Colocalization of growth hormone (GH) and glycoprotein subunit ? in GH-producing pituitary adenomas in acromegalic patients

Furuhata, Shigeru; Kameya, Toru; Tsuruta, Tomoko; Naritaka, Heiji; Toya, Shigeo

doi:10.1007/bf00293316

Cited by 12 publications

(3 citation statements)

References 12 publications

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“…Monomorphous adenomas are defined as those that synthesize two or more hormones in the same cell. In bimorphous and plurimorphous adenomas, each hormone is produced by different cell populations, and almost all possible combinations of hormone productions involving GH and PRL, GH, PRL, glycoprotein hormone subunit ( GSU), TSH or FSH have been observed (Felix et al 1994, Furuhata et al 1994, Gessl et al 1994, Gil-del-Alamo et al 1994, Bertholon-Gregoire et al 1999. Although these combinations of hormones correspond to the differentiation of the cell lineages, it has been reported that very occasional cases of bimorphic pituitary adenomas produce adrenocorticotropic hormone (ACTH) and GH in different cell populations (Arita et al 1991, Kovacs et al 1998.…”

Section: Introductionmentioning

confidence: 99%

Exogenous expression of Pit-1 in AtT-20 corticotropic cells induces endogenous growth hormone gene transcription

Kurotani

Yoshimura²,

Iwasaki³

et al. 2002

Journal of Endocrinology

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The pituitary-specific POU-homeodomain transcription factor, Pit-1, is known to regulate the expression of the GH gene in somatotropes, prolactin (PRL) in lactotropes, and TSH in thyrotropes. It is not normally expressed in corticotropes or gonadotropes. We addressed the question of whether exogenous Pit-1 was sufficient to induce ectopic transcription of the GH gene in the corticotropic cell line, AtT-20, or the gonadotropic cell line, T3-1. A fusion gene composed of enhanced green fluorescent protein gene and human Pit-1 cDNA was transfected into AtT-20 and T3-1 cells. The endogenous mouse GH mRNA was induced in three of nine AtT-20 cell lines and one of three T3-1 cell lines containing the fusion gene. A small amount of GH protein was also detected in these cell lines. These data indicate that transfected Pit-1 is capable of inducing transcription of the GH gene in AtT-20 cells and T3-1 cells. These data also suggest that synergistic co-factors might be required to transcribe the GH gene effectively for translation into GH protein. Furthermore, our findings support the hypothesis that the function of anterior pituitary cells is determined by the combinatorial action of specific transcription factors.

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Section: Introductionmentioning

confidence: 99%

Exogenous expression of Pit-1 in AtT-20 corticotropic cells induces endogenous growth hormone gene transcription

Kurotani

Yoshimura²,

Iwasaki³

et al. 2002

Journal of Endocrinology

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“…In a further tumour, the acidophil stem cell adenoma, GH is co-expressed with PRL although it is rarely secreted in excess . Growth hormone-secreting tumours are also associated with the expression of thyroidstimulating hormone (TSH) or the common ␣-subunit of the glycoprotein hormones (Furuhata et al, 1994;Kovacs et al, 1982;Osamura and Watanabe, 1987;White et al, 1986). These associations reflect the Pit-1 regulated cell lineage in the normal gland.…”

Section: Pituitary Adenomas Classical Pituitary Hormonesmentioning

confidence: 96%

Gene expression in pituitary adenomas: New insights

McNicol

1997

Microsc. Res. Tech.

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“…Multihormone production in monomorphous adenomas frequently includes combinations of GH, PRL, and TSHβ, or FSHβ and LHβ, which are derived from the same cell lineage that is dependent on Pit-1 and SF-1/GATA-2, respectively. In bimorphous and plurimorphous adenomas, each hormone is produced by a different cell population, and almost all possible combinations of hormone production involving GH and PRL, GH, PRL, glycoprotein hormone α subunit (αSU), TSHβ, and FSHβ have been observed (Felix et al 1994;Furuhata et al 1994;Gessl et al 1994;Gil del Alamo et al 1994;Bertholon Gregoire et al 1999).…”

Section: Introductionmentioning

confidence: 96%

Induction of GH, PRL, and TSHβ mRNA by transfection of Pit-1 in a human pituitary adenoma-derived cell line

et al. 2005

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The functional development of pituitary cells depends on the expression of a combination of transcription factors and co-factors. Pituitary-specific transcription factor-1 (Pit-1) is required for the expression of growth hormone (GH), prolactin (PRL), and the thyroid-stimulating hormone beta subunit (TSH beta) and acts synergistically with the estrogen receptor (ER) and GATA-binding protein 2 (GATA-2) to induce PRL and TSH beta expression, respectively. The glycoprotein hormone alpha subunit (alpha SU) is the first hormone to be expressed during pituitary development. In addition to being expressed in follicle-stimulating hormone, luteinizing hormone (LH), and TSH cells, alpha SU is reported to co-localize with GH in pituitary cells. These findings have led to the suggestion that the expression of Pit-1 in cells of the alpha SU-based gonadotropin cell lineage might also lead to the expression of GH. In this study, we transfected HP 75 cells (derived from a human non-functioning pituitary adenoma that expressed alpha SU and LH beta) with Pit-1 by using an adenovirus FLAG-Pit-1 construct. Most of the transfected cells expressed GH mRNA, with fewer cells expressing PRL and TSH beta mRNA. The HP 75 cells expressed the genes for ER and GATA-2, thus allowing their expression of GH, PRL, and TSH beta mRNA in response to Pit-1. These results support the hypothesis that GH can be induced in cells that possess an active alpha SU gene and shed light on the basic molecular mechanism that drives the development of GH, PRL, and TSH beta expression in the alpha SU-based gonadotroph lineage.

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Colocalization of growth hormone (GH) and glycoprotein subunit ? in GH-producing pituitary adenomas in acromegalic patients

Cited by 12 publications

References 12 publications

Exogenous expression of Pit-1 in AtT-20 corticotropic cells induces endogenous growth hormone gene transcription

Exogenous expression of Pit-1 in AtT-20 corticotropic cells induces endogenous growth hormone gene transcription

Gene expression in pituitary adenomas: New insights

Induction of GH, PRL, and TSHβ mRNA by transfection of Pit-1 in a human pituitary adenoma-derived cell line

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