Colorimetric detection of active botulinum neurotoxin using Cu2+ mediated gold nanoparticles agglomeration

Chen, Shan; Chu, Lok Ting; Chen, Ting‐Hsuan

doi:10.1016/j.snb.2016.05.118

Cited by 10 publications

(10 citation statements)

References 27 publications

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Section: Methods Of Detectionmentioning

confidence: 99%

“…A similar methodology was developed utilising the same basic platform: BoNT cleaved peptides leading to AuNP agglomeration, facilitating a visually observable colour shift from red to blue [117]. The assay allowed for clear visually distinguishable responses at concentrations reaching as little as 1 ng/mL with a LoD around 250 pg/mL [117]. This method utilises each peptide fragment in a ‘turn-on’ method, where instead of the BoNT breaking a peptide linkage, destabilising the AuNPs (a ‘turn-off’ process), it uses Cu 2+ to form a chemical complex with the post cleavage peptide fragments.…”

Section: Methods Of Detectionmentioning

confidence: 99%

“…This method utilises each peptide fragment in a ‘turn-on’ method, where instead of the BoNT breaking a peptide linkage, destabilising the AuNPs (a ‘turn-off’ process), it uses Cu 2+ to form a chemical complex with the post cleavage peptide fragments. This method is more efficient at inducing particle agglomeration which leads to the detection of lower concentrations of BoNT [117]. The main advantage of this methodology is simplicity of the assays coupled with the relatively high sensitivity; however, there are increased costs compared to the previously described colorimetric assay due to the use of AuNPs, Cu 2+ and modified magnetic micro-particles [117].…”

Section: Methods Of Detectionmentioning

confidence: 99%

“…This method is more efficient at inducing particle agglomeration which leads to the detection of lower concentrations of BoNT [117]. The main advantage of this methodology is simplicity of the assays coupled with the relatively high sensitivity; however, there are increased costs compared to the previously described colorimetric assay due to the use of AuNPs, Cu 2+ and modified magnetic micro-particles [117]. It also, like most assays, has not been fully tested against various BoNT serotypes and in a range of sample matrices.…”

Section: Methods Of Detectionmentioning

confidence: 99%

See 3 more Smart Citations

Rapid Detection of Botulinum Neurotoxins—A Review

Hobbs

Thomas

Halliwell

et al. 2019

Toxins

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A toxin is a poisonous substance produced within living cells or organisms. One of the most potent groups of toxins currently known are the Botulinum Neurotoxins (BoNTs). These are so deadly that as little as 62 ng could kill an average human; to put this into context that is approximately 200,000 × less than the weight of a grain of sand. The extreme toxicity of BoNTs leads to the need for methods of determining their concentration at very low levels of sensitivity. Currently the mouse bioassay is the most widely used detection method monitoring the activity of the toxin; however, this assay is not only lengthy, it also has both cost and ethical issues due to the use of live animals. This review focuses on detection methods both existing and emerging that remove the need for the use of animals and will look at three areas; speed of detection, sensitivity of detection and finally cost. The assays will have wide reaching interest, ranging from the pharmaceutical/clinical industry for production quality management or as a point of care sensor in suspected cases of botulism, the food industry as a quality control measure, to the military, detecting BoNT that has been potentially used as a bio warfare agent.

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Section: Methods Of Detectionmentioning

confidence: 99%

Section: Methods Of Detectionmentioning

confidence: 99%

Section: Methods Of Detectionmentioning

confidence: 99%

Section: Methods Of Detectionmentioning

confidence: 99%

See 2 more Smart Citations

Rapid Detection of Botulinum Neurotoxins—A Review

Hobbs

Thomas

Halliwell

et al. 2019

Toxins

View full text Add to dashboard Cite

show abstract

“…al. [95] developed a new and easy to use colorimetric detection of functional BoNT mediated by gold nanoparticles agglomeration. This work is based on the ability of active BoNT to enzymatically cleave SNAptide®, a synthetic peptide that acts as substrate.…”

mentioning

confidence: 99%