Collagen morphology and texture analysis: from statistics to classification

Mostaço-Guidolin, Leila B.; Ko, Alex C.‐T.; Wang, Fei; Xiang, Bo; Hewko, Mark; Tian, Ganghong; Major, Arkady; Shiomi, Masashi; Sowa, Michael G.

doi:10.1038/srep02190

Cited by 134 publications

(150 citation statements)

References 65 publications

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“…Forty-two images from each gel were quantified, with six gels per condition. Skewness decreases as fibril organization increases (48). Box and whisker plots as in Fig.…”

Section: Fibroblasts Are Activated To Myofibroblasts By Tumor-associamentioning

confidence: 93%

“…Skewness is a measure of the imbalance of the distribution of a variable about its mean, in this case pixels that are darker or brighter than the image mean. Images with greater organization, such as those with large fibrils, exhibit a left-tailed shift in the distribution that is calculated as a negative shift in skewness (48). Both hydrogel types show a significant negative shift in skewness from day 0 to day 3, suggesting that the matrices are being organized by the embedded cells (Fig.…”

Section: Fibroblasts Are Activated To Myofibroblasts By Tumor-associamentioning

confidence: 96%

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Palladin Mediates Stiffness-Induced Fibroblast Activation in the Tumor Microenvironment

McLane

Ligon

2015

Biophysical Journal

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Mechanical properties of the tumor microenvironment have emerged as key factors in tumor progression. It has been proposed that increased tissue stiffness can transform stromal fibroblasts into carcinoma-associated fibroblasts. However, it is unclear whether the three to five times increase in stiffness seen in tumor-adjacent stroma is sufficient for fibroblast activation. In this study we developed a three-dimensional (3D) hydrogel model with precisely tunable stiffness and show that a physiologically relevant increase in stiffness is sufficient to lead to fibroblast activation. We found that soluble factors including CC-motif chemokine ligand (CCL) chemokines and fibronectin are necessary for this activation, and the combination of C-C chemokine receptor type 4 (CCR4) chemokine receptors and β1 and β3 integrins are necessary to transduce these chemomechanical signals. We then show that these chemomechanical signals lead to the gene expression changes associated with fibroblast activation via a network of intracellular signaling pathways that include focal adhesion kinase (FAK) and phosphoinositide 3-kinase (PI3K). Finally, we identify the actin-associated protein palladin as a key node in these signaling pathways that result in fibroblast activation.

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“…Forty-two images from each gel were quantified, with six gels per condition. Skewness decreases as fibril organization increases (48). Box and whisker plots as in Fig.…”

Section: Fibroblasts Are Activated To Myofibroblasts By Tumor-associamentioning

confidence: 93%

Section: Fibroblasts Are Activated To Myofibroblasts By Tumor-associamentioning

confidence: 96%

Palladin Mediates Stiffness-Induced Fibroblast Activation in the Tumor Microenvironment

McLane

Ligon

2015

Biophysical Journal

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“…Then, the resulting magnitude of the SHG signal has quadratic dependence on the number of molecules and the intensity of the incident light. 18 The molecular nature of type I collagen enables SHG emission, and SHG microscopy has therefore become an elementary tool for type I collagen evaluation.…”

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confidence: 99%

Osteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films

Lišková¹,

Babchenko²,

Varga³

et al. 2015

IJN

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Nanocrystalline diamond (NCD) films are promising materials for bone implant coatings because of their biocompatibility, chemical resistance, and mechanical hardness. Moreover, NCD wettability can be tailored by grafting specific atoms. The NCD films used in this study were grown on silicon substrates by microwave plasma-enhanced chemical vapor deposition and grafted by hydrogen atoms (H-termination) or oxygen atoms (O-termination). Human osteoblast-like Saos-2 cells were used for biological studies on H-terminated and O-terminated NCD films. The adhesion, growth, and subsequent differentiation of the osteoblasts on NCD films were examined, and the extracellular matrix production and composition were quantified. The osteoblasts that had been cultivated on the O-terminated NCD films exhibited a higher growth rate than those grown on the H-terminated NCD films. The mature collagen fibers were detected in Saos-2 cells on both the H-terminated and O-terminated NCD films; however, the quantity of total collagen in the extracellular matrix was higher on the O-terminated NCD films, as were the amounts of calcium deposition and alkaline phosphatase activity. Nevertheless, the expression of genes for osteogenic markers – type I collagen, alkaline phosphatase, and osteocalcin – was either comparable on the H-terminated and O-terminated films or even lower on the O-terminated films. In conclusion, the higher wettability of the O-terminated NCD films is promising for adhesion and growth of osteoblasts. In addition, the O-terminated surface also seems to support the deposition of extracellular matrix proteins and extracellular matrix mineralization, and this is promising for better osteoconductivity of potential bone implant coatings.

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“…Therefore, for each Harlick's feature, four different values and a total of 48 GLCM texture features are obtained. The formulas and a brief description of the Harlick's features with respect to the texture characterisation are presented in Table 1 [26,[30][31][32][33].…”

Section: Dissociation Object Model On Vois and Feature Extractionmentioning

confidence: 99%

Computer-Assisted Diagnosis System for Breast Cancer in Computed Tomography Laser Mammography (CTLM)

et al. 2017

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Computed tomography laser mammography (Eid et al. Egyp J Radiol Nucl Med, 37(1): p. 633-643, 1) is a non-invasive imaging modality for breast cancer diagnosis, which is time-consuming and challenging for the radiologist to interpret the images. Some issues have increased the missed diagnosis of radiologists in visual manner assessment in CTLM images, such as technical reasons which are related to imaging quality and human error due to the structural complexity in appearance. The purpose of this study is to develop a computer-aided diagnosis framework to enhance the performance of radiologist in the interpretation of CTLM images. The proposed CAD system contains three main stages including segmentation of volume of interest (VOI), feature extraction and classification. A 3D Fuzzy segmentation technique has been implemented to extract the VOI. The shape and texture of angiogenesis in CTLM images are significant characteristics to differentiate malignancy or benign lesions. The 3D compactness features and 3D Grey Level Co-occurrence matrix (GLCM) have been extracted from VOIs. Multilayer perceptron neural network (MLPNN) pattern recognition has developed for classification of the normal and abnormal lesion in CTLM images. The performance of the proposed CAD system has been measured with different metrics including accuracy, sensitivity, and specificity and area under receiver operative characteristics (AROC), which are 95.2, 92.4, 98.1, and 0.98%, respectively.

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Collagen morphology and texture analysis: from statistics to classification

Cited by 134 publications

References 65 publications

Palladin Mediates Stiffness-Induced Fibroblast Activation in the Tumor Microenvironment

Palladin Mediates Stiffness-Induced Fibroblast Activation in the Tumor Microenvironment

Osteogenic cell differentiation on H-terminated and O-terminated nanocrystalline diamond films

Computer-Assisted Diagnosis System for Breast Cancer in Computed Tomography Laser Mammography (CTLM)

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