR enterococci of 2.0 ± 1.5%. All the tested strains grown on the VRESelect agar (n = 172) were E. faecium and carried the vanA (54.6%) or vanB gene (45.4%) with limited sequence differences. In susceptibility experiments, these isolates showed a high-level resistance to vancomycin (>256 µg/mL). Concentration of vancomycin was determined in 93.7% of 112 wastewater samples (mean: 123.1 ± 64.0 ng/L) and varied between below 100 ng/L (the detection limit) and 246.6 ng/L. A correlation between the concentration of vancomycin and the rate of VR strains among the total enterococci could not be found. The combination of incubation of samples on SBA and a commercial vancomycin-containing agar applied in clinical microbiology with a multiplex PCR for detection of van genes is an easy-to-use tool to quantify and characterize VR Enterococcus spp. in water samples.

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Combating antibiotic resistance using wastewater surveillance: Significance, applications, challenges, and future directions

Cited by 3 publications

References 80 publications

Occurrence of antibiotic-resistant bacteria in household greywater discharged into the environment in Burkina Faso's Sahel region

Occurrence of antibiotic-resistant bacteria in household greywater discharged into the environment in Burkina Faso's Sahel region

Optimisation of analytical methods for tuberculosis drug detection in wastewater: A multinational study

Enterococci, Van Gene-Carrying Enterococci, and Vancomycin Concentrations in the Influent of a Wastewater Treatment Plant in Southeast Germany

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