Colorimetric Determination of Reducing Sugars with Triphenyltetrazolium Chloride.

Mattson, A. M.; Jensen, C. O.

doi:10.1021/ac60037a038

Cited by 61 publications

(13 citation statements)

References 1 publication

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“…Neither halide nor sulfur was detected following sodium fusion, but calcium and magnesium were found to be present using the method of Sommer (1955). The lipid was tested for the presence of a reducing end group in the following manner, which is a modification of the method of Mattson and Jensen (1950). A-III was dissolved in pyridine and applied to Whatman No.…”

Section: Experimental Procedures and Resultsmentioning

confidence: 99%

Purification and Characterization of the Lipid A Component of the Lipopolysaccharides from Escherichia coli^*

Burton¹,

Carter²

1964

Biochemistry

143

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A crude lipopolysaccharide fraction has been isolated from Escherichia coli, strain OmB4, by cold aqueous phenol extraction. Purification of this material gave a lipopolysaccharide preparation (F-III) of high molecular weight in 4-5% yield (based on the dry weight of the cells).In addition, by extending the purification procedures commonly used, another 10-15 % yield of lipopolysaccharide was recovered. The lipid content of this second preparation (F-Vl) has not been previously observed. Mild aqueous acid degradation of the bacterial lipopolysaccharides

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Section: Experimental Procedures and Resultsmentioning

confidence: 99%

Purification and Characterization of the Lipid A Component of the Lipopolysaccharides from Escherichia coli^*

Burton¹,

Carter²

1964

Biochemistry

143

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“…Colorimetric chemical tests (see the Supporting Information for details) provided additional confirmation of chemical modification. A test with 2,3,5-triphenyltetrazolium chloride (TTC) , estimated the number of aldehyde groups for (P-Ox) to be 23 ± 3 nmol mg –1 cellulose substrate. After reduction of (P-SS), the number of thiol groups in (P-SH) was estimated to be 16 ± 3 nmol mg –1 substrate using a test with Ellman’s reagent …”

Section: Resultsmentioning

confidence: 99%

Affinity Immobilization of Semiconductor Quantum Dots and Metal Nanoparticles on Cellulose Paper Substrates

Kim

Tran

Petryayeva

et al. 2020

ACS Appl. Mater. Interfaces

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Colloidal semiconductor quantum dots (QDs), metal nanoparticles, and cellulose paper are materials with numerous applications in bioanalysis and beyond. The functional properties of QDs and metal NPs are substantially different than those of cellulose, such that their integration with cellulose paper is potentially enabling for many applications. Here, we characterize and evaluate multiple chemistries that modify cellulose paper substrates for the affinity-based immobilization of QDs, gold nanoparticles (Au NPs), and platinum nanoparticles (Pt NPs). These chemistries include grafting of cellulose fibers with imidazole and dithiol groups, as well as the aminosilanization of cellulose fibers (both with and without subsequent grafting with dithiol groups). Cellulose modifications and nanoparticle immobilization are characterized by multiple techniques, including, but not limited to, X-ray photoelectron spectroscopy, scanning electron microscopy, and optical imaging, extinction, and fluorescence measurements. We demonstrate the on-paper immobilization of color-tuned mixtures of QDs, on-paper patterning of QDs by microcontact printing, and post-immobilization enhancement of energy transfer and model assays of protease activity. The robustness of QD photoluminescence is also evaluated between immobilization chemistries. Paper-immobilized Au NPs and Pt NPs are evaluated as potential substrates for SERS and as supported catalysts for a model decolorization reaction. Our cumulative results indicate that there may not be a one-size-fits-all immobilization chemistry. Instead, the immobilization chemistry should be tailored and optimized for the downstream application.

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“…The measurements are achieved taking advantage of the fact that carbonyls are able, in alkaline environment, to reduce 2,3,5-triphenyl-2H-tetrazolium chloride to the corresponding triphenylformazan showing a red colour. [10] The amount of formazan formed is directly proportional to the amount of carbonyl groups present, and it is spectrophotometrically determined at 480 nm.…”

Section: Methodsmentioning

confidence: 99%

Atomic force microscopy application for degradation diagnostics in library heritage

Coluzza

Bicchieri²,

Monti³

et al. 2008

Surface & Interface Analysis

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Atomic Force Microscopy was used on pure cotton cellulose paper to characterize the relationship between surface topographies and cellulose degradation patterns. Whatman N.1 chromatography paper and Fabriano Umbria paper artificially aged with different procedures and then naturally aged-were evaluated together with a sample from an original book. Evidence is found of a clear distinction between surface heights distribution of chiefly oxidised samples with respect to those where hydrolysis is the dominant process. In both cases, deteriorated paper shows surface features well different from the paper in good preservation state, in agreement to results from previous experiments. This is a first step that could open the way to AFM application as a qualitative diagnostic tool for library materials. The only way to achieve such a goal is to extend the experimentation to a wider population of paper samples, so to improve the statistical evaluation.

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Colorimetric Determination of Reducing Sugars with Triphenyltetrazolium Chloride.

Cited by 61 publications

References 1 publication

Purification and Characterization of the Lipid A Component of the Lipopolysaccharides from Escherichia coli^*

Purification and Characterization of the Lipid A Component of the Lipopolysaccharides from Escherichia coli^*

Affinity Immobilization of Semiconductor Quantum Dots and Metal Nanoparticles on Cellulose Paper Substrates

Atomic force microscopy application for degradation diagnostics in library heritage

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Colorimetric Determination of Reducing Sugars with Triphenyltetrazolium Chloride.

Cited by 61 publications

References 1 publication

Purification and Characterization of the Lipid A Component of the Lipopolysaccharides from Escherichia coli*

Purification and Characterization of the Lipid A Component of the Lipopolysaccharides from Escherichia coli*

Affinity Immobilization of Semiconductor Quantum Dots and Metal Nanoparticles on Cellulose Paper Substrates

Atomic force microscopy application for degradation diagnostics in library heritage

Contact Info

Product

Resources

About

Purification and Characterization of the Lipid A Component of the Lipopolysaccharides from Escherichia coli^*

Purification and Characterization of the Lipid A Component of the Lipopolysaccharides from Escherichia coli^*