Cold Plasma Techniques for Pharmaceutical and Biomedical Engineering

Sasai, Yasushi; Kondo, Shinichi; Yamauchi, Yukiko; Kuzuya, Masayuki

doi:10.5772/13366

Cited by 1 publication

(2 citation statements)

References 46 publications

(26 reference statements)

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“…The application of plasma techniques for surface modification and subsequent immobilization of biomaterials has become increasingly common [5]. Cold Atmospheric Plasma is non-thermal flow and warms the surface to less than 40°C at the point of application.…”

Section: Discussionmentioning

confidence: 99%

“…Most of the polymers, commonly used for biomedical applications, need modifications in surface properties by chemical or physical tools, e.g., plasma. Plasma is the state of ionized gas that, based on the thermodynamic equilibrium of energy of its particles, is defined as hot or cold [5]. The latter is applied for the treatment of heat-sensitive objects such as polymeric materials and biological samples without introducing damages.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bioactivity Determination of Recombinant lysostaphin Immobilized on Glass Surfaces Modified by Cold Atmospheric Plasma on Staphylococcus aureus

Ehsani

Fahmide

Norouzian

et al. 2019

JoMMID

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Introduction: Staphylococcus aureus is a source of nosocomial infections and one of the significant concerns in patients with indwelling devices. Lysostaphin is a bacterially produced endopeptidase with a unique activity on S. aureus. Plasma, the fourth state of the material, consists of charged ions, free electrons, and activated neutral species. Biomedical applications of cold plasma are rapidly growing due to its capacity to treat heat-sensitive objects such as polymeric materials and biological samples. It activates surfaces by etching them to stabilize proteins. The direct effect of cold atmospheric plasma on the eradication of microorganisms have been investigated. However, there is no report on immobilizing antibiotic agents. Methods: In this study, the lysostaphin protein was expressed and purified using Ni-NTA column, then the purified enzyme was immobilized on glass surfaces pretreated with cold atmospheric plasma for 150 s, 200 s, and 300 s. The antimicrobial activity of immobilized lysostaphin on S. aureus was approved by in vitro analysis. Results: The 300 s plasma treatment confirmed to be the best time arrangement for more lysostaphin immobilization, shown by Atomic Force Microscopy. Conclusion: Our results showed that passive adsorption to the treated surface does not affect the structure and subsequent antimicrobial function of the recombinant protein compared to the standard proteins.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%