Color Reaction of Hexuronic Acids with Anthrone

Helbert, J.; Brown, Keith

doi:10.1021/ac60115a009

Cited by 32 publications

(5 citation statements)

References 5 publications

(5 reference statements)

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“…Solomons and Irving relied on the lysine and hydroxylysine content values reported by Hess and Lee (1954) and Bowes et al (1957) for their comparison. FDNB reactivity data are itemized in Table IV.…”

Section: Resultsmentioning

confidence: 99%

The Macromolecular Organization of Dentine Matrix Collagen. I. Characterization of Dentine Collagen^*

Veis

Schlueter²

1964

Biochemistry

137

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Section: Resultsmentioning

confidence: 99%

The Macromolecular Organization of Dentine Matrix Collagen. I. Characterization of Dentine Collagen^*

Veis

Schlueter²

1964

Biochemistry

137

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“…The samples were allowed to react for 10 min, and the absorbance at 630 nm was read (Spectronic GENESYS 20) and compared against glucose standards. Final sugar concentrations were reported in equivalent glucose units (34, 35). Polysaccharide determination was performed in triplicate (Table 1).…”

Section: Methodsmentioning

confidence: 99%

A Distinguishable Role of eDNA in the Viscoelastic Relaxation of Biofilms

Peterson

Mei

Sjollema

et al. 2013

mBio

100

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Bacteria in the biofilm mode of growth are protected against chemical and mechanical stresses. Biofilms are composed, for the most part, of extracellular polymeric substances (EPSs). The extracellular matrix is composed of different chemical constituents, such as proteins, polysaccharides, and extracellular DNA (eDNA). Here we aimed to identify the roles of different matrix constituents in the viscoelastic response of biofilms. Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus mutans, and Pseudomonas aeruginosa biofilms were grown under different conditions yielding distinct matrix chemistries. Next, biofilms were subjected to mechanical deformation and stress relaxation was monitored over time. A Maxwell model possessing an average of four elements for an individual biofilm was used to fit the data. Maxwell elements were defined by a relaxation time constant and their relative importance. Relaxation time constants varied widely over the 104 biofilms included and were divided into seven ranges (<1, 1 to 5, 5 to 10, 10 to 50, 50 to 100, 100 to 500, and >500 s). Principal-component analysis was carried out to eliminate related time constant ranges, yielding three principal components that could be related to the known matrix chemistries. The fastest relaxation component (<3 s) was due to the presence of water and soluble polysaccharides, combined with the absence of bacteria, i.e., the heaviest masses in a biofilm. An intermediate component (3 to 70 s) was related to other EPSs, while a distinguishable role was assigned to intact eDNA, which possesses a unique principal component with a time constant range (10 to 25 s) between those of EPS constituents. This implies that eDNA modulates its interaction with other matrix constituents to control its contribution to viscoelastic relaxation under mechanical stress.

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“…3 Durch die Varianzanalyse der Abweichungen der festgestellten Werte von den wirklichen Werten zeigt sich, daS diese nicht von der Zusammensetzung der Mischung abhangen. Es werden einige systematische Abweichungen festgestellt, die auf die anschliel3end erorterte Art korrigiert werden.…”

Section: Experimentelder Teilunclassified

Spektrophotometrische Bestimmung des Pektins und begleitender Polysaccaride

Zitko

Rosík

1961

Nahrung

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Color Reaction of Hexuronic Acids with Anthrone

Cited by 32 publications

References 5 publications

The Macromolecular Organization of Dentine Matrix Collagen. I. Characterization of Dentine Collagen^*

The Macromolecular Organization of Dentine Matrix Collagen. I. Characterization of Dentine Collagen^*

A Distinguishable Role of eDNA in the Viscoelastic Relaxation of Biofilms

Spektrophotometrische Bestimmung des Pektins und begleitender Polysaccaride

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Color Reaction of Hexuronic Acids with Anthrone

Cited by 32 publications

References 5 publications

The Macromolecular Organization of Dentine Matrix Collagen. I. Characterization of Dentine Collagen*

The Macromolecular Organization of Dentine Matrix Collagen. I. Characterization of Dentine Collagen*

A Distinguishable Role of eDNA in the Viscoelastic Relaxation of Biofilms

Spektrophotometrische Bestimmung des Pektins und begleitender Polysaccaride

Contact Info

Product

Resources

About

The Macromolecular Organization of Dentine Matrix Collagen. I. Characterization of Dentine Collagen^*

The Macromolecular Organization of Dentine Matrix Collagen. I. Characterization of Dentine Collagen^*