2002
DOI: 10.1074/jbc.m206354200
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Abstract: Matrix metalloproteinase-14 is required for degradation of fibrillar collagen by mesenchymal cells. Here we show that keratinocytes use an alternative plasminogen and matrix metalloproteinase-13-dependent pathway for dissolution of collagen fibrils. Primary keratinocytes displayed an absolute requirement for serum to dissolve collagen. Dissolution of collagen was abolished in plasminogen-depleted serum and could be restored by the exogenous addition of plasminogen. Both plasminogen activator inhibitor-1 and ti… Show more

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Cited by 72 publications
(58 citation statements)
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“…Zymographic Analysis of the Mouse DESC1 Serine Protease Domain-Gelatin and casein zymography was performed on 11% Tris/ glycine gels containing either 0.5% gelatin or 0.1% ␤-casein (Sigma) as described previously (45). The gels were developed overnight in 0.1 M glycine, pH 8.0, at 37°C, stained with 0.1% Coomassie Blue, and destained in a 30% methanol and 10% acetic acid solution to visualize the zones of substrate lysis.…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Zymographic Analysis of the Mouse DESC1 Serine Protease Domain-Gelatin and casein zymography was performed on 11% Tris/ glycine gels containing either 0.5% gelatin or 0.1% ␤-casein (Sigma) as described previously (45). The gels were developed overnight in 0.1 M glycine, pH 8.0, at 37°C, stained with 0.1% Coomassie Blue, and destained in a 30% methanol and 10% acetic acid solution to visualize the zones of substrate lysis.…”
Section: Methodsmentioning
confidence: 99%
“…Analysis of DESC1 mRNA Expression in the Mouse-Primary mouse keratinocytes and fibroblasts were isolated from the epidermis of 1-dayold mice exactly as described (45). Total RNA from keratinocytes cultured for 3 days, fibroblasts, and 1-day-old mouse skin were isolated using Trizol reagent (Invitrogen), followed by sodium acetate/ethanol precipitation.…”
Section: Methodsmentioning
confidence: 99%
“…After gelling, 5 3 10 4 synoviocytes suspended in 50 ml serum-free medium were seeded into the center of each well and allowed to attach for 8 h (9,33). After the removal of nonadherent cells by washing, 0.5 ml serumfree medium was added to each well with or without IL-1b (1 nM), plasminogen (20 mg/ml; Calbiochem), the synthetic MMP inhibitor BB-94 (5 mM final concentration in 0.1% DMSO; British Biotechnology, Oxford, U.K.), the cysteine proteinase inhibitor, E-64d (100 mM; Sigma-Aldrich, St. Louis, MO), or the serine proteinase inhibitor aprotinin (200 mg/ml; Roche, Mannheim, Germany) as indicated (9).…”
Section: Collagen Degradation Assaysmentioning
confidence: 99%
“…This activated uPA complex then converts serum plasminogen to plasmin on the cell surface (48). Plasmin then mediates the proteolytic activation of transforming growth factor ␤ and matrix metalloproteinases in migration (49) and possibly gp140 to p80. A similar mechanism has been proposed for plasmin conversion of precursor laminin 5 to the mature laminin 5 in wounds as a regulator of migration (44,50).…”
mentioning
confidence: 99%