Collagen from jumbo squid fin: extracting conditions and influence of the protease system on collagen hydrolysate antioxidant activity

Abstract

Collagen from jumbo squid fin: extracting conditions and influence of the protease system on collagen hydrolysate antioxidant activity Colágeno de aletas de calamar gigante: condiciones de extracción y efecto del sistema proteolítico en la actividad antioxidante de sus hidrolizados The optimal alkaline and acid conditions for insoluble collagen extraction from jumbo squid fin (JSF) were established by factorial analysis. Dependent variable: protein concentration; independent variables: NaOH and HCl concentrations. The antioxidant properties of JSF collagen hydrolysates obtained by two protease systems were studied. Moreover, jumbo squid skin (JSS) collagen was obtained and hydrolysated under optimal conditions. The optimal extraction condition was 0.5 M NaOH followed by 0.2 M HCl. Collagen α-chains were detected in both JSF and JSS. Collagen β-component was detected only in JSS. JSS collagen showed higher levels of polar and hydrophobic amino acids. The JSF hydrolysates produced by subtilisin showed a lower degree of hydrolysis and higher antioxidant activity (2,2-diphenyl-1-picrylhydrazyl (DPPH), Trolox equivalent antioxidant capacity (TEAC), and oxygen radical absorbance capacity-fluorescein (ORAC FL )) than those produced by a mixture of trypsin, chymotrypsin, and peptidase. The JSS hydrolysates showed a higher antioxidant capacity. We have thus established a suitable process to improve the utility of JSF.Keywords: fin collagen; extraction conditions; protease system; hydrolysate; antioxidant activity La mejores condiciones para la extracción del colágeno insoluble de las aletas del calmar gigante (JSF) fueron establecidas aplicando un diseño factorial. Variable dependiente: concentración de proteína; variables independientes: concentraciones de NaOH y HCl. Se comparó la actividad antioxidante de hidrolizados del colágeno de JSF, obtenidos mediante dos sistemas proteolíticos. Además, usando las mejores condiciones se extrajo el colágeno de la piel del calamar (JSS) e hidrolizó. Las mejores condiciones de extracción fueron: NaOH 0.5 M y HCl 0.2 M. Las bandas α se observaron en ambos colágenos. El β-componente de colágeno solo se detectó en JSS. JSS mostró mayor contenido de aminoácidos polares e hidrofóbicos. Los hidrolizados de JSF producidos por subtisilina presentaron menor grado de hidrólisis, la más alta actividad antioxidante (DPPH, TEAC, ORAC FL ) que los obtenidos por una mezcla de tripsina, quimotripsina y peptidasas. Los hidrolizados del JSS mostrarón la mayor actividad antioxidante. Se estableció un proceso para un mejor aprovechamiento de JSF.

Bioactive peptides from collagen hydrolysates from squid (Dosidicus gigas) by‐products fractionated by ultrafiltration

“…The percentage of collagen obtained after extraction was 64.5% with a degree of hydrolysis of 25.2%, similar values to those reported elsewhere (Torres‐Arreola et al ., ; Suárez‐Jiménez et al ., ; Cuevas‐Acuña et al ., ). The hydrolysate yield (expressed as grams of dry hydrolysate per 100 g of collagen) was 11.2%; these hydrolysates presented antioxidant activity measured in terms of DPPH and ABTS radical scavenging activities (Table ).…”

“…, lane SH), with the most intense bands located in the range <6.5 kDa. Comparable electrophoretic patterns have been reported for hydrolysates obtained out of collagen and gelatin from skin, fins and tentacle of Dosidicus gigas (Aleman et al ., ; Suárez‐Jiménez et al ., ; Cuevas‐Acuña et al ., ). The obtained hydrolysate was also rich in glycine, arginine, leucine, glutamic acid, aspartic acid and proline, as well as hydroxyproline (Table ), which is exclusive to collagen.…”

“…Collagen hydrolysates offer tremendous potential as new sources of bioactive peptides that display several biological properties (Samaranayaka & Li‐Chan, ). Enzymatic hydrolysates derived from squid by‐product collagen possess antioxidant, antihypertensive and anticancer properties (Aleman et al ., ; Suárez‐Jiménez et al ., ; Cuevas‐Acuña et al ., ). Furthermore, collagen hydrolysates obtained from squid by‐product have also been reported to exhibit antimutagenic activity (Suárez‐Jiménez et al ., ).…”

Obtaining glycoconjugates of marine origin via Maillard reaction and their cytotoxic effect: an alternative for the use of animal byproducts

“…An analysis of functional groups using FTIR spectroscopy was performed to confirm the glycation of the molecules of interest, since the OPA technique may have some limitations in the determination of degree of glycation because OPA and β ‐mercaptoethanol do not react with secondary amines such as proline and hydroxyproline, which are parts of the collagenous proteins from Dosidicus gigas . Figure shows the typical FTIR spectra for CTP 0 h, CTP 6 h and the glycoconjugates CTP‐GLU and CTP‐DEX with the different protein‐to‐carbohydrate ratios.…”

Xanthommatin is Behind the Antioxidant Activity of the Skin of Dosidicus gigas

“…Squid skin (normally considered a discard) is particularly rich in biologically active compounds, such as gelatin, collagen, and their peptides, as well as pigments [4,5,6,7]. The pigments found in jumbo squid skin are a part of its defense mechanism, which has been perfected through years of evolution.…”